DOI: https://doi.org/10.21203/rs.2.14759/v1
Traditionally a disease of the elderly, colorectal cancer (CRC) incidence in the young is steadily rising across the globe(1,2). In contrast, the incidence of CRC in older patients is seeing a progressive decrease in the developed world, which is likely to be attributed to population-based CRC screening(3–5). CRC is the third most common cancer worldwide, with approximately 10% of cases affecting patients aged under 55 years(4,6). These younger patients often present with more advanced disease and adverse pathological features compared to their older counterparts(7). This may have a negative impact on their survival outcome(8).
Evidence regarding young-onset CRC (yCRC) patient prognosis is conflicting. Some retrospective studies suggest that younger patients have a poorer prognosis than those with later-onset disease(8). However, other studies suggest their prognosis is better or equivalent to those aged over 55 years(9).
Inherited predispositions to CRC are sometimes responsible for causing the disease, especially within the younger demographic(8). These predispositions can be divided into low-penetrance familial clusterings and high-penetrance autosomal dominant cancer syndromes(3). The former carry a low associated risk and – at present – have no identifiable genetic cause(3). The latter are defined by the presence of microsatellite instability (MSI) and include Lynch syndrome and familial adenomatous polyposis (FAP)(3,8). Other causes of CRC are deemed sporadic and do not carry a genetic link. The population prevalence of Lynch syndrome has been estimated to be as high as 1:200 in some studies(10), with an associated lifetime risk of developing CRC of 50-70% as well as an increased risk of endometrial, ovarian and urothelial malignancies(11). Diagnosing Lynch syndrome – rather than sporadic CRC – has serious implications regarding a patient’s management and family screening(12)(10).
Given the relatively limited and conflicting data regarding this expanding subgroup of yCRC patients, the primary aim of this study was to determine the survival outcomes and clinico-pathological features of CRC patients aged under 55 years in the North East of Scotland. During the time period of this study, the Scottish Intercollegiate Guidelines Network (SIGN) criteria were used by the healthcare team in Aberdeen Royal Infirmary to decide whether a patient should receive a genetic test based on their age and family history(13)(14). Considering the growing relevance of genetic results in yCRC patients’ management, the secondary aim of this study was to determine the referral rate to genetic services and to establish the incidence of MSI in this patient subgroup.
Patients were initially identified using pathology records held at Aberdeen Royal Infirmary (ARI). The population included patients from Aberdeen City, Aberdeenshire, Orkney, Shetland and Moray. Inclusion criteria for the study were a diagnosis of CRC between 2005-2015 and aged between 18-55 years old at the time of diagnosis. Patients with unavailable or insufficient notes and those who did not fit the pre-specified inclusion criteria were excluded from the study.
Following registration with and approval of the study by NHS Grampian Clinical Audit Unit, data was extracted retrospectively from the NHS Grampian general and genetic patient records, using the electronic case record supplemented by paper files when required. De-identified data on patient characteristics (age at the time of diagnosis, year of diagnosis, sex, presentation and relevant past medical history); family history (including genetic referral, referral source and the outcome of referral); pathological details of tumour (location, TNM staging, Dukes staging, tumour differentiation and genetic markers of tumour); treatment approach; genetic care (referred to genetics services, seen in clinic, undergone genetic testing and the associated result) and survival (disease recurrence, patient deaths and time to death) were extracted.
Data was analysed using SPSS statistics version 25 (IBM, New York, USA). Kaplan-Meier estimates were used to evaluate survival and also compare survival outcomes between age groups of CRC, presentation type, chemotherapy agent, sex, tumour differentiation, Dukes’ staging, year of diagnosis and micro- satellite instability (MSI) status. The log rank test was used to evaluate results, with p<0.05 deemed as significant. Descriptive statistics for age, sex, stage, site of cancer, co-morbidities and treatment received were initially expressed as median and interquartile range and percentages.
Genetic referral was assessed according to SIGN guidelines in use when the cohort began (2005). Thus, the prior guidelines from 2003 were used rather than those used currently in clinical practice i.e.
