This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research article
Kishor Chand Kumhar
Chaudhary Charan Singh Haryana Agricultural University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: The dieback disease of tea caused by Fusariumsolani adversely affects its production and quality. Genus Trichoderma is a promising biocontrol agent to control it without any residual effect and most suitable for inclusion into an integrated disease management approach. Isolation of Trichoderma from rhizosphere soil of the Dooars zone was done. It was identified as T. asperellum based on its cultural characters and DNA fingerprinting. A liquid formulation (2% Aqueous Suspension) was prepared from it and bio-efficacy was evaluated under field conditions for the control of dieback disease and other parameters for two seasons in three zones. Its different concentrations, T. harzianum, and Hexaconazole were included in the study.
Results: The fungal isolate (KBN-29), identified as T. asperellum, was found nearest to the isolate TV-3 (Genebank-KX538814.1) with 99% similarity. Plots treated with T. asperellum 2% AS at 1000 and 1200ml/ha concentration gave better disease control as the yield of green leaves as compared to Hexaconazole 5% EC. The formulation was safe to non-target beneficial organisms’ in all three zones without any phytotoxicity to tea leaves at 4, 8, and 16ml/L concentrations.
Conclusions: The present study confirms that the developed liquid formulation of T. asperellum 2% AS was found significantly superior for the management of dieback disease of tea plantations in Darjeeling, Dooars, and Assam zones when used at concentrations of 1200 and 1000 ml followed by 800 and 600 ml/ha during both seasons. The maximum made tea yield was recorded in plots treated with T. asperellum at 1000 and 1200ml/ha followed by Hexaconazole 5% EC. The T. asperellum 2% AS was observed to be safe for non-target beneficial organisms viz., C. carnea, O. javanus, and S. gilvifrons. The developed T. asperellum 2% AS formulation was not phytotoxic to tea leaves at 4, 8, and 16ml/L concentrations.
Keywords
Trichoderma asperellum 2% AS, tea, dieback, yield, non-target organisms, phytotoxicity
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
The most recent version of this article is available here.
No community comments so far
No comments provided
Version 1
Posted 03 Sep, 2020
Community comments: 1
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Kishor Chand Kumhar
Chaudhary Charan Singh Haryana Agricultural University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
The most recent version of this article is available here.
No community comments so far
No comments provided
Version 1
Posted 03 Sep, 2020
Community comments: 1
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
Background: The dieback disease of tea caused by Fusariumsolani adversely affects its production and quality. Genus Trichoderma is a promising biocontrol agent to control it without any residual effect and most suitable for inclusion into an integrated disease management approach. Isolation of Trichoderma from rhizosphere soil of the Dooars zone was done. It was identified as T. asperellum based on its cultural characters and DNA fingerprinting. A liquid formulation (2% Aqueous Suspension) was prepared from it and bio-efficacy was evaluated under field conditions for the control of dieback disease and other parameters for two seasons in three zones. Its different concentrations, T. harzianum, and Hexaconazole were included in the study.
Results: The fungal isolate (KBN-29), identified as T. asperellum, was found nearest to the isolate TV-3 (Genebank-KX538814.1) with 99% similarity. Plots treated with T. asperellum 2% AS at 1000 and 1200ml/ha concentration gave better disease control as the yield of green leaves as compared to Hexaconazole 5% EC. The formulation was safe to non-target beneficial organisms’ in all three zones without any phytotoxicity to tea leaves at 4, 8, and 16ml/L concentrations.
Conclusions: The present study confirms that the developed liquid formulation of T. asperellum 2% AS was found significantly superior for the management of dieback disease of tea plantations in Darjeeling, Dooars, and Assam zones when used at concentrations of 1200 and 1000 ml followed by 800 and 600 ml/ha during both seasons. The maximum made tea yield was recorded in plots treated with T. asperellum at 1000 and 1200ml/ha followed by Hexaconazole 5% EC. The T. asperellum 2% AS was observed to be safe for non-target beneficial organisms viz., C. carnea, O. javanus, and S. gilvifrons. The developed T. asperellum 2% AS formulation was not phytotoxic to tea leaves at 4, 8, and 16ml/L concentrations.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Loading...