This preprint is under consideration at BMC Pregnancy and Childbirth. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
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Research article
Jiaman Wu
Shenzhen Maternity&Child Healthcare Hospital
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Premature ovarian insufficiency (POI) is characterized by impairment of ovarian function on a continuum before the age of 40 years. POI is affected by multiple factors. Considering new insights from recent gut microbiome studies, this study aimed to investigate the relationship between gut microbial community structure and POI. Subjects were recruited at the Shenzhen Maternity&Child Healthcare Hospital. Fecal microbial community profiles of healthy women (n = 18), women with POI (n = 35) were analyzed using 16S rRNA gene sequencing based on Illumina NovaSeq platform. Compared to the controls, the serum levels of FSH, LH, T and FSH/LH ratio significantly increased in women with POI, whereas E2 and AMH decreased significantly. Higher weighted UniFrac value was observed in POI women compared with healthy women. Phylum Firmicutes, genera Bulleidia and Faecalibacterium were more abundant in healthy women, while phylum Bacteroidetes, genera Butyricimonas, Dorea, Lachnobacterium and Sutterella enriched significantly in women with POI. Moreover, these alterations of the gut microbiome in women with POI was closely related to FSH, LH, E2, AMH level and FSH/LH ratio. Women with POI had altered microbial profiles in their gut microbiome, which were associated with serum hormones levels. These results will shed a new light on the pathogenesis and treatment for POI.
Keywords
premature ovarian insufficiency, POI, gut microbiota, 16S rRNA sequencing
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CURRENT STATUS: Under Review
Version 1
Posted 12 Aug, 2020
Community comments: 1
Editorial decision: Major revision
On 30 Jan, 2021
Review #3 received
Received 28 Jan, 2021
Reviewer #3 agreed
On 20 Dec, 2020
Review #2 received
Received 07 Dec, 2020
Reviewer #2 agreed
On 27 Nov, 2020
Review #1 received
Received 06 Nov, 2020
Reviewer #1 agreed
On 17 Oct, 2020
Reviewers invited
Invitations sent on 21 Aug, 2020
Editor assigned
On 06 Aug, 2020
First submitted
On 05 Aug, 2020
Submission checks complete
On 05 Aug, 2020
Editor invited
On 05 Aug, 2020
Metrics
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HTML Views: ...
Subject Areas
Maternal & Fetal Medicine
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This preprint is under consideration at BMC Pregnancy and Childbirth. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Jiaman Wu
Shenzhen Maternity&Child Healthcare Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 12 Aug, 2020
Community comments: 1
Editorial decision: Major revision
On 30 Jan, 2021
Review #3 received
Received 28 Jan, 2021
Reviewer #3 agreed
On 20 Dec, 2020
Review #2 received
Received 07 Dec, 2020
Reviewer #2 agreed
On 27 Nov, 2020
Review #1 received
Received 06 Nov, 2020
Reviewer #1 agreed
On 17 Oct, 2020
Reviewers invited
Invitations sent on 21 Aug, 2020
Editor assigned
On 06 Aug, 2020
First submitted
On 05 Aug, 2020
Submission checks complete
On 05 Aug, 2020
Editor invited
On 05 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Maternal & Fetal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Premature ovarian insufficiency (POI) is characterized by impairment of ovarian function on a continuum before the age of 40 years. POI is affected by multiple factors. Considering new insights from recent gut microbiome studies, this study aimed to investigate the relationship between gut microbial community structure and POI. Subjects were recruited at the Shenzhen Maternity&Child Healthcare Hospital. Fecal microbial community profiles of healthy women (n = 18), women with POI (n = 35) were analyzed using 16S rRNA gene sequencing based on Illumina NovaSeq platform. Compared to the controls, the serum levels of FSH, LH, T and FSH/LH ratio significantly increased in women with POI, whereas E2 and AMH decreased significantly. Higher weighted UniFrac value was observed in POI women compared with healthy women. Phylum Firmicutes, genera Bulleidia and Faecalibacterium were more abundant in healthy women, while phylum Bacteroidetes, genera Butyricimonas, Dorea, Lachnobacterium and Sutterella enriched significantly in women with POI. Moreover, these alterations of the gut microbiome in women with POI was closely related to FSH, LH, E2, AMH level and FSH/LH ratio. Women with POI had altered microbial profiles in their gut microbiome, which were associated with serum hormones levels. These results will shed a new light on the pathogenesis and treatment for POI.
Figure 1
Figure 2
Figure 3
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