Malaria pathophysiology is not still fully understood. The main mechanisms of malaria involve the synergistic interactions between host and parasite. Although, the role of the spleen has been mentioned in various clinical forms of malaria, a supportive clinical evidence is still needed. We conducted a pilot study to determine the impact of the spleen functional state in different clinical forms of malaria. Ex vivo microsphiltration was used to assess the splenic function in patients received during routine consultation with mild malaria at the Kéniéroba health center, a high malaria endemic area in Mali. A total of 25 patients were enrolled for ex vivo microsphiltration. Spleen was non-palpable (Hackett stage 0) in two patients, palpable with deep inspiration (Hackett stage 1) in 22 patients and without deep inspiration (Hackett stage 2) in one patient, parasitaemia ranged from 5360 trophozoites/µl to 342720 trophozoites/µl with a mean parasitemia of 50774 trophozoites/µl ± 65540 trophozoites/µl. The mean hemoglobin level was 11.2g/dl ± 1.2 8.7-13.4. The retention rate of the infected red blood cell ranged from 11.11% to 94.44% with 65.4% ± 23.7% on average. A higher ex vivo retention rate of infected red blood cells was observed in patients with Hackett stage other than 0 (p= 0.03). This pilot study proved that it was feasible to use the ex vivo microsphiltration to explore the spleen filtering function in malaria patients.