Population
Between 03/01/2005 and 22/12/2015, 418 patients aged under 55 years had a primary colorectal cancer identified from the NHS Grampian pathology record. 73 patients were excluded, therefore the final number included in the study was 345. Age ranged from 22 to 54, with a mean age of 47.7 years (SD±6.1 years).
Survival
Death attributed to CRC occurred in one third of patients. Mean overall survival for CRC under 55 years was 96.1 months (95% confidence interval [CI], 89.2-102.9 months). In the univariate analysis, the following factors were associated with longer survival: increased age, Dukes’ A or B staging, presentation via screening and male sex. The survival plots are shown in figure 1 and the corresponding survival data is detailed in table 2. Five year survival rates in those aged less than 40 years was found to be worse (57%) compared to those aged between 40 and 54 years (68% and 62%).
Clinico-pathological characteristics
In patients with yCRC, disease presented symptomatically in 60.0%, as an emergency in 22.0% and incidentally in 0.6% of patients. Only 30.0% of the patients aged 50-55 years were identified through the bowel screening programme. A small number of patients (4.0%) had a past medical history of inflammatory bowel disease (IBD). Similarly, 4.0% had had an unrelated previous cancer such as testicular, vulval, breast or kidney cancer. One patient had had a previous CRC and only one patient was noted to have been a life-long vegetarian. The distribution of Dukes’ staging A-D were 12%, 26%, 43% and 19%, respectively. In patients aged under 40 years, 75.0% presented with advanced disease – i.e. Dukes’ C or D staging. This is a greater proportion compared to patients aged between 40 and 54 years where 59.3% presented with Dukes’ C or D disease. A majority of tumours (43.1%) were located in the rectum and 3.5% of the cohort were identified to have synchronous disease. Histologically, tumour differentiation was reported as “well”, “moderate” and “poor” in 1.2%, 78.6% and 12.2% of patients, respectively. Patients deemed incurable at diagnosis (10.7%) did not receive surgical or endoscopic tumour resection due to late stage disease. Adjuvant or neo-adjuvant chemotherapy was administered to 81.3% and 34.1% had received radiotherapy. Cancer recurrence occurred in 28.2% of patients and 33.4% died of CRC. Epidemiological and clinicopathological characteristics of the patients by age are displayed in table 3.
Genetic Referral
Family history (FH) was clearly documented in the general case record notes of 185 (53.6%) patients. A positive FH for CRC was reported in 91 (26.4%) patients, of which 53 were first-degree. FH of associated Lynch syndrome tumours were also documented where possible; endometrial cancer and breast cancer FH was positive in 10 (2.9%) and 25 (7.2%) patients, respectively. SIGN guidelines (table 1) were used to identify those requiring genetic referral. All cases aged under 50 years required referral – 172 patients (49.9%) – and those also with a first-degree FH of Lynch-related tumours – 88 patients (25.5%) – therefore qualified. Where positive FH had been documented but details of age or affected family member were unclear, cases were categorised into referral required (4 cases). In total, 201 (58.3%) of the cohort required genetic referral. Of these, 93 had such referral documented, however 108 patients who fulfilled the SIGN criteria to receive genetic testing for MSI were not referred to genetic services. An additional 18 patients were referred that did not require a genetics referral based on age or family history according to the SIGN 2003 guidelines. However, 28 of those referred did not get tested. Figure 2 demonstrates the referral of patients to genetic services. Of the 83 tested, 12 patients (14.5%) were identified to have Lynch syndrome and 3 patients (3.6%) identified to carry FAP.
As outlined in figure 2, a total of 111 patients (32.2% of cohort) were referred to genetics services. A further 28 were also referred to genetics but not seen; possibly due to patient choice, inability to attend appointments or patient death. After assessment by genetics services, family risk was formally categorised. The low risk category included 53 patients (47.7%), 43 patients (38.7%) were at moderate risk, and 15 patients (13.5%) were at high risk. The risk was also analysed according to the original referral required category, as shown in table 4. Four of the cases where family risk was considered high after genetic testing were originally deemed unnecessary for referral.
