High Myopia and Macular Vascular Density: An Optical Coherence Tomography Angiography Study

DOI: https://doi.org/10.21203/rs.3.rs-564947/v1

Abstract

Objective: To investigate whether there are differences in macular vascular density (VD) between patients with high-myopia (HM) and those with non-high myopia (NHM) using Optical Coherence Tomography Angiography (OCTA).

Method: OCTA was performed on 35 eyes with HM with spherical equivalence (SE) >-6.00D and 35 eyes with NHM with SE £-6.00D. Vascular densities of the macula (overall macula, fovea, parafovea, superior hemi and inferior hemi) were measured in each of the superficial, deep and choriocapillaris layers of the retina.

Results: In the superficial retinal layer, overall macular VFD was significantly higher in the NHM compared to the HM group (51.27±3.74 vs. 48.07±5.69, p<0.05). There were significant differences between the NHM and HM in parafovea (52.58±5.78 vs. 49.4 ±6.43, p<0.05), superior-hemi (53.38±4.03 vs 49.78±6.84, p<0.05) and inferior-hemi regions (53.49±4.61 vs 49.05±6.41, p<0.05), but not in the fovea region. Similarly, in the deep retinal layer, overall macular VFD was significantly higher in the NHM group compared to the HM group (58.69±2.46 vs. 56.90±4.08, p<0.05). There was significant differences between the HM and NHM in superior-hemi region (61.97±2.68 vs. 60.08±3.98, p<0.05), but not in the fovea, parafovea, and inferior-hemi region. In the choriocapillaris, there was no difference in the overall macular VFD, nor any of the individual sectors between the HM and the NHM groups.

Conclusion: VFD in the superficial and deep retinal layers of the macula are significantly increased in the NHM compared to HM eyes. This is not the case in the choroidal capillary layers of the retina. 

Background

Uncorrected refractive error is common, affecting approximately 108 million people worldwide. It is also the second leading cause of blindness worldwide [1]. In a Japanese population study, 12.2% of vision impairment was caused by myopic macular degeneration)[2]. Among other population-based studies, the prevalence of high myopia (HM) has been estimated to comprise 5–10% of diagnosed myopias, and 1–4% of the general population [3, 4]. In the US population, it is estimated that HM has increased 8-fold over 30 years, from 0.2–1.6% [5]. Myopia therefore poses a significant social economic burden on both developing and developed countries.

High myopia is significantly associated with morphological changes within the retina [68]. It increases the risk of pathologic myopia leading to retinal atrophy, lattice degeneration, lacquer cracks, choroidal neovascularization, and retinal detachment [9]. In Japan, myopic maculopathy was reported to be the primary cause of legal blindness and the third most prevalent cause of poor vision [10]. In Western populations, myopic maculopathy is also a significant cause of legal blindness [11].

OCTA is a technique that measure light reflectance of moving red cells to visualize the retinal vasculature and microcirculation. It is quick, non-invasive and provides quantitative, blood flow and structural information [12]. We attempted to utilize OCTA to investigate differences in macular vascular density in HM compared to non-high myopia (NHM) patients, with the aim of contributing to the etiological understanding of pathologic myopia.

Methods

Participants

This is a prospective, cross-sectional study. Seventy eyes with myopia were recruited from December 2014 to December 2020 in the Department of Ophthalmology in Peking Union Medical College Hospital, Chinese Academy of Medical Sciences. Thirty-five eyes with HM and 35 eyes with NHM were recruited. HM was defined as a spherical equivalence (SE) >-6.00D, and NHM defined as a SE ≤-6.00D. Only the OCTA index of the right eyes of the participants were recorded. Exclusion criteria included any history of other any ocular diseases, systemic diseases that may affect the ocular circulation and previous intraocular surgery or ocular injury.

