This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research Article
Krishna Khairnar
CSIR-NEERI: National Environmental Engineering Research Institute CSIR
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9674-0817
License:
This work is licensed under a CC BY 4.0 License. Read Full License
It is becoming increasingly difficult in combating Multi-drug resistant (MDR) bacteria. MDR Staphylococcus aureus particularly methicillin-resistant S. aureus is one such notorious pathogen in clinical settings and the food industry. With increasing incidences of drug resistance and slow progress in developing new antibiotics, bacteriophages against pathogenic S. aureus are promising as antibacterial. We isolated Four local field MDR S. aureus from wastewater samples. We got a bacteriophage against an MDR S. aureus from a river-water sample. The bacteriophage was lytic and was stable at various temperatures ranging from − 20°C to 37°C. the bacteriophage was stable at a highly alkaline PH and had a narrow host range. Through genomic analysis, the bacteriophage DNA encodes 52 genes, and all predicted genes are on one strand, it also encodes a phage RNA polymerase; although it does not show similarity to any known staphylococcal bacteriophage, it shows similarity (91%) to Enterobacteriaceae phages. When surveying the research articles about Staphylococcal phages, we could find about the unclassified and Singleton-Staphylococcal phages.
Keywords
Methicillin-Resistant Staphylococcus aureus, Bacteriophage; Bacteriophage genome sequencing; Multi-Drug Resistance.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- Supplementary1highlightingthephenomenon16.04.2021.docx
Supplementary file 1: Highlighting the Phenomenon of Relative Difficulty in Isolating Bacteriophage against Staphylococcus aureus compared to Escherichia coli
- ARSupplementary2.docx
Supplementary file 2: Sampling details and host identification (It is a part of supplementary 1).
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 07 Jun, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Applied & Industrial Microbiology
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Krishna Khairnar
CSIR-NEERI: National Environmental Engineering Research Institute CSIR
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9674-0817
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 07 Jun, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Applied & Industrial Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
It is becoming increasingly difficult in combating Multi-drug resistant (MDR) bacteria. MDR Staphylococcus aureus particularly methicillin-resistant S. aureus is one such notorious pathogen in clinical settings and the food industry. With increasing incidences of drug resistance and slow progress in developing new antibiotics, bacteriophages against pathogenic S. aureus are promising as antibacterial. We isolated Four local field MDR S. aureus from wastewater samples. We got a bacteriophage against an MDR S. aureus from a river-water sample. The bacteriophage was lytic and was stable at various temperatures ranging from − 20°C to 37°C. the bacteriophage was stable at a highly alkaline PH and had a narrow host range. Through genomic analysis, the bacteriophage DNA encodes 52 genes, and all predicted genes are on one strand, it also encodes a phage RNA polymerase; although it does not show similarity to any known staphylococcal bacteriophage, it shows similarity (91%) to Enterobacteriaceae phages. When surveying the research articles about Staphylococcal phages, we could find about the unclassified and Singleton-Staphylococcal phages.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- Supplementary1highlightingthephenomenon16.04.2021.docx
Supplementary file 1: Highlighting the Phenomenon of Relative Difficulty in Isolating Bacteriophage against Staphylococcus aureus compared to Escherichia coli
- ARSupplementary2.docx
Supplementary file 2: Sampling details and host identification (It is a part of supplementary 1).
Loading...