The incidence of CRC is increasing in young patients with seemingly few risk factors(9). Currently, the notion that younger age of onset is related to a poor prognosis is controversial; survival data is rather ambiguous and tumour characteristics are not consistently described for this subgroup of yCRC patients(2,8,9). Furthermore, no such study has been completed in Scotland. By establishing survival and characterising clinico-pathological features of this Scottish cohort, current knowledge and practice relating to yCRC may improve on both a local and international level.
In this retrospective study, survival in yCRC was found to be better than that in later onset CRC despite higher rates of advanced disease. Spanning an 11 year period up to December 2015, follow-up and survival data from 345 CRC patients aged under 55 years was collected. Despite a significant number of patients were diagnosed at late stage disease, the cohort had a greater overall five year survival of 63%, compared to 59% - the latest five year survival statistic for CRC across all age groups(15). This may reflect the fitness and relative lack of co-morbidities of these younger patients, making them better candidates for surgery, chemotherapy and radiotherapy. We found the five year survival of Dukes C and D patients to be 67% and 18%, respectively. Across all age groups, five year survival in patients with Dukes C disease is estimated at 63%, and Dukes D is 7%(15). This suggests, contrary to popular belief, that the young-onset cohort actually had similar or better outcomes than colorectal cancer patients overall. This superior stage-specific survival is also reflected in other multi-national studies(2,4,9,12). However, in contrast, these studies did not find survival outcomes to be greater even when unadjusted to stage. This is likely to be because these studies tended to focus on patient groups aged between 20 and 40 years, rather than aged under 55 years. When our results are adjusted to age-specific survival, those less than 40 years of age were also found to have a poorer prognosis. Interestingly, one study consistent with this result by Ballester et al. also found that - despite an overall better prognosis - yCRC patients had a higher incidence of recurrence and development of metastasis than later-onset disease. In this study, tumour recurrence occurred in 22.6% of patients. In a Korean study considering CRC across all ages, recurrent disease was found to occur in 18.3%(16). Although inconclusive, this is potentially an area for further investigation to detemine why yCRC patients may have a higher incidence of tumour recurrence.
This study also characterised the cohort in terms of clinicopathological features, which found locally advanced rectosigmoid disease to be typical of yCRC. Tumours were commonly located in the left side of the bowel, with a greater proportion of tumours (42.6%) occurring in the rectum – concurring with previous literature describing young-onset disease(7). Interestingly, a recent BMJ case-control study investigating oral antibiotic use and CRC risk found that antibiotic use decreased distal, or rectal, cancer risk, but increased proximal, or colon, cancer risk(17). Although our cohort is relatively small, it includes all the patients in a geographical area, including both urban & rural populations. We found the vast majority of yCRC patients to have a left-sided or rectal tumour, which would question whether increased antibiotic use is a significant risk factor for yCRC(17). Over 60% of patients presented with late stage (Dukes’ C or D) disease. According to cancer research statistics, in Scotland approximately 23% of CRC patients present with Dukes D disease and 25% with Dukes C disease(18). In this cohort, perhaps surprisingly, fewer patients presented with Dukes’ D disease (18%), although there were nearly 70% more patients (42%) diagnosed initially with Dukes’ C disease compared to the national incidence across all ages. Advanced disease prior to diagnosis may be explained by delays in patient presentation and diagnosis due to the relative rarity of the condition in comparison to the older population, as well as a lack of screening. In Scotland, screening does not begin until the age of 50. In contrast with previous studies investigating yCRC(12,19), incidence of poorly differentiated – or high-grade – histology was not found to be overrepresented in our patient group. An Australian study concurs with this finding, putting this discrepancy down to the subjective nature of determining tumour grade across the world(2).