Optical Coherence Tomography Angiography

OCTA scans were obtained using OCT (Optovue, Fremont, California, USA) Angio-Retina mode (3x3 mm). Split-spectrum amplitude decorrelation angiography (SSADA) was utilized as the signal process algorithm. This has been described in detail in previous literature. Vascular density (VD) is the percentage of the sample area occupied by vessel lumens. OCTA images excluded from this study include those with poor signal strength (< 40), images with severe artefacts, images which demonstrates epiretinal membrane, foveoschisis, macular holes, choroidal neovascularization and retinal detachments. A 3x3mm scanning area of the macula was acquired and divided into the superficial, deep and choroidal layers. Each of the layers were automatically segmented by the software. Results were analyzed with the Optovue software. VD of the overall macula was calculated, and each region was calculated separately (fovea, parafovea, superior-hemi and inferior-hemi).

Statistical analysis

SPSS software package (SPSS 12.0) was utilized to perform statistical analysis. The mean and standard deviation of the main parameters were calculated. The VD were compared between the two groups. A p-value calculated using the T-test, and a level of < 0.05 was considered statistically significant.

Table 1

Corneal Conditions of NHM and HM patients

 

NHM

HM

T

p-value

K1

43.45 ± 16.8

44.07 ± 14.2

1.719

0.090

K2

42.53 ± 14.9

42.71 ± 13.0

0.509

0.614

Avek

42.91 ± 15.0

43.44 ± 12.9

1.609

0.117

CCT

536.94 ± 33.96

543.82 ± 33.32

0.750

0.458

Avek: average k; CCT: central corneal thickness

Results

Demographics

Seventy right eyes (32 men and 38 women) were included in this study. The NHM group (n = 35) contained 18 women and 17 men with a mean age of 27.3 ± 4.5 years. The HM group (n = 35) contained 20 women and 15 men with a mean age of 25.6 ± 5.4 years. There were no significant differences in gender, age, K1, K2, Avek and CCT between the two groups (Table 1).

Macular density

The superficial retinal layer showed an overall decrease in VFD in the HM group (Table 2). The overall macular VD were 51.27 ± 3.74 and 48.07 ± 5.69 in the NHM and HM groups, respectively (p < 0.05). The multiple sectorial comparisons showed that there were significant reductions in the VFD in the HM group in the parafovea (52.58 ± 5.78 vs 49.40 ± 6.43, p < 0.05), superior-hemi (53.38 ± 4.03 vs 49.78 ± 6.84, p < 0.05) and inferior-hemi regions (53.49 ± 4.61 vs 49.05 ± 6.41, p < 0.05), but not in the fovea region (28.75 ± 5.22 vs 28.56 ± 6.55, p > 0.05).

Table 2

Macular Vascular Density in Superficial Retinal Layer of NHM and HM patients

Superficial Retinal Layer of Macula

NHM

HM

T

p-value

Overall

51.27 ± 3.74

48.07 ± 5.69

2.503

0.017

Fovea

28.75 ± 5.22

28.56 ± 6.55

0.139

0.890

Parafovea

52.58 ± 5.78

49.40 ± 6.43

2.100

0.043

Superior-Hemi Region

53.38 ± 4.03

49.78 ± 6.84

2.471

0.019

Inferior-Hemi Region

53.49 ± 4.61

49.05 ± 6.41

2.990

0.005

The deep retinal layer showed also an overall decrease in macular VFD in the HM group (Table 3). The overall macular VFD were 58.69 ± 2.46 and 56.90 ± 4.08 in the NHM and the HM groups, respectively (p < 0.05). In the individual sectorial comparisons, only the superior-hemi region showed a reduction in VFD in the HM group (61.97 ± 2.68 vs 60.08 ± 3.98, p < 0.05). There were no significant differences in the in the fovea (25.24 ± 6.04 vs 24.98 ± 6.97), parafovea (62.44 ± 2.30 vs 61.10 ± 3.55) and inferior-hemi region (62.90 ± 2.43 vs 62.11 ± 3.50) (p > 0.05) (Table 3).

