See the published version of this preprint at Journal of Ovarian Research.
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Research
Johannes Ott
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2761-5262
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Background
Vitrification has superseded the slow freezing method for cryopreservation of oocytes, embryos, and sperm, but there are yet no standard protocols for its use in ovarian tissue cryopreservation (OTC). Published protocols diverse mainly in the supplementation of dimethyl sulfoxide (DMSO) to the vitrification medium and the use of an open or closed vitrification system.
We investigated the vitality of ovarian tissue of transgender patients by Fluorescence Activated Cells Sorting (FACS) and histomorphological analyses using a DMSO-containing (P1) and a DMSO-free protocol (P2) in an open or closed vitrification setting.
Results
Twelve ovarian samples were donated from female-to-male transgender patients: 6 were vitrified according to protocol 1, the other 6 according to protocol 2. The amount of vital cells was 90.1% (P1) and 88.4% (P2) before vitrification. After vitrification and subsequent warming, vital cells were reduced to 82.9% (P1, p=0.093) and 72.4% (P2, p=0.019). When comparing the closed and the open systems, the decline in cell vitality from pre- to post-vitrification was significant only for the latter (p= 0.037). Histological examination reveals no significant differences with respect to degenerated follicles before or after vitrification.
Conclusion
These results lend support to the hypothesis that a protocol containing DMSO results in a higher vitality of ovarian cells than a protocol that uses ethylene glycol as cryoprotective agent in vitrification. The use of an open vitrification system led to significant decline in the rate of vital cells.
Trial registration: NCT03649087, retrospectively registered 28.08.2018
Keywords
Vitrification, fertility preservation, ovarian tissue cryopreservation, cell vitality
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View the published article at Journal of Ovarian Research.
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Editorial decision: Major revision
On 02 Sep, 2021
Review #2 received
Received 31 Aug, 2021
Reviewer #2 agreed
On 17 Aug, 2021
Review #1 received
Received 18 Jun, 2021
Reviewer #1 agreed
On 15 Jun, 2021
Reviews received
Received 15 Jun, 2021
Reviewers invited
Invitations sent on 08 Jun, 2021
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On 31 May, 2021
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On 30 May, 2021
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Journal of Ovarian Research.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Johannes Ott
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2761-5262
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of Ovarian Research.
Version 1
Posted 09 Jun, 2021
No community comments so far
Editorial decision: Major revision
On 02 Sep, 2021
Review #2 received
Received 31 Aug, 2021
Reviewer #2 agreed
On 17 Aug, 2021
Review #1 received
Received 18 Jun, 2021
Reviewer #1 agreed
On 15 Jun, 2021
Reviews received
Received 15 Jun, 2021
Reviewers invited
Invitations sent on 08 Jun, 2021
Editor assigned
On 31 May, 2021
First submitted
On 30 May, 2021
Submission checks complete
On 30 May, 2021
Editor invited
On 30 May, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of Ovarian Research.
Background
Vitrification has superseded the slow freezing method for cryopreservation of oocytes, embryos, and sperm, but there are yet no standard protocols for its use in ovarian tissue cryopreservation (OTC). Published protocols diverse mainly in the supplementation of dimethyl sulfoxide (DMSO) to the vitrification medium and the use of an open or closed vitrification system.
We investigated the vitality of ovarian tissue of transgender patients by Fluorescence Activated Cells Sorting (FACS) and histomorphological analyses using a DMSO-containing (P1) and a DMSO-free protocol (P2) in an open or closed vitrification setting.
Results
Twelve ovarian samples were donated from female-to-male transgender patients: 6 were vitrified according to protocol 1, the other 6 according to protocol 2. The amount of vital cells was 90.1% (P1) and 88.4% (P2) before vitrification. After vitrification and subsequent warming, vital cells were reduced to 82.9% (P1, p=0.093) and 72.4% (P2, p=0.019). When comparing the closed and the open systems, the decline in cell vitality from pre- to post-vitrification was significant only for the latter (p= 0.037). Histological examination reveals no significant differences with respect to degenerated follicles before or after vitrification.
Conclusion
These results lend support to the hypothesis that a protocol containing DMSO results in a higher vitality of ovarian cells than a protocol that uses ethylene glycol as cryoprotective agent in vitrification. The use of an open vitrification system led to significant decline in the rate of vital cells.
Trial registration: NCT03649087, retrospectively registered 28.08.2018
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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