This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research Article
Coy Fuster, Pilar
University of Murcia
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3943-1890
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Declarations:
Declarations
- This study involved human subjects.
- The author confirmed that all appropriate ethical guidelines for the use of human subjects have been followed, any necessary IRB and/or ethics committee review has been obtained, and information about the IRB/ethics committee is included in the manuscript.
- The author has confirmed that all necessary patient/participant consent or assent has been obtained and the appropriate institutional forms have been archived. If the IRB/ethics committee waived the requirement for patient/participant consent or assent, an explanation for the waiver is included in the text.
- This study reports on a clinical trial.
- The author has confirmed that this trial has been registered with an ICMJE-approved registry, and the trial registration ID has been provided in the text. If the article reports on a study that was registered retrospectively, a statement explaining why the study was not registered in advance has been provided in the text.
- The author has confirmed that a statement listing potential conflicts of interest or lack thereof is included in the text.
Reproductive fluids have been recently proposed as the most adequate natural additive to complement the chemically defined in vitro culture media in different animal models, since they contain the full range of molecules necessary for the fertilization and development of preimplantation embryos. However, reproductive fluids potentially are problematic in terms of biosecurity and reproducibility of results, and their availability is scarce. Therefore, we have optimized the protocols to characterize the physico-chemical and functional properties of human reproductive fluids to establish the security ranges that could allow their use as natural additives and using the least possible volume of fluids collected. The results of this study offer, for the first time, a complete quality control guideline for human reproductive fluids, including the security levels (referred from physiological values) that should be taken into account for further use – i.e., osmolality, pH, total protein concentrations, presence of endotoxins and sterility conditions. In addition, a functional test has been developed evaluating the in vitro growth of bovine embryos in medium with human reproductive fluids. Finally, as a proof of concept, four embryos cultured with uterine fluid of their own mothers were transferred and two resulted in successful pregnancy and delivery of healthy babies. In conclusion, this study paves the way towards the future use of more natural culture media in human ART.
Keywords
Human reproductive fluids, culture media, quality control sheet, osmolality, pH, protein concentration.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 06 Aug, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Sexual & Reproductive Medicine
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Coy Fuster, Pilar
University of Murcia
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3943-1890
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 06 Aug, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Sexual & Reproductive Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Declarations:
Declarations
- This study involved human subjects.
- The author confirmed that all appropriate ethical guidelines for the use of human subjects have been followed, any necessary IRB and/or ethics committee review has been obtained, and information about the IRB/ethics committee is included in the manuscript.
- The author has confirmed that all necessary patient/participant consent or assent has been obtained and the appropriate institutional forms have been archived. If the IRB/ethics committee waived the requirement for patient/participant consent or assent, an explanation for the waiver is included in the text.
- This study reports on a clinical trial.
- The author has confirmed that this trial has been registered with an ICMJE-approved registry, and the trial registration ID has been provided in the text. If the article reports on a study that was registered retrospectively, a statement explaining why the study was not registered in advance has been provided in the text.
- The author has confirmed that a statement listing potential conflicts of interest or lack thereof is included in the text.
Reproductive fluids have been recently proposed as the most adequate natural additive to complement the chemically defined in vitro culture media in different animal models, since they contain the full range of molecules necessary for the fertilization and development of preimplantation embryos. However, reproductive fluids potentially are problematic in terms of biosecurity and reproducibility of results, and their availability is scarce. Therefore, we have optimized the protocols to characterize the physico-chemical and functional properties of human reproductive fluids to establish the security ranges that could allow their use as natural additives and using the least possible volume of fluids collected. The results of this study offer, for the first time, a complete quality control guideline for human reproductive fluids, including the security levels (referred from physiological values) that should be taken into account for further use – i.e., osmolality, pH, total protein concentrations, presence of endotoxins and sterility conditions. In addition, a functional test has been developed evaluating the in vitro growth of bovine embryos in medium with human reproductive fluids. Finally, as a proof of concept, four embryos cultured with uterine fluid of their own mothers were transferred and two resulted in successful pregnancy and delivery of healthy babies. In conclusion, this study paves the way towards the future use of more natural culture media in human ART.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Loading...