Table 3

Macular Vascular Density in the Deep Retinal Layer of NHM and HM patients

Deep Retinal Layer of Macula

NHM

HM

T

p-value

Overall

58.69 ± 2.46

56.90 ± 4.08

2.127

0.041

Fovea

25.24 ± 6.04

24.98 ± 6.97

0.163

0.872

Parafovea

62.44 ± 2.30

61.10 ± 3.55

1.845

0.074

Superior-Hemi Region

61.97 ± 2.68

60.08 ± 3.98

2.285

0.029

Inferior-Hemi Region

62.90 ± 2.43

62.11 ± 3.50

1.122

0.270

The choriocapillaris showed no significant differences in the HM and NHM groups overall and in each of the individual sectors (Table 4). The overall macular VFD were 65.50 ± 1.62 and 64.69 ± 2.29 in the HM and NHM groups respectively (p > 0.05). There was no significant differences between the HM and NHM patients in the fovea (65.11 ± 3.12 vs 63.48 ± 5.48), parafovea (65.39 ± 1.65 vs 64.56 ± 2.22), superior-hemi region(65.31 ± 1.65 vs 64.24 ± 3.15) and inferior-hemi region (65.49 ± 1.76 vs 64.86 ± 2.57) (p > 0.05).

Table 4

Macular Vascular Density in the Choriocapillaris of NHM and HM Patients

Choriocapillaris

NHM

HM

T

p-value

Overall

65.50 ± 1.62

64.69 ± 2.29

1.522

0.137

Fovea

65.11 ± 3.12

63.48 ± 5.48

1.518

0.138

Parafovea

65.39 ± 1.65

64.56 ± 2.22

1.598

0.119

Superior-Hemi Region

65.31 ± 1.65

64.24 ± 3.15

1.653

0.108

Inferior-Hemi Region

65.49 ± 1.76

64.86 ± 2.57

1.088

0.284

Discussion

High myopia is well-known to be associated with significant histopathological changes of the retina [8] and can lead to myopic maculopathy. Various degenerative changes can become evident in the posterior segment. In many developed countries, myopic maculopathy remains one of the key causes of visual impairment [13].

Although the etiology of myopic maculopathy is still elusive, previous literature has suggested that increased radius of the eyeball in HM eyes lead to reduced blood circulation [1416]. There is evidence that reduced choroidal blood flow over a prolonged period of time leads to release of vascular endothelial growth factor (VEGF) and consequent myopic choroidal neovascularization (CNV) [17]. Myopic CNV was shown to have a prevalence rate of 10–11% over the period of 12 years in one study [18]. The pathogenesis of myopic maculopathy may stem from a similar pathophysiological pathway.

Previous studies of retinal vasculature in HM eyes have focused on the use of time domain OCTs (TD-OCT) and Colour Doppler Imaging (CDIs). Axial length has been correlated with regional variations of retinal thickness [19] and negatively correlated with choroidal or retinal blood flow [14, 6]. Such studies have had difficulty visualizing the microvasculature, and experienced poor differentiation of static tissue to vasculature. Other techniques which have being utilized to investigate retinal blood flow include fluorescein angiography (FA). The utility of FA in HM is low due to its invasive and qualitative nature. Any potential side effects are further avoided in OCTA.

OCTA allows detailed, three-dimensional study of vasculature down to the capillary level. It utilizes the intrinsic motion of blood cells present in the vascular networks, offering a non-invasive and rapid test without the need for intravenous contrast [20].

In our study, OCTA was utilized to compare the macular VD of patients with NHM and HM. The SE of -6.00D was utilized to differentiate between the two groups. Our study demonstrates that HM is associated with a reduced macular VD. This is supported by the literature. Multiple studies have demonstrated increasing macular VD being associated with increasing myopia [21, 22]. This is not consistent within the literature, as some studies have demonstrated only differences in certain regions and layers [23] whilst others studies have not demonstrated differences between high myopic and non-high myopic patients [24, 25]. The difference in the literature could be attributable to selection of population. Studies which demonstrated non-significant difference between VD of myopic and high-myopic patients had relatively narrow age ranges of patients [24, 25]. Although the pathogenesis of myopic maculopathy is still relatively unknown, prognosis of patients with myopic maculopathy worsens with age [26]. With increasing age, a greater reduction of macular VD occurs. In sample sizes with younger patients, changes in VD may not be reflected.

