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Research Article
Chen Chen
Peking University Ditan Teaching Hospital
Corresponding Author
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Enterococcus faecalis, a major nosocomial pathogen, has become a top leading cause of hospital-acquired infections including urinary tract infections, endocarditis and bacteremia. It is important to study the epidemiology and virulence characteristics of E. faecalis isolates in order to tailor infection prevention and antibiotic prescribing. In this study, comparative genomic analysis was conducted on 537 isolates from different human origins. The isolates from bloodstream and intra-abdominal lining had the largest and smallest average genome size respectively, while the isolates from open natural orifices (gastrointestinal tract, urinary tract, respiratory tract, wound and eye) had medium average genome size. The phylogenetic relationships were expounded that the strain isolation niche is uncorrelated with strain phylogeny. Six clonal complexes generally appeared in different isolation sources. Furthermore, genomic analysis revealed differences at the accessory genome, the functions of different genes mainly pointed to the virulence, drug resistance and metabolism of E. faecalis. Interestingly, fsr quorum sensing system genes affecting biofilm formation had a highest proportion in the blood-derived strains. This study showed the genomic characteristics of different human origins and suggested that fsr quorum-sensing system maybe a contributing factor of bacteremia due to E. faecalis infection.
Keywords
Enterococcus faecalis, Genomes, fsr quorum sensing system, Antibiotic resistance genes
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No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.xlsx
Table S1: the background information of all 537 E. faecalis isolates;
- TableS2.xlsx
Table S2: the p-values of 2546 genes of all 8 isolation groups;
- TableS3.xlsx
Table S3: the coverage and identity of antibiotic resistance genes in 537 E. faecalis isolates;
- TableS4.xlsx
Table S4: the coverage and identity of virulence genes in 537 E. faecalis isolates;
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Chen Chen
Peking University Ditan Teaching Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 09 Jun, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Infectious Diseases
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Enterococcus faecalis, a major nosocomial pathogen, has become a top leading cause of hospital-acquired infections including urinary tract infections, endocarditis and bacteremia. It is important to study the epidemiology and virulence characteristics of E. faecalis isolates in order to tailor infection prevention and antibiotic prescribing. In this study, comparative genomic analysis was conducted on 537 isolates from different human origins. The isolates from bloodstream and intra-abdominal lining had the largest and smallest average genome size respectively, while the isolates from open natural orifices (gastrointestinal tract, urinary tract, respiratory tract, wound and eye) had medium average genome size. The phylogenetic relationships were expounded that the strain isolation niche is uncorrelated with strain phylogeny. Six clonal complexes generally appeared in different isolation sources. Furthermore, genomic analysis revealed differences at the accessory genome, the functions of different genes mainly pointed to the virulence, drug resistance and metabolism of E. faecalis. Interestingly, fsr quorum sensing system genes affecting biofilm formation had a highest proportion in the blood-derived strains. This study showed the genomic characteristics of different human origins and suggested that fsr quorum-sensing system maybe a contributing factor of bacteremia due to E. faecalis infection.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.xlsx
Table S1: the background information of all 537 E. faecalis isolates;
- TableS2.xlsx
Table S2: the p-values of 2546 genes of all 8 isolation groups;
- TableS3.xlsx
Table S3: the coverage and identity of antibiotic resistance genes in 537 E. faecalis isolates;
- TableS4.xlsx
Table S4: the coverage and identity of virulence genes in 537 E. faecalis isolates;
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