Of those referred to genetic services, a significant proportion (18%) were found to be MSI positive. FH was often not documented sufficiently or, in some cases, not at all. FH is a major determining factor for referral to genetic services given the autosomal dominant inheritance pattern of Lynch syndrome. SIGN 2003 guidelines state that a ‘three generation family history should be taken from all patients with colorectal cancer’(14). This was not adhered to and hindered the assessment of whether a patient required genetic referral, both for the purposes of this audit and in clinical practice. The audit found that only 46% of patients categorised as requiring referral had indeed been referred to genetics as part of their cancer management. However, given the poor FH records, there were potentially more unidentified patients who required referral. This falls far below the audit standard; 54% of the unseen patients requiring referral potentially have an unidentified underlying genetic risk implicating not only their future health, but also their families. Failure to identify these high or moderate risk families may have grave repercussions on the mortality and morbidity of these patients, as having knowledge of this risk allows access to the appropriate screening and counselling. Interestingly, four of the 18 patients categorised as not requiring referral (therefore were aged over 50 years and had no known FH of CRC documented) who were seen and tested by genetics services were actually found to be carriers of Lynch syndrome, and as such fell into the high risk category. Perhaps insufficient FH documentation is responsible for these unexpected results. The findings of this study are in accordance with the published literature. A multi-centred English audit, similar to this, was conducted in 2011. Although using a different referral criteria in line with their own clinical practice, findings showed that the referral rate ranged from between only 35%-55%(10). The findings have also been echoed internationally; a Dutch study found that documentation of family history was sub-optimal, being correctly documented in only 16% of cases. 34% of patients with a complete FHrecorded were referred genetics services(20). In 2009, an Australian study showed even poorer outcomes with only 54% of patients having FH documented, and only 12% of patients being referred for formal genetic testing(21). Another 2012 study from Australia claimed only 38% of CRC patients were asked about their family by a health care provider(22). The reason behind these findings may have been due to documentation errors; perhaps if a negative FH was found on enquiry, no FH documentation was made at all. However, even if this were the case, opportunities for patient referral to genetic services are almost certain to have been missed.
Despite meticulous data collection from an 11 year time period with at least two years survival follow-up, this study does have its limitations. Firstly, the cohort size of 345 patients is relatively small. When considering the division of patients into further lesser subgroups (for example, by age), this has obtained results often with a poor statistical significance. The data collection process introduced a degree of bias since most patient information was collected from oncology notes and other hospital notes including ward clerking and pathology reports were referred to to a lesser degree as this was more time consuming. Where patient notes were deemed unavailable, it was likely that these patients did not receive any oncology treatment due to early stage disease requiring only surgical intervention. Two separate researchers collated the data. Despite efforts to ensure data collection was identical both times, there may have been minor discrepancies in methods, denotation and benchmarks. As the SIGN (2003) guidelines were in use during the first cohort, they were also used as the guideline standards for the second cohort, accepting that the SIGN guidelines were updated in December 2011, with a recommended change in clinical practice changed. These new guidelines aimed to further improve the uptake of genetic referrals, though differences between the 2003 and 2011 standards were negligible regarding young-onset CRC and did not affect this study(13)(14). Routine assessment for Lynch syndrome is now the standard assessment of a tumour, and patient referral is no longer required. Re-audit will allow ascertainment in change of policy. ARI’s pathology dataset was updated in July 2014 to include immunohistochemistry (IHC) analysis looking at microsatellite mismatch proficiency on all specimens from patients aged less than 50 years. Since then, in 2015, all patients having a resection for a diagnosis of colorectal cancer in the North East of Scotland have had the KRAS, BRAF and MSI status assessed on their surgical specimen, by either polymerase chain reaction (PCR), or more recently via a next-generation sequencing (NGS) panel. As patients with rectal cancer may require pre-operative therapy, IHC for microsatellite proficiency is carried out on biopsy specimens, as this may influence therapeutic options. Therefore, sigificant improvements have been made with regard to identification of hereditary cancer syndromes.
