Evolution of the Angiopoietin-Like Gene Family and Their Role in Lipid Metabolism in Pigs

Background: Lipid metabolism is closely associated with various metabolic diseases, such as obesity, cardiovascular disease, diabetes, and cancer. More importantly, it also affects the carcass quality of animals. Angiopoietin-like protein (Angiopoietin-like, ANGPTL) family members are encoded by 8 genes and play an important role in lipid metabolism and angiogenesis. In this study, we rst systematically described the phylogenetic characteristics of pig ANGPTL family genes and identied the critical roles of ANGPTL3, ANGPTL4 and ANGPTL8 in the lipid metabolism of pigs. Methods: The amino acid sequence analysis, phylogenetic analysis, and chromosome adjacent gene analysis were performed to identify the ANGPTL gene family in pigs. Furthermore, according to the body weight data from 60 Jinhua pigs, different tissues of 6 pigs with average body weight were used to determine the expression prole of ANGPTL1-8. The ileum, subcutaneous fat, and liver of 8 pigs with distinct fatness were selected to analyze the gene expression of ANGPTL3, ANGPTL4, and ANGPTL8. Results: Results showed the sequence length of ANGPTLs in pigs was between 1186 and 1991 bp, and the pig ANGPTL family members shared common features with human homologous genes, including the high similarity of the amino acid sequence and chromosome anking genes. Amino acid sequence analysis showed that ANGPTL1-7 had a highly conserved domain except for ANGPTL8. Phylogenetic analysis showed that the ANGPTL homologues identied from 6 species could be divided into two categories. Each ANGPTL homologous gene was clustered with other mammalian sequences, away from other vertebrates. RT-qPCR analysis showed that ANGPTL family members had different expression patterns in different tissues. ANGPTL3 and ANGPTL8 were mainly expressed in the liver, while ANGPTL4 was expressed in many other tissues, such as the intestine and subcutaneous fat. The expression levels of ANGPTL3 and ANGPTL4 in Jinhua pigs with low propensity for adipogenesis were signicantly higher than those of high propensity for adipogenesis. Conclusions: These results increase our knowledge about the biological role of the ANGPTL