In conclusion, our study has demonstrated negative correlations between high myopia and both the superficial and deep retinal vascular flow density readings as attained by OCTA.

Declarations

Ethical Approval and Consent to Participate:

All patients gave their informed consent for their anonymized data to be submitted for audit and publication. The institutional ethics committee of Chinese Academy of Medical Sciences, Peking Union Medical College Hospital approved this study. All methods were carried in line with ethics approval.

Consent to Publish:

Not applicable

Availability of data and materials:

Data supporting the results reported in the article are not public but can be accessed after communicating with the corresponding author. 

Competing Interests:

The authors declare that they have no competing interest.

Funding:

This work was supported by The Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences (2018PT32029).

Author Contributions:

All authors had input into the conception of the study. YJ undertook data extraction. YJ and TCL wrote and revised the main manuscript text. All authors reviewed the manuscript and approved it for publication. 

Acknowledgements:

Not applicable

References

  1. He M, Huang W, Zheng Y, Huang L, Ellwein LB (2007) Refractive error and visual impairment in school children in rural southern China. Ophthalmology 114 (2):374-382. doi:10.1016/j.ophtha.2006.08.020
  2. Yamada M, Hiratsuka Y, Roberts CB, Pezzullo ML, Yates K, Takano S, Miyake K, Taylor HR (2010) Prevalence of visual impairment in the adult Japanese population by cause and severity and future projections. Ophthalmic Epidemiol 17 (1):50-57. doi:10.3109/09286580903450346
  3. Wong TY, Ferreira A, Hughes R, Carter G, Mitchell P (2014) Epidemiology and disease burden of pathologic myopia and myopic choroidal neovascularization: an evidence-based systematic review. Am J Ophthalmol 157 (1):9-25 e12. doi:10.1016/j.ajo.2013.08.010
  4. Vongphanit J, Mitchell P, Wang JJ (2002) Prevalence and progression of myopic retinopathy in an older population. Ophthalmology 109 (4):704-711
  5. Vitale S, Sperduto RD, Ferris FL, 3rd (2009) Increased prevalence of myopia in the United States between 1971-1972 and 1999-2004. Arch Ophthalmol 127 (12):1632-1639. doi:10.1001/archophthalmol.2009.303
  6. Avetisov ES, Savitskaya NF (1977) Some features of ocular microcirculation in myopia. Ann Ophthalmol 9 (10):1261-1264
  7. Xu L, Li Y, Wang S, Wang Y, Wang Y, Jonas JB (2007) Characteristics of highly myopic eyes: the Beijing Eye Study. Ophthalmology 114 (1):121-126. doi:10.1016/j.ophtha.2006.05.071
  8. Jonas JB, Xu L (2014) Histological changes of high axial myopia. Eye (Lond) 28 (2):113-117. doi:10.1038/eye.2013.223
  9. Morgan IG, Ohno-Matsui K, Saw SM (2012) Myopia. Lancet 379 (9827):1739-1748. doi:10.1016/s0140-6736(12)60272-4
  10. Liu HH, Xu L, Wang YX, Wang S, You QS, Jonas JB (2010) Prevalence and progression of myopic retinopathy in Chinese adults: the Beijing Eye Study. Ophthalmology 117 (9):1763-1768. doi:10.1016/j.ophtha.2010.01.020
  11. Buch H, Vinding T, La Cour M, Appleyard M, Jensen GB, Nielsen NV (2004) Prevalence and causes of visual impairment and blindness among 9980 Scandinavian adults: the Copenhagen City Eye Study. Ophthalmology 111 (1):53-61