In this study, overall survival in the under 55s was found to surpass that of CRC across all ages. This is important to discuss with yCRC patients, as perceptions in the general population reckon that young adult patients with cancer have an inferior outcome compared to patients with later-onset disease. However, patients aged under 40 years were found to have more advanced disease than those aged over 40 and had a slightly poorer prognosis. Since the Scottish Bowel Cancer Screening Programme will not detect cases in patients under 50 years, healthcare professionals should be especially vigilant. A significant lack of appropriate patient referral to genetic services was found from 2005 to 2015. Thus, opportunities for identification of potential hereditary cancer syndromes and screening are likely to have been missed in a significant high-risk patient group. Although many patients were not tested for MSI, 18% of those who were tested were found to carry Lynch syndrome or FAP.
CRC = Colorectal cancer
yCRC = Young-onset colorectal cancer
MSI = Microsatellite instability
FAP = Familial adenomatous polyposis
SIGN = Scottish Intercollegiate Guidelines Network
ARI = Aberdeen Royal Infrimary
TNM = Tumour, Nodes, Metastasis
SD = Standard deviation
CI = Confidence interval
IBD = Inflammatory bowel disease
FH = Family history
IHC = Immunohistochemistry
PCR = Polymerase chain reaction
NGS = Next-generation sequencing
Ethics approval and consent to participate
No formal ethical approval was sought for this work, but the work follows the principles of the Declaration of Helsinki. This complies with the national guidelines(23). Patient consent for the study was deemed unnecessary given the retrospective nature; this is in accordance with the national regulations(24).
Consent for publication
Not applicable.
Availability of data and materials
All data generated during this study are included in this published article. The datasets used during the current study are available from the corresponding author on reasonable request.
Competing interests
There are no conflicts of interest to declare.
Funding
This work was supported by an institutional grant from the University of Aberdeen Summer Research Scholarship. The role of this grant was to financially support a medical student researcher from approximately July to August 2018. The funding body had no implications on the study design, collection, analysis or interpretation of data. The study was presented in poster format to the funding body in November 2018 at the University of Aberdeen Summer Research Symposium.
Authors' contributions
SP gathered data from 2010-2015, K Laurie gathered data from 2005-2009. SP accumulated and analaysed data. KL supervised throughout. LS provided datasets/designed study. ZM and AJ allowed access to INV. SP wrote the manuscript. All authors read and approved the final manuscript.
Acknowledgements
Not applicable
Table 1: Summarised SIGN 2003 guidelines defining moderate and high risk families
High Risk |
≥3 family members affected by CRC, or≥2 with CRC and one with endometrial. One affected relative ≤50, one must be first degree relative of another |
Medium Risk |