Introduction
The Angiopoietin-like protein (ANGPTL) family is a type of secretory protein that plays a variety of roles in lipid metabolism, glucose metabolism, energy metabolism, angiogenesis, and stem cell biology [1]. Excluding ANGPTL8, ANGPTL families are similar to Angiopoietins (ANG) and share common characteristics, including the N-terminal signal sequence (SS) and coiled-coil domain (CCD), that mediates the formation of homo-oligomers, and the C-terminal brinogen domain (FReD) that regulates ligand activity [2,3]. ANGPTL8, an atypical member of the ANGPTL family, consists of 198 amino acids and lacks FReD in humans [4,5]. Disordered lipid metabolism will lead to cardiovascular disease, diabetes, and obesity, and it also affects the meat quality of animals [6,7].
The investigation on improving meat production as well as meat quality has been pursued by many animal husbandry researchers. Extensive studies con rm that ANGPTL3, ANGPTL4, and ANGPTL8 play an important role in lipid metabolism and are all lipoprotein lipase (LPL) inhibitors [8,9]. LPL was originally identi ed as a triglyceride (TG) scavenging factor lipase [10]. It is produced by cardiomyocytes and adipocytes and is transported by GPIHBP1 protein to the lumen side of capillary endothelial cells [11], where it hydrolyzes TG into fatty acids for being absorbed into tissues [12,13].
ANGPTL3 is a liver-speci c secretion factor and is highly expressed in the liver [14]. Its expression is regulated by the liver X receptor (LXR) [15]. The main effect of ANGPTL3 is to inhibit the activity of LPL in the capillaries of adipose tissue and muscle [8,16]. Intriguingly, ANGPTL3 plays a major role in promoting the uptake of very low density lipoprotein (VLDL)derived TG into white adipose tissue (WAT) rather than oxidative tissues, such as skeletal muscle, heart brown adipose tissue in the feeding state [17].
ANGPTL4 is also known as fasting-induced adipokine (FIAF) or peroxisome proliferator-activated receptor gamma angiopoietin-related protein (PGAR) [18,19]. ANGPTL4 regulates lipid homeostasis and participates in regulating the intestinal microbiota in fat deposition [20,21]. Unlike ANGPTL3, ANGPTL4 can be secreted by adipose tissue, intestinal tissues, liver, skeletal muscle, heart, and other tissues [22]. ANGPTL4 is released as a 50 kD hormone precursor, which is then cut into N-terminal and C-terminal fragments [13,23]. The N-terminus of ANGPTL4 acts as a lipoprotein lipase (LPL) inhibitor [22]. Research shows that ANGPTL4 is negatively correlated with low-density lipoprotein (LDL) cholesterol and high-density lipoprotein (HDL) cholesterol and is positively correlated with various lipid metabolic syndromes [24]. The overexpression of ANGPTL4 increases plasma TG levels [25]. ANGPTL4 is also an endogenous inhibitor to intestinal fatty digestive enzymes. The knockout of ANGPTL4 in mice would increase the body weight, decreased the lipid content in feces, and increased dietary triglyceride accumulation in the small intestine, which was consistent with the increased intestinal lumen lipase activity [21,26]. ANGPTL3 mainly inhibits LPL activity in the feeding state, whereas ANGPTL4 plays an important role in both feeding and fasting states [1]. Different from ANGPTL3, ANGPTL4 is considered to be an endocrine or autocrine/paracrine inhibitor of LPL due to its different expression sites [27].
ANGPTL8 is a new protein mainly expressed in the human liver and plays an important role in regulation of plasma TG levels and lipid metabolism [28]. In addition, the N-terminal domain of ANGPTL8 is 20% identical to ANGPTL3 and ANGPTL4 and functionally binds to LPL to regulate triglyceride metabolism, indicating the a nity between them [29,30]. ANGPTL8 could interact with ANGPTL3, and they could synergistically inhibit LPL activity and regulate the plasma triglyceride levels [31,32]. The overexpression of ANGPTL8 in the liver of ANGPTL3 knockout mice failed to increase the levels of triacylglycerol nonesteri ed fatty acids in plasma [33]. Since ANGPTL3, ANGPTL4, and ANGPTL8 are all LPL inhibitors, the absence of anyone of them would break the balance of triglyceride metabolism in turn would lead to hypotriglyceridemia or hypertriglyceridemia. During fed-fast cycle, feeding could induce the expression of ANGPTL8 and activate the interaction between ANGPTL8 and ANGPTL3 in mice, thereby inhibiting LPL activity in the myocardium and bones. Conversely, fasting could inhibit the expression of ANGPTL8 and improve the expression of ANGPTL4 in mice, resulting the transportation of TG to muscles [34].
So far, studies on the ANGPTLs have focused on mice [35] and humans [36], but reports on the ANGPTL gene in domestic animals are limited. The Jinhua pig is an important breed in China with a high percentage of body fat and good meat quality. Pigs are more closely related to humans in carbohydrate and lipid metabolism than mice [37]. Compared to mice, pigs are considered to be more suitable to be developed as animal models for studying human obesity and fat metabolism [38,39]. In this study, we reported the basic information of the ANGPTL1-8 gene in Jinhua pigs, the evolutionary relationship between the pig ANGPTL1-8 gene and other different vertebrates, their expression pro le in different tissues, and the differences in the mRNA expression levels of ANGPTL3, ANGPTL4, and ANGPTL8 between obese and lean Jinhua pigs. The purpose of this study is to establish a theoretical foundation for the roles of the ANGPTL family genes in the fat regulation of pigs.

