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Research Article
L. Stronati
Sapienza University of Rome
Corresponding Author
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Microalgae are currently considered as alternative sustainable resources for high-value bioproducts such as omega3 polyunsaturated fatty acids (PUFAs). Probiotics are assumed to benefit human health by their direct actions on the composition and function of gut microbiota. Aims of the study are: 1) to set up the anaerobic growth of the probiotic Lactobacillus reuteri (L. reuteri) in the omega3-rich microalga Isochrysis galbana (I. galbana); 2) to assess the potential role of the obtained fermented compound (FC) to control the harmfulness of adherent invasive Escherichia coli (AIEC) to intestinal epithelial cells.
I. galbana powder solubilized in PBS was used for the anaerobic growth of L. reuteri. The lipidic content of I. galbana and FC was analyzed by GC-MS. Colorectal adenocarcinoma cells CACO2 and the AIEC strain LF82 were used for in vitro experiments.
I. galbana is shown to be an excellent culture medium for growing L. reuteri. The obtained FC significantly reduces the AIEC adhesiveness and invasiveness to intestinal epithelial cells.
We show for the first time that microalgae may represent an innovative culture medium to grow probiotics in anaerobiosis. The obtained FC shows beneficial properties for human health by controlling the harmfulness of AIEC bacteria.
Keywords
galbana, reuteri, aiec, obtained, cells, microalgae, omega3, probiotics
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No competing interests reported.
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Posted 04 Jun, 2021
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On 26 Jul, 2021
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On 30 Jun, 2021
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A new preprint design is coming!
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
L. Stronati
Sapienza University of Rome
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 04 Jun, 2021
No community comments so far
Editorial decision: Major revision
On 26 Jul, 2021
Reviews received
Received 01 Jul, 2021
Reviewers agreed
On 30 Jun, 2021
Reviewers invited
Invitations sent on 27 Jun, 2021
Editor assigned
On 27 Jun, 2021
Editor invited
On 01 Jun, 2021
Submission checks complete
On 31 May, 2021
First submitted
On 26 May, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Microalgae are currently considered as alternative sustainable resources for high-value bioproducts such as omega3 polyunsaturated fatty acids (PUFAs). Probiotics are assumed to benefit human health by their direct actions on the composition and function of gut microbiota. Aims of the study are: 1) to set up the anaerobic growth of the probiotic Lactobacillus reuteri (L. reuteri) in the omega3-rich microalga Isochrysis galbana (I. galbana); 2) to assess the potential role of the obtained fermented compound (FC) to control the harmfulness of adherent invasive Escherichia coli (AIEC) to intestinal epithelial cells.
I. galbana powder solubilized in PBS was used for the anaerobic growth of L. reuteri. The lipidic content of I. galbana and FC was analyzed by GC-MS. Colorectal adenocarcinoma cells CACO2 and the AIEC strain LF82 were used for in vitro experiments.
I. galbana is shown to be an excellent culture medium for growing L. reuteri. The obtained FC significantly reduces the AIEC adhesiveness and invasiveness to intestinal epithelial cells.
We show for the first time that microalgae may represent an innovative culture medium to grow probiotics in anaerobiosis. The obtained FC shows beneficial properties for human health by controlling the harmfulness of AIEC bacteria.
Figure 1
Figure 2
Figure 3
Figure 4
No competing interests reported.
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