One first degree relative with CRC ≤45 |
|
Gene carriers (HNPCC) |
Two 1st relatives affected (one aged <55 years) |
|||
Untested 1st degree relatives of gene carriers |
≥2 relatives with CRC or endometrial cancer who are 1st degree relatives of each other, |
|
Table 2: Survival Data
|
Mean survival |
Five year survival (%) |
Overall |
96 |
63 |
Age group |
|
|
<30 |
47 |
57 |
30-39 |
62 |
57 |
40-49 |
88 |
68 |
50-54 |
96 |
62 |
Dukes' Staging |
|
|
A |
104 |
94 |
B |
102 |
82 |
C |
87 |
67 |
D |
32 |
18 |
Sex |
|
|
Male |
99 |
64 |
Female |
82 |
61 |
Presentation Type |
|
|
Elective |
90 |
69 |
Emergency |
61 |
39 |
Screening |
100 |
82 |
Table 3: Epidemiological and clinicopathological characteristics of patients by age
Characteristic |
Age (n/%) |
Total |
P value |
|||
<29 |
30-39 |
40-49 |
50-54 |
|||
Count |
6 (1.7%) |
34 (9.9%) |
132 (38.2%) |
173 (50.1%) |
345 |
|
Sex |
|
|
0.940 |
|||
Male |
3 (0.9%) |
19 (5.5%) |
69 (20%) |
91 (26%) |
182 |
|
Female |
3 (0.9%) |
15 (4.3%) |
63 (18%) |
82 (23.8%) |
163 |
|
Presentation |
|
|
<0.001 |
|||
Elective |
2 (0.6%) |
21 (6.1%) |
95 (27.5%) |
89 (25.8%) |
207 |
|
Emergency |
3 (0.9%) |
12 (3.5%) |
33 (9.6%) |
28 (8.1%) |
76 |
|
Screening |
0 |
0 |
1 (0.3%) |
52 (15.1%) |
53 |
|
Incidental |
0 |
0 |
1 (0.3%) |
1 (0.3%) |
2 |
|
Unknown |
1 (0.3%) |
1 (0.3%) |
2 (0.6%) |
3 (0.9%) |
7 |
|
Past Medical History |
|
|
|
|||
IBD |
0 |
4 (1.2%) |
3 (0.9%) |
7 (2.0%) |
14 |
0.243 |
Previous cancer |
0 |
1 (0.3%) |
4 (1.2%) |
9 (2.6%) |
14 |
0.728 |
Alcoholism |
0 |
2 (0.6%) |
12 (3.5%) |
14 (4.1%) |
28 |
0.949 |
Dukes’ Stage |
|
|
0.369 |
|||
A |
0 |
2 (0.6%) |
12 (3.5%) |
27 (7.8%) |
41 |
|
B |
2 (0.6%) |
5 (14.5%) |
37 (10.7%) |
46 (13.3%) |
90 |
|
C |
3 (0.9%) |
16 (4.6%) |
59 (17.1%) |
68 (19.7%) |
146 |
|
D |
1 (0.3%) |
10 (2.9%) |
23 (6.7%) |
31 (9.0%) |
65 |
|
Tumour Location |
|
|
0.895 |
|||
Right |
1 (0.3%) |
6 (1.7%) |
18 (5.2%) |
35 (10.1%) |
60 |
|
Left |
3 (0.9%) |
12 (3.5%) |
53 (15.4%) |
61 (17.7%) |
129 |
|
Rectal |
2 (0.6%) |
16 (4.6%) |
55 (15.9%) |
74 (21.4%) |
147 |
|
Transverse |
0 |
0 |
3 (0.9%) |
2 (0.6%) |
5 |
|
Tumour Differentiation |
|
|
0.482 |
|||
Well |
0 |
0 |
2 (0.6%) |
3 (0.9%) |
4 |
|
Moderate |
5 (1.4%) |
23 (6.7%) |
106 (30.7%) |
137 (39.7%) |
271 |
|
Poor |
1 (0.3%) |
8 (2.3%) |
17 (4.9%) |
16 (4.6%) |
42 |
|
Undocumented |
0 |
3 (0.9%) |
8 (2.3%) |
17 (4.9%) |
28 |
|
Management |
|
|
|
|||
Surgical |
5 (1.4%) |
27 (7.8%) |
117 (33.9%) |
155 (44.9%) |
304 |
0.486 |
Palliative |
1 (0.3%) |
6 (1.7%) |
14 (4.1%) |
16 (4.6%) |
37 |
0.486 |
Chemotherapy Received |
5 (1.4%) |
31 (9.0%) |
110 (31.9%) |
133 (38.6%) |
279 |
0.203 |
Radiotherapy Received |
0 |
14 (4.1%) |
42 (12.2%) |
61 (17.7%) |
117 |
0.236 |
Tumour Recurrence |
0 |
5 (1.4%) |
40 (11.6%) |
33 (9.6%) |
78 |
0.041 |
Patient Deceased |
3 (0.9%) |
14 (4.1%) |
42 (12.2%) |
55 (15.9%) |
114 |
0.537 |
Table 4: Referral category against risk
|
Categorised Risk (n) |
|
|||
Unable to |
Low |
Moderate |
High |
Total (n) |
|
Referral required |
28 |
42 |
12 |
11 |
93 |
Referral not required |
0 |
11 |
3 |
4 |
18 |
Total |
28 |
53 |
15 |
15 |
111 |
Actual family risk of CRC according to characterisation of requirement to genetic referral