Data Mining and Gene Identi cation
The genome of pig ANGPTL family members was retrieved from the NCBI database to obtain basic information, such as transcript ID and mRNA length(bp) [40]. The relative molecular weights and charge was performed using the programs within the DNASTAR Lasergene [41], the ClustalW was used to generate the amino acid sequences [42]. Exon-intron boundaries and chromosomal locations were identi ed by the mRNA-genome alignment program Spidey [43].

Phylogenetic and Evolutionary Analyses
The MUSCLE program was used to align amino acid sequences (obtained from the NCBI database), and Boxhade software was used for visual display. Phylogenetic analysis of the ANGPTL family members in humans and animals, including mice, cattle, pigs, dogs, and chickens, was carried out using the deduced mature protein sequences. The Mega7.0 software was used to construct phylogenetic trees from P-distances of ANGPTL amino acid sequences from pigs and other representative vertebrates [44]. The Bootstrap method was used to test the reliability of each branch 1000 times.

Short-range gene linkage
In order to further con rm the homology of the ANGPTL gene and establish the evolutionary model of the ANGPTL gene in metazoa, we isolated the genetic environment of ANGPTL3 and ANGPTL4 chromosomes or genomic fragments of vertebrates to determine whether there are homologous genomic regions in vertebrates. Similarly, the genetic environment of mammalian ANGPTL8 is described. The short-range gene linkage comparison was performed among humans, mice, cattle, pigs, dogs, and poultry. The adjacent genetic environment of vertebrates was retrieved from the Genomicus database, and the genetic environment of human ANGPTL3, ANGPTL4, and ANGPTL8 were used as a reference sequence [45]. Homologous genomic regions were characterized by the discovery of speci c genomic combinations of conserved anking genes.

Animals and Sampling
A total of 60 Jinhua Pigs at 90-day old were obtained from the experimental pig farm of Jinhua Academy of Agricultural Sciences (Jinhua, Zhejiang Province, China). All Jinhua pigs were reared in pens (10 pens, 6 pigs per pen) in an environmentally controlled facility and had free access to commercial diets and water under a standard management. At 270 days of age, pigs were weighed invidually and slaughtered. Backfat thickness was measured. Based on the body weight data, different tissues were collected from 6 pigs with average body weight, immediately frozen in liquid nitrogen, and stored in a -80°C freezer until RNA isolation. The tissues included tongue, esophagus, stomach, duodenum, jejunum, ileum, cecum, colon, trachea, lung, heart, longissimus muscle, subcutaneous fat, liver, kidney, brain, pancreas, and spleen. Eight of 92.35 ± 10.10 kg pigs with the higher and lower backfat thickness were set as the high group (H group) and low group (L group), respectively [46]. The ileum, subcutaneous fat, and liver segments of the H and L group pigs were collected, immediately frozen in liquid nitrogen, and stored in a -80°C freezer until RNA isolation.

RNA Extraction and Real-Time Quantitative PCR
According to the manufacturer's instructions, total RNA was extracted from each tissue sample using a TRI-zol Plus RNA Puri cation Kit (Invitrogen, Carlsbad, CA). RNA integrity and purity were assessed by Nanodrop after being electrophoresed in a formaldehyde gel [47,48]. The genomic DNA contamination was removed from the RNA samples using a gDNA Eraser (Takara Bio Inc., Dalian, China), and the PrimeScript RT reagent kit (Takara Bio Inc.) was subsequently used according to the manufacturer's instructions for reverse transcription. The cDNA was then diluted ten times with RNase-free water before RT-qPCR analysis. RT-qPCR was performed on each sample by using the CFX96 RT-qPCR Detection System (Bio-Rad Laboratories, Richmond, VA), and the ampli cation was conducted in a total volume of 20 µl, containing 10 µl of SYBR Premix Ex Taq II (Takara Bio Inc.), 7 µl of RNase-free water, 1 µl of the diluted cDNA, and 0.5 µl of each primer ( Table 1). The optimum RT-qPCR program was 95˚C for 1 min, followed by 40 cycles of 95℃ for 15s and 60℃ for 25s. Relative quanti cation of the mRNA transcripts was accomplished using the 2 −ΔΔCT method and the housekeeping gene was glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

