This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Nano Express
Wantida Chaiyana
Chiang Mai University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This research is the first to investigate the anti-skin wrinkle properties of Cordyceps mili-taris extracts both in vitro and in vivo. Anti-skin wrinkle activities of C. militaris were investigated by means of matrix metalloproteinase-1 (MMP-1), elastase, and hyaluronidase inhibitions. Microemulsions and topical serum formulation containing C. militaris extract were developed. The anti-skin wrinkle efficacy and irritation properties of the topical serum formulations were clinically investigated in human volunteers. Cordycepin was identified as a major component of C. miliaris extract that was responsible for MMP-1, elastase, and hyaluronidase inhibition. The C. miliaris water extract possessed the most potent inhibition on MMP-1 (77.9 ± 5.3%) and elastase (84.4 ± 4.0%). Interestingly, CW was as a potent MMP-1 and elastase inhibitor as oleanolic acid and EGCG. CW was incorporated into the microemulsion with the smallest internal droplet size (146.1 ± 1.5 nm) and further developed as a topical serum formulation. The resulting serum formulation effectively enhanced skin moisture (42.2 ± 14.2%), increased the skin elasticity (39.9 ± 7.3%), and induced no skin irritation in 30 human volunteers. The effectiveness on the skin was detected after 1 week of the applications. Therefore, it was suggested as an effective anti-skin wrinkle formulation.
Keywords
Cordyceps militaris, microemulsion, cosmetic, anti-skin wrinkle, metalloproteinase-1, elastase, skin moisture, skin elasticity
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryInformation.docx
Fig. S1. Pseudoternary phase diagrams constructed using the mixture of Tween® 85 and butylene glycol (1:1) with various oil types, including apricot kernel oil (a), argan oil (b), avocado oil (c), canola oil (d), corn oil (e), jojoba oil (f), perilla oil (g), and sugar squalane (h). The dark area represents the microemulsion region. Fig. S2. Pseudoternary phase diagrams constructed using sugar squalane with the mixture of Tween® 85 and various types of co-surfactant, including glycerin (a), propylene glycol (b), and butylene glycol (c). The ratio of Tween® 85 to co-surfactant was 1:1. The dark area represents the microemulsion region. Fig. S3. Pseudoternary phase diagrams of microemulsion using sugar squalane with the mixture of Tween® 85 and propylene glycol in the weight ratio of 1:2 (a), 1:1 (b), 2:1 (c), 3:1 (d), 4:1 (e) and 5:1 (f). The dark area represents the area of microemulsion.
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 08 Jun, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Nano Express
Wantida Chaiyana
Chiang Mai University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 08 Jun, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This research is the first to investigate the anti-skin wrinkle properties of Cordyceps mili-taris extracts both in vitro and in vivo. Anti-skin wrinkle activities of C. militaris were investigated by means of matrix metalloproteinase-1 (MMP-1), elastase, and hyaluronidase inhibitions. Microemulsions and topical serum formulation containing C. militaris extract were developed. The anti-skin wrinkle efficacy and irritation properties of the topical serum formulations were clinically investigated in human volunteers. Cordycepin was identified as a major component of C. miliaris extract that was responsible for MMP-1, elastase, and hyaluronidase inhibition. The C. miliaris water extract possessed the most potent inhibition on MMP-1 (77.9 ± 5.3%) and elastase (84.4 ± 4.0%). Interestingly, CW was as a potent MMP-1 and elastase inhibitor as oleanolic acid and EGCG. CW was incorporated into the microemulsion with the smallest internal droplet size (146.1 ± 1.5 nm) and further developed as a topical serum formulation. The resulting serum formulation effectively enhanced skin moisture (42.2 ± 14.2%), increased the skin elasticity (39.9 ± 7.3%), and induced no skin irritation in 30 human volunteers. The effectiveness on the skin was detected after 1 week of the applications. Therefore, it was suggested as an effective anti-skin wrinkle formulation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryInformation.docx
Fig. S1. Pseudoternary phase diagrams constructed using the mixture of Tween® 85 and butylene glycol (1:1) with various oil types, including apricot kernel oil (a), argan oil (b), avocado oil (c), canola oil (d), corn oil (e), jojoba oil (f), perilla oil (g), and sugar squalane (h). The dark area represents the microemulsion region. Fig. S2. Pseudoternary phase diagrams constructed using sugar squalane with the mixture of Tween® 85 and various types of co-surfactant, including glycerin (a), propylene glycol (b), and butylene glycol (c). The ratio of Tween® 85 to co-surfactant was 1:1. The dark area represents the microemulsion region. Fig. S3. Pseudoternary phase diagrams of microemulsion using sugar squalane with the mixture of Tween® 85 and propylene glycol in the weight ratio of 1:2 (a), 1:1 (b), 2:1 (c), 3:1 (d), 4:1 (e) and 5:1 (f). The dark area represents the area of microemulsion.
Loading...