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Metal tolerance proteins (MTP) encompass plant membrane divalent cation transporters to specifically participate in heavy metal stress resistance and minerals acquisition. However, the molecular behaviors and biological functions of this family in M. truncatula are scarcely known. We identified 12 potential MTP candidate genes and analyzed for a phylogenetic relationship, chromosomal distributions, gene structures, protein structures, gene ontology, and previous RNA-seq data. MtMTPs were classified into three major cation diffusion facilitator (CDFs) groups; Mn-CDFs, Zn-CDFs, and Fe/Zn-CDFs. Structural analysis of SlMTPs displayed high gene similarity within the same group where all of them have cation_efflux domain or ZT_dimer. RNA-seq and gene ontology analysis revealed a significant role of MTP genes during M. truncatula growth and development.MTP genes showed tissue-specific and variable expression levels under the stress of the following five divalent heavy metals (Cd2+, Co2+, Mn2+, Zn2+, and Fe2+). Expression levels of Fe2+/MtMTP11 and Mn2+/MtMTP4 were upregulated, while Mn2+/MtMTP5 was downregulated. In conclusion, MtMTP1.1, MtMTP1.2, and MtMTP4 play a key role under heat and heavy metal stress in M. truncatula.
Keywords
Medicago truncatula, heavy metals, metal tolerance protein (MTP),genome-wide identification, gene expression.
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CURRENT STATUS: Under Review
Version 1
Posted 08 Jun, 2021
Community comments: 1
Reviews received
Received 06 Jun, 2021
Reviewers invited
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Editor invited
On 02 Jun, 2021
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On 24 May, 2021
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This preprint is under consideration at Environmental Science and Pollution Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 08 Jun, 2021
Community comments: 1
Reviews received
Received 06 Jun, 2021
Reviewers invited
Invitations sent on 05 Jun, 2021
Editor invited
On 02 Jun, 2021
Editor assigned
On 24 May, 2021
First submitted
On 19 May, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Metal tolerance proteins (MTP) encompass plant membrane divalent cation transporters to specifically participate in heavy metal stress resistance and minerals acquisition. However, the molecular behaviors and biological functions of this family in M. truncatula are scarcely known. We identified 12 potential MTP candidate genes and analyzed for a phylogenetic relationship, chromosomal distributions, gene structures, protein structures, gene ontology, and previous RNA-seq data. MtMTPs were classified into three major cation diffusion facilitator (CDFs) groups; Mn-CDFs, Zn-CDFs, and Fe/Zn-CDFs. Structural analysis of SlMTPs displayed high gene similarity within the same group where all of them have cation_efflux domain or ZT_dimer. RNA-seq and gene ontology analysis revealed a significant role of MTP genes during M. truncatula growth and development.MTP genes showed tissue-specific and variable expression levels under the stress of the following five divalent heavy metals (Cd2+, Co2+, Mn2+, Zn2+, and Fe2+). Expression levels of Fe2+/MtMTP11 and Mn2+/MtMTP4 were upregulated, while Mn2+/MtMTP5 was downregulated. In conclusion, MtMTP1.1, MtMTP1.2, and MtMTP4 play a key role under heat and heavy metal stress in M. truncatula.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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