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Background
Huperzia serrata (H. serrata) produces various types of effectively lycopodium alkaloids, especially Huperzine A (HupA), which is a promising drug for the treatment of Alzheimer’s disease. Numerous studies focused on the chemistry, bioactivities, toxicology, and clinical trials of HupA, however, the public genomic and transcriptomic resources are very limited for H. serrata research, especially for the selection of optimum reference genes.
Results
Based on the full-length transcriptome datasets and previous studies, thirteen candidate reference genes were selected in different tissue of H. serrata. Then, two optimal reference genes GAPDHB and HisH2A were confirmed by various analysis softwares. In order to further verify the accuracy of the two reference genes, they were used to analyze the expression patterns of four HupA-biosynthetic related genes (lysine decarboxylas, RS-norcoclaurine 6-O-methyltransferase, cytochrome P45072A1 and copper amine oxidase). The data suggested that the expression trends of HupA-biosynthetic related genes were consistent with them in transcriptome sequencing in different tissue of H. serrata.
Conclusions
This study screened the best reference genes GAPDHB and HisH2A in different tissues of H. serrata, which provides suitable normalization for analyzing the expression of HupA-biosynthetic gene in transcriptional level in H. serrata.
Keywords
Huperzia serrata, Huperzine A, RT-qPCR, Reference Gene
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 08 Jun, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Huperzia serrata (H. serrata) produces various types of effectively lycopodium alkaloids, especially Huperzine A (HupA), which is a promising drug for the treatment of Alzheimer’s disease. Numerous studies focused on the chemistry, bioactivities, toxicology, and clinical trials of HupA, however, the public genomic and transcriptomic resources are very limited for H. serrata research, especially for the selection of optimum reference genes.
Results
Based on the full-length transcriptome datasets and previous studies, thirteen candidate reference genes were selected in different tissue of H. serrata. Then, two optimal reference genes GAPDHB and HisH2A were confirmed by various analysis softwares. In order to further verify the accuracy of the two reference genes, they were used to analyze the expression patterns of four HupA-biosynthetic related genes (lysine decarboxylas, RS-norcoclaurine 6-O-methyltransferase, cytochrome P45072A1 and copper amine oxidase). The data suggested that the expression trends of HupA-biosynthetic related genes were consistent with them in transcriptome sequencing in different tissue of H. serrata.
Conclusions
This study screened the best reference genes GAPDHB and HisH2A in different tissues of H. serrata, which provides suitable normalization for analyzing the expression of HupA-biosynthetic gene in transcriptional level in H. serrata.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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