Results
Basic Information of Pig ANGPTL1-8 The nucleotide sequences of pig ANGPTL1-8 were obtained from the NCBI database, and the basic information was generated by DNASTAR Lasergene (Table 1). The mRNA sequences of pig ANGPTL1-8 were between 1100 and 2000 bp in length; the numbers of amino acid residues of ANGPTL1-7 were between 344-493 while ANGPTL8 was 198. ANGPTL1-8 was composed of 4-9 exons determined by Spidy program analysis (Table 1). Sequence Conservation of Pig ANGPTLs Multiple sequence alignment of amino acid sequences of pig ANGPTL1-8 showed that the amino acid sequence of ANGPTL1-7 was highly conserved, and the amino acid sequence of ANGPTL8 was less conserved (Fig. 1)

Phylogeny of the Pig ANGPTLs
Phylogenetic analysis of the vertebrate ANGPTL family suggested that these genes shared a common origin, and the family members emerged early during metazoan radiation (Fig. 2). According to the dendritic topology, there are ve main protein clusters that contain different members of the ANGPTL family: the ANGPTL1-2-6 cluster (ANGPTL1, ANGPTL2, ANGPTL6), the ANGPTL3-4 cluster (ANGPTL3, ANGPTL4), the ANGPTL5 cluster, the ANGPTL7 cluster, and the ANGPTL8 cluster, which was isolated from the other four protein clusters (Fig. 2). Each ANGPTL orthologous gene was clustered with other mammalian sequences and separately from poultry.

Neighbouring Gene Analysis
By analyzing and comparing the genetic environment of ANGPTL3 (Fig. 3) and ANGPTL4 (Fig. 4) in mammals and poultry, we could further understand the evolutionary relationship of ANGPTL family genes in vertebrate radiation. The pig ANGPTL3 gene was mapped to chromosome 6, and the ANGPTL4 gene was mapped to chromosome 2 and was also found to be present in other mammals and chickens. Among the ten genes anking human ANGPTL3, nine were retained on pig chromosome 6, and seven were retained on chicken chromosome 8. Of the ten genes anking human ANGPTL4, nine were retained on pig chromosome 2, and nine were retained on chicken chromosome 28. This indicated that the genetic environment of several major mammals (humans, mice, cattle, pigs, and dogs) ANGPTL3 was highly similar and was also similar to that of chicken ANGPTL3, indicating that mammals and poultry ANGPTL3 and ANGPTL4 shared a common ancestral origin. Comparing the adjacent genetic environment of mammalian ANGPTL8 (Fig. 5) in pigs, ANGPTL8 was located on chromosome 2. Among the ten genes anking human ANGPTL8, all were retained on cow chromosome 7, and nine genes were retained on pig chromosome 2. This indicated that the genetic environment of ANGPTL8 in several major mammals (humans, mice, cattle, pigs, dogs) were highly similar, which means that the mammalian ANGPTL8 had a common ancestral origin.

