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Research article
Tao Huang
College of Animal Science and Technology, Shihezi University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2943-5131
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Ovulation rate is an extremely important factor of litter size in sows. It differs greatly among pig breeds of different genetic backgrounds. Long non-coding RNAs (lncRNAs) can regulate follicle development, granulosa cell(GC) growth and hormone secretion, which in turn affects sow litter size.
Results: In our research, we identified 3554 lncRNAs and 25491 mRNAs in M2 follicle from Meishan and Duroc pigs. lncRNAs sequence and open reading frame(ORF) length is shorter than mRNAs, and it has fewer exons, lower abundance and conserved than protein-coding RNAs. Furthermore, 201 lncRNAs were differentially expressed in breeds, and quantitative trait loci (QTL) analysis of differential expression (DE) lncRNAs were performed. 127 DE lncRNAs are located in 119 reproduction trait-related loci. In addition, the lncRNAs potential target genes (PTGs) in cis or trans were predicted. Gene ontology(GO) and KEGG pathway analysis revealed that some PTGs were include some follicular development and hormone secretion-related biological processes or pathways, such as regulation of progesterone biosynthetic process, oestrogen metabolic process and ovarian steroidogenesis and PI3K-Akt signalling pathway. Furthermore，We also screened 19 differentially expressed lncRNAs of PI3K-Akt signalling pathway as candidates.
Conclusions: This study provided a new significance on the roles of lncRNAs in follicular growth and development and porcine reproduction.
Keywords
Duroc sows, Meishan sows, lncRNAs, Ovarian follicle, reproduction loci, PI3K-Akt
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Tao Huang
College of Animal Science and Technology, Shihezi University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2943-5131
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 05 Oct, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Ovulation rate is an extremely important factor of litter size in sows. It differs greatly among pig breeds of different genetic backgrounds. Long non-coding RNAs (lncRNAs) can regulate follicle development, granulosa cell(GC) growth and hormone secretion, which in turn affects sow litter size.
Results: In our research, we identified 3554 lncRNAs and 25491 mRNAs in M2 follicle from Meishan and Duroc pigs. lncRNAs sequence and open reading frame(ORF) length is shorter than mRNAs, and it has fewer exons, lower abundance and conserved than protein-coding RNAs. Furthermore, 201 lncRNAs were differentially expressed in breeds, and quantitative trait loci (QTL) analysis of differential expression (DE) lncRNAs were performed. 127 DE lncRNAs are located in 119 reproduction trait-related loci. In addition, the lncRNAs potential target genes (PTGs) in cis or trans were predicted. Gene ontology(GO) and KEGG pathway analysis revealed that some PTGs were include some follicular development and hormone secretion-related biological processes or pathways, such as regulation of progesterone biosynthetic process, oestrogen metabolic process and ovarian steroidogenesis and PI3K-Akt signalling pathway. Furthermore，We also screened 19 differentially expressed lncRNAs of PI3K-Akt signalling pathway as candidates.
Conclusions: This study provided a new significance on the roles of lncRNAs in follicular growth and development and porcine reproduction.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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