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Chromosome segregation in B. subtilis is highly heterogeneous
Peter Graumann
Philipps-Universitat Marburg
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8033-5171
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Research Notes.
Objective: The bacterial cell cycle comprises initiation of replication and ensuing elongation, concomitant chromosome segregation (in some organisms with a delay termed cohesion), and finally cell division. By quantifying the number of origin and terminus regions in exponentially growing Bacillus subtilis cells, and after induction of DNA damage, we aimed at determining cell cycle parameters at different growth rates at a single cell level.
Results: B. subtilis cells are mostly mero-oligoploid during fast growth and diploid during slow growth. However, we found that the number of replication origins and of termini is highly heterogeneous within the cell population at two different growth rates, and that even at slow growth, a majority of cells attempts to maintain more than a single chromosome at all times of the cell cycle. Heterogeneity of chromosome copy numbers may reflect different subpopulations having diverging growth rates even during exponential growth conditions. Cells continued to initiate replication and segregate chromosomes after induction of DNA damage, as judged by an increase in origin numbers per cell, showing that replication and segregation are relatively robust against cell cycle perturbation.
Keywords
Bacterial cell cycle, chromosome segregation, Bacillus subtilis, single cell analyses
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CURRENT STATUS: Published
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Posted 22 Sep, 2020
Published
On 09 Oct, 2020
No community comments so far
Editorial decision: Accept
On 29 Sep, 2020
Review #1 received
Received 27 Sep, 2020
Reviewers invited
Invitations sent on 21 Sep, 2020
Reviewer #1 agreed
On 21 Sep, 2020
Editor assigned
On 18 Sep, 2020
Submission checks complete
On 17 Sep, 2020
Editor invited
On 17 Sep, 2020
No comments provided
Editorial decision: Major revision
On 09 Sep, 2020
Review #1 received
Received 04 Sep, 2020
Reviewer #1 agreed
On 24 Aug, 2020
Reviewers invited
Invitations sent on 21 Aug, 2020
Submission checks complete
On 14 Aug, 2020
Editor invited
On 13 Aug, 2020
Editor assigned
On 12 Aug, 2020
First submitted
On 11 Aug, 2020
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Subject Areas
Cell Cycle & Proliferation
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This preprint is under consideration at BMC Research Notes. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research note
Chromosome segregation in B. subtilis is highly heterogeneous
Peter Graumann
Philipps-Universitat Marburg
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8033-5171
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 22 Sep, 2020
Published
On 09 Oct, 2020
No community comments so far
Editorial decision: Accept
On 29 Sep, 2020
Review #1 received
Received 27 Sep, 2020
Reviewers invited
Invitations sent on 21 Sep, 2020
Reviewer #1 agreed
On 21 Sep, 2020
Editor assigned
On 18 Sep, 2020
Submission checks complete
On 17 Sep, 2020
Editor invited
On 17 Sep, 2020
No comments provided
Editorial decision: Major revision
On 09 Sep, 2020
Review #1 received
Received 04 Sep, 2020
Reviewer #1 agreed
On 24 Aug, 2020
Reviewers invited
Invitations sent on 21 Aug, 2020
Submission checks complete
On 14 Aug, 2020
Editor invited
On 13 Aug, 2020
Editor assigned
On 12 Aug, 2020
First submitted
On 11 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cell Cycle & Proliferation
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Research Notes.
Objective: The bacterial cell cycle comprises initiation of replication and ensuing elongation, concomitant chromosome segregation (in some organisms with a delay termed cohesion), and finally cell division. By quantifying the number of origin and terminus regions in exponentially growing Bacillus subtilis cells, and after induction of DNA damage, we aimed at determining cell cycle parameters at different growth rates at a single cell level.
Results: B. subtilis cells are mostly mero-oligoploid during fast growth and diploid during slow growth. However, we found that the number of replication origins and of termini is highly heterogeneous within the cell population at two different growth rates, and that even at slow growth, a majority of cells attempts to maintain more than a single chromosome at all times of the cell cycle. Heterogeneity of chromosome copy numbers may reflect different subpopulations having diverging growth rates even during exponential growth conditions. Cells continued to initiate replication and segregate chromosomes after induction of DNA damage, as judged by an increase in origin numbers per cell, showing that replication and segregation are relatively robust against cell cycle perturbation.
Figure 1
Figure 2