Gene expression pattern of pig ANGPTL family
In order to study the expression patterns of the ANGPTL gene family in pigs, the mRNA abundance of ANGPTL1-8 in 18 tissues of 6 Jinhua pigs was determined by RT-qPCR. As shown in Fig. 6, ANGPTL4 was widely expressed in various tissues, while ANGPTL3 and ANGPTL8 were predominantly expressed in the liver, and ANGPTL8 was only expressed in the liver. Meanwhile, it was observed that ANGPTL4 was highly expressed in the intestinal tissues with relative low expression in subcutaneous fat, lung, heart, and longissimus muscle. ANGPTL4 relative expression level was the highest in subcutaneous fat.
Expression levels of ANGPTL3, ANGPTL4 and ANGPTL8 in Jinhua pigs with distinct fatness To con rm whether lipid metabolism was associated with the expression of ANGPTL3, ANGPTL4, and ANGPTL8, we selected six Jinhua pigs with average body weight for the determination of body weight, backfat thickness, and expression level of ANGPTL3, ANGPTL4, and ANGPTL8 by RT-qPCR analysis following RNA isolation and reverse transcription. The backfat thickness of Jinhua pigs in the H group was signi cantly higher than that of Jinhua pigs in the L group (Fig. 7). However, there were no signi cant differences in the body weight of Jinhua pigs between the H and L groups (Fig. 7). The relative expression of ANGPTL3 in the liver of the H group pigs was signi cantly lower than that of the L group pigs (P = 0.005) while the relative expression levels of ANGPTL4 in the ileum (P = 0.043), subcutaneous fat (P = 0.025), and liver (P = 0.008) of the H group pigs were lower than those of the L group pigs (Fig. 8). However, the relative expression of ANGPTL8 in the liver of the H group pigs was not signi cantly different from that of the L group pigs (P > 0.05).

