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Research Article
Hai-Tao Pan
Shaoxing Maternity and Childcare Hospital: Shaoxing Women and Children's Hospital
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: Preeclampsia and gestational hypertension can cause impaired vascular function in offspring. In our previous work, we described the profiles of protein expression in umbilical artery tissues of patients with preeclampsia.
Methods: In order to gain insights into the mechanisms of vascular dysfunction in adult rats born by preeclampsia dams, we present analysis of thoracic aortic tissues by using iTRAQ isobaric tags and 2D nano LC-MS/MS.
Results: By using iTRAQ method, we analyzed 1825 proteins in which 106 proteins have shown significantly differential expression in thoracic aortic. Results from ingenuity pathway analysis (IPA) showed majority of proteins with differential expression (DEP)were associated with cardiovascular function. Further analysis indicated that glucose-6-phosphate dehydrogenase (G6PD), which is inhibited by miR-423-5p and activated by TP53, was most affect the cardiovascular function. The expression level of G6PD was up-regulated in thoracic aortic tissues as confirmed by western blot results. Two other vascular function proteins Cysteine and glycine-rich protein 2 (CSRP2) and Tubulin alpha-4A (TUBA4A) were upregulated as demonstrated by mass spectrometry (MS).
Conclusions: Although the results need further functional validation, these data provide novel findings relating to impaired vascular function in adult offsprings of preeclamptic mothers.
Keywords
Preeclampsia, Offspring, iTRAQ, Gamete and Embryo-Fetal Origins of Adult Diseases, Thoracic aortic
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementalFigure1.tif
Supplemental Figure 1. DEPs were strongly related to 24 subcategories of "Disease and Disorder".
- SupplementalFigure2.tif
Supplemental Figure 2. DEPs were strongly related to 19 subcategories of “Physiological System Development and Functions”.
- SupplementalTable1.xlsx
Supplemental Table 1. 1825 non-redundant proteins in thoracic aortic with high confidence.
- SupplementalTable2.xlsx
Supplemental Table 2. 106 proteins showed differential expression between preeclampsia group with respect to the control group, 75 proteins were elevated whereas 31 were decreased.
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CURRENT STATUS: Published
View the published article at Clinical Proteomics.
Version 1
Posted 03 Jun, 2021
No community comments so far
Editorial decision: Major revision
On 26 Jun, 2021
Review #1 received
Received 06 Jun, 2021
Review #2 received
Received 06 Jun, 2021
Reviews received
Received 02 Jun, 2021
Reviewers invited
Invitations sent on 02 Jun, 2021
Reviewer #2 agreed
On 01 Jun, 2021
Reviewer #1 agreed
On 01 Jun, 2021
Editor assigned
On 27 May, 2021
Submission checks complete
On 26 May, 2021
Editor invited
On 26 May, 2021
First submitted
On 25 May, 2021
Metrics
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PDF Downloads: ...
HTML Views: ...
Subject Areas
Molecular Biology
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Clinical Proteomics.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Hai-Tao Pan
Shaoxing Maternity and Childcare Hospital: Shaoxing Women and Children's Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Clinical Proteomics.
Version 1
Posted 03 Jun, 2021
No community comments so far
Editorial decision: Major revision
On 26 Jun, 2021
Review #1 received
Received 06 Jun, 2021
Review #2 received
Received 06 Jun, 2021
Reviews received
Received 02 Jun, 2021
Reviewers invited
Invitations sent on 02 Jun, 2021
Reviewer #2 agreed
On 01 Jun, 2021
Reviewer #1 agreed
On 01 Jun, 2021
Editor assigned
On 27 May, 2021
Submission checks complete
On 26 May, 2021
Editor invited
On 26 May, 2021
First submitted
On 25 May, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Molecular Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Clinical Proteomics.
Background: Preeclampsia and gestational hypertension can cause impaired vascular function in offspring. In our previous work, we described the profiles of protein expression in umbilical artery tissues of patients with preeclampsia.
Methods: In order to gain insights into the mechanisms of vascular dysfunction in adult rats born by preeclampsia dams, we present analysis of thoracic aortic tissues by using iTRAQ isobaric tags and 2D nano LC-MS/MS.
Results: By using iTRAQ method, we analyzed 1825 proteins in which 106 proteins have shown significantly differential expression in thoracic aortic. Results from ingenuity pathway analysis (IPA) showed majority of proteins with differential expression (DEP)were associated with cardiovascular function. Further analysis indicated that glucose-6-phosphate dehydrogenase (G6PD), which is inhibited by miR-423-5p and activated by TP53, was most affect the cardiovascular function. The expression level of G6PD was up-regulated in thoracic aortic tissues as confirmed by western blot results. Two other vascular function proteins Cysteine and glycine-rich protein 2 (CSRP2) and Tubulin alpha-4A (TUBA4A) were upregulated as demonstrated by mass spectrometry (MS).
Conclusions: Although the results need further functional validation, these data provide novel findings relating to impaired vascular function in adult offsprings of preeclamptic mothers.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementalFigure1.tif
Supplemental Figure 1. DEPs were strongly related to 24 subcategories of "Disease and Disorder".
- SupplementalFigure2.tif
Supplemental Figure 2. DEPs were strongly related to 19 subcategories of “Physiological System Development and Functions”.
- SupplementalTable1.xlsx
Supplemental Table 1. 1825 non-redundant proteins in thoracic aortic with high confidence.
- SupplementalTable2.xlsx
Supplemental Table 2. 106 proteins showed differential expression between preeclampsia group with respect to the control group, 75 proteins were elevated whereas 31 were decreased.
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