  12. de Carlo TE, Romano A, Waheed NK, Duker JS (2015) A review of optical coherence tomography angiography (OCTA). International Journal of Retina and Vitreous 1:5. doi:10.1186/s40942-015-0005-8
  13. Resnikoff S, Pascolini D, Mariotti SP, Pokharel GP (2008) Global magnitude of visual impairment caused by uncorrected refractive errors in 2004. Bull World Health Organ 86 (1):63-70
  14. Akyol N, Kukner AS, Ozdemir T, Esmerligil S (1996) Choroidal and retinal blood flow changes in degenerative myopia. Can J Ophthalmol 31 (3):113-119
  15. Moriyama M, Ohno-Matsui K, Futagami S, Yoshida T, Hayashi K, Shimada N, Kojima A, Tokoro T, Mochizuki M (2007) Morphology and long-term changes of choroidal vascular structure in highly myopic eyes with and without posterior staphyloma. Ophthalmology 114 (9):1755-1762. doi:10.1016/j.ophtha.2006.11.034
  16. Wakabayashi T, Ikuno Y (2010) Choroidal filling delay in choroidal neovascularisation due to pathological myopia. Br J Ophthalmol 94 (5):611-615. doi:10.1136/bjo.2009.163535
  17. Silva R (2012) Myopic maculopathy: a review. Ophthalmologica 228 (4):197-213. doi:10.1159/000339893
  18. Hayashi K, Ohno-Matsui K, Shimada N, Moriyama M, Kojima A, Hayashi W, Yasuzumi K, Nagaoka N, Saka N, Yoshida T, Tokoro T, Mochizuki M (2010) Long-term pattern of progression of myopic maculopathy: a natural history study. Ophthalmology 117 (8):1595-1611, 1611 e1591-1594. doi:10.1016/j.ophtha.2009.11.003
  19. Lam DS, Leung KS, Mohamed S, Chan WM, Palanivelu MS, Cheung CY, Li EY, Lai RY, Leung CK (2007) Regional variations in the relationship between macular thickness measurements and myopia. Invest Ophthalmol Vis Sci 48 (1):376-382. doi:10.1167/iovs.06-0426
  20. Wang RK (2010) Optical Microangiography: A Label Free 3D Imaging Technology to Visualize and Quantify Blood Circulations within Tissue Beds in vivo. IEEE J Sel Top Quantum Electron 16 (3):545-554. doi:10.1109/jstqe.2009.2033609
  21. Fan H, Chen HY, Ma HJ, Chang Z, Yin HQ, Ng DS, Cheung CY, Hu S, Xiang X, Tang SB, Li SN (2017) Reduced Macular Vascular Density in Myopic Eyes. Chin Med J (Engl) 130 (4):445-451. doi:10.4103/0366-6999.199844
  22. Li M, Yang Y, Jiang H, Gregori G, Roisman L, Zheng F, Ke B, Qu D, Wang J (2017) Retinal Microvascular Network and Microcirculation Assessments in High Myopia. Am J Ophthalmol 174:56-67. doi:10.1016/j.ajo.2016.10.018
  23. Hassan M, Sadiq MA, Halim MS, Afridi R, Soliman MK, Sarwar S, Agarwal A, Do DV, Nguyen QD, Sepah YJ (2017) Evaluation of macular and peripapillary vessel flow density in eyes with no known pathology using optical coherence tomography angiography. Int J Retina Vitreous 3:27. doi:10.1186/s40942-017-0080-0
  24. Mo J, Duan A, Chan S, Wang X, Wei W (2017) Vascular flow density in pathological myopia: an optical coherence tomography angiography study. BMJ Open 7 (2):e013571. doi:10.1136/bmjopen-2016-013571
  25. Yang S, Zhou M, Lu B, Zhang P, Zhao J, Kang M, Wang R, Wang F, Sun X (2017) Quantification of Macular Vascular Density Using Optical Coherence Tomography Angiography and Its Relationship with Retinal Thickness in Myopic Eyes of Young Adults. J Ophthalmol 2017:1397179. doi:10.1155/2017/1397179
  26. Shih YF, Ho TC, Hsiao CK, Lin LLK (2006) Visual outcomes for high myopic patients with or without myopic maculopathy: a 10 year follow up study. The British Journal of Ophthalmology 90 (5):546-550. doi:10.1136/bjo.2005.081992