Discussion
The Jinhua pig has become one of the most important local breeds in China due to its excellent meat quality and it exhibits the characteristics of early sexual maturity, low proli cacy, and a high body fat content [50].The purpose of this study was to explore the evolutionary relationship of ANGPTL family genes in Jinhua Pig, a model animal of lipid metabolism, and the important roles of ANGPTL3, ANGPTL4 and angptl8 in lipid metabolism. In the present study, amino acid sequence analysis, phylogenetic analysis, chromosome adjacent gene analysis, and RT-qPCR analysis showed that Jinhua pig ANGPTL family members had common characteristics with homologous human genes, including the high similarity of amino acid sequence and chromosome anking genes. Consistent with earlier studies [51,52], pig ANGPTL3-4 had typical angiopoietin family structures. The structures of pig ANGPTL3-4 was similar to those of humans and mice, and both had FReD, which affects angiogenic activity, and CCD, which regulated fat metabolism by inhibiting LPL. The eight ANGPTL family member genes in Jinhua pigs were 1100-2000 bp in length and consisted of 4-9 exons. The functional helical domain and brinogen-like domain in the ANGPTL1-8 amino acid sequence were strongly conserved. Through the identi cation and expression analysis of the bovine ANGPTL1-7 genes, all of the deduced amino acid sequences had an N-terminal crimped domain and a C-terminal brinogen-like domain, and both had the characteristics of angiogenin. The bovine ANGPTL1-7 amino acid sequences are similar to those of humans, and the corresponding members had 95%, 97%, 82%, 85%, 87%, 85%, and 87% homology [43]. Related studies reported the cloning, chromosomal location, and expression analysis of pig ANGPTL3 and ANGPTL4. In vertebrates, the ANGPTL family of genes evolved by gene duplication, gene deletion, and gene mutation. They share the same evolutionary origin with angiogenin and share similarities in sequence and structure. ANGPTL family members expanded in early vertebrate genome doubling and genome segment replication before vertebrate radiation. Five major ANGPTL vertebrate protein clusters, ANGPTL1-2-6, ANGPTL3-4, ANGPTL5, ANGPTL7, ANGPTL8, derived from the replication of ancestor ANGPTL [53,54]. The ANGPTL8 cluster was isolated from the other four protein clusters since ANGPTL8 has a different structure from other ANGPTL family members [33]. Clustering in phylogenetic trees indicates that pig sequences are not signi cantly different from those of other vertebrates.
Recent studies have shown that ANGPTL3 [55], ANGPTL4 [56], and ANGPTL8 [57] are directly related to lipid metabolism and fat deposition in the body. In this study, tissue expression pro les showed that ANGPTL3 and ANGPTL8 were mainly expressed in the liver of pigs, with similar results in humans and mice [58,59]. However, ANGPTL4 in pigs is widely expressed in many tissues, including the gastrointestinal tract, subcutaneous fat, liver, muscles, heart, and lungs. In humans and mice, ANGPTL4 is also expressed mainly in white adipose tissue and the liver as well as in the intestine and heart tissue [18, 60]. By analyzing the expression levels of ANGPTL3 and ANGPTL8 in the liver and the relative expression levels of ANGPTL4 in the liver, subcutaneous fat, and ileum of Jinhua pigs with distinct fatness, we found that the expression of ANGPTL3 in the liver of Jinhua pigs in the H group was signi cantly lower than that of Jinhua pigs in the L group (P = 0.005). Moreover, the expression level of ANGPTL4 in the liver (P = 0.008), subcutaneous fat (P = 0.025), and ileum (P = 0.043) of the H group pigs was signi cantly lower than that of the L group pigs. This might be due to the inhibitory effect of ANGPTL3 and ANGPTL4 on LPL. With the decrease in the expressions of ANGPTL3 and ANGPTL4, the activity of LPL would increase, which in turn would decrease the serum TG level and increase the lipid absorption. Early research showed that in sterile mice with routine feeding, the microbiota promoted the storage of triglycerides in fat cells by inhibiting the intestinal expression of ANGPTL4, illustrating the decrease of ANGPTL4 expression is bene cial to fat storage [20]. Recent studies have indicated that the level of circulating ANGPTL4 in obese children was lower than that of normal weight children. Waist circumference, body weight, and other major obesity indicators were negatively correlated with the level of circulating ANGPTL4 [61]. Meanwhile, mice with ANGPTL3 and ANGPTL4 de ciency had severe hypertriglyceridemia while mice with ANGPTL3 overexpression had hyperlipidemia [62,63]. Furthermore, compared with other breeds of pigs, the fat percentage of Large White pigs was lower with a higher expression of ANGPTL4 mRNA [64]. These studies are consistent with the results of the present study.
Although there was no signi cant difference in ANGPTL8 expression between the liver of Jinhua pigs in the H and L groups, it might be resulted from the function of ANGPTL8, which promotes the cleavage of ANGPTL3 and binds to the N-terminal of ANGPTL3 [34]. The interaction of ANGPTL8 with ANGPTL3 would form a complex with the N-terminal of ANGPTL3, synergistically inhibiting the LPL activity and modulating the plasma triglyceride levels [31]. Additionally, the overexpression of ANGPTL8 in the liver of ANPTL3 gene knockout mice had no effect on the triglyceride metabolism [33].
Fat metabolism and faat storage are closely related to the meat quality and carcass quality. Relevant research showed that by comparing the carcass composition and development patterns of Jinhua and Landrace pigs at 35 ~ 125 days of age, the carcass fat content of Jinhua pigs was signi cantly higher than that of Landrace pigs (P < 0.05), and the lean meat rate of the carcass was signi cantly lower than that of Landrace pigs (P < 0.01) [65]. Similar studies showed that Jinhua pigs had a higher tendency to deposit fat compared with Landrace pigs [39]. These are consistent with the results of this experiment. Therefore, Jinhua Pigs with high body fat percentage and good meat quality play an important role in the study of lipid metabolism and carcass quality.

Conclusion
Collectively, the present study identi ed ANGPTL family members in Jinhua pigs. The expression of ANGPTLs was tissue-speci c in Jinhua pigs. Moreover, the expression levels of ANGPTL3 and ANGPTL4 were associated with fat metabolism in Jinhua pigs. This study would enrich our knowledge of the pig ANGPTL gene family and con rm the importance of ANGPTL3 and ANGPTL4 in regulating the lipid metabolism and improving the carcass quality of pigs. Availability of data and materials The datasets reported in this manuscript are available from the corresponding author on reasonable request.
Ethics approval and consent to participate All animals used in this study were reviewed and approved by the Institutional Animal Care and Use Committee of Zhejiang Academy of Agricultural Sciences. Written informed consent was obtained from the owners for the participation of their animals in this study.

Consent for publication
The authors declare consent for publication.

Figure 1
Multiple sequence alignment of representative pig ANGPTL protein sequences. The conserved residues are shaded. Speci cally, the black part is highly similar, the gray part is less similar, and the non-color covered part has no similarity.

Figure 2
Phylogenetic analysis of pig ANGPTL genes and their representative sequences from human and animals. The topology was constructed using the amino acid sequences by the Neighbor-Joining method with 1000 bootstrap repeats. Only the branches with a bootstrap value greater than 50 are displayed at branching points.  Comparison of homologous genomic regions of pig ANGPTL4 with humans, mice, and several other animals. The genetic environment of the pig ANGPTL4 gene was characterized, and the homologous genes of humans, mice, and other animals were identi ed. The horizontal line represents the chromosome segment, the arrow box represents the gene, and the arrowhead points in the direction of predicted gene transcription. Only genes preserved across species will appear. The same color is of the gene homologs, and they are presented according to their order in the chromosome. The gene names and symbols are: ceramide synthase 4 (CERS4), CD320 molecule (CD320), NADH:ubiquinone oxidoreductase subunit A7 (NDUFA7), Ribosomal protein S28 (RPS28), KN motif, and ankyrin repeat domains 3 (KANK3), Angiopoietin-like 4 (ANGPTL4), RAB11B, member RAS oncogene family (Rab11B), Membrane-associated ring nger (C3HC4) 2 (MARCH2), Heterogeneous nuclear ribonucleoprotein M (HNRNPM), PML-RARA regulated adaptor molecule 1 (PRAM1), Zinc nger protein 414 (ZNF414).

Figure 5
Comparison of homologous genomic regions of pig ANGPTL8 with humans, mice, and several other animals. The genetic environment of the pig ANGPTL8 gene was characterized, and the homologous genes of humans, mice, and other animals were identi ed. The horizontal line represents the chromosome segment, the arrow box represents the gene, and the arrowhead points in the direction of predicted gene transcription. Only genes preserved across species will appear. The same color is of the gene homologs, and they are presented according to their order in the chromosome. The gene names and symbols are: SWI/SNF related, matrix associated, actin-dependent regulator of chromatin, subfamily a,  Relative expression of ANGPTL1-8 in different tissues, including tongue, esophagus, stomach, duodenum, jejunum, ileum, cecum, colon, trachea, lung, heart, longissimus muscle, subcutaneous fat, liver, kidney, brain, pancreas, and spleen of Jinhua pigs. The indicated tissue segments were collected from 6 Jinhua pigs at 270-day old following by RNA isolation, reverse transcription and RT-qPCR. Data was expressed as mean ± SEM (n = 6).

Figure 7
Body weight (A) and backfat thickness (B) of Jinhua pigs in the H and L groups. Jinhua pigs with similar body weight and signi cantly different backfat thickness were weighed individually and the backfat thickness of each pig was measured at 270-day old. H: the H group was consisted of pigs with relative high backfat thickness; L: the L group was consisted of pigs with relative low backfat thickness. Data was expressed as mean ± SEM (n = 8) and analyzed by oneway ANOVA analysis followed by an unpaired two-tailed Student's t-test.

Figure 8
The expression of ANGPTL3, ANGPTL4, and ANGPTL8 in the liver (A, B, and C), subcutaneous fat (D), and ileum (E) of Jinhua pigs in the H and L groups. The liver, subcutaneous fat, and ileum segments were collected from 16 Jinhua pigs at 270-day old following by RNA isolation, reverse transcription and RT-qPCR. H: the H group was consisted of pigs with relative high backfat thickness; L: the L group was consisted of pigs with relative low backfat thickness. Data was expressed as mean ± SEM (n = 8) and analyzed by one-way ANOVA analysis followed by an unpaired two-tailed Student's t-test.