This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Primary research
Tongzu Liu
Zhongnan Hospital of Wuhan University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9568-9036
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Prostate cancer is one of the common cancers among males with high incidence and mortality. MiR-103 has been reported to be involved in several human cancers. In our study, we aimed to explore the diagnostic value of miR-103 in PCa.
Methods: In this study, qRT-PCR was performed to investigate the expression levels of miR-103 in serum specimens collected from 141 PCa patients and 77 healthy volunteers. MTT and cell invasion assay were performed to detect the effects of miR-103 on the proliferation and invasion of PCa cells. The diagnostic value of miR-103 in PCa was evaluated by ROC curve.
Results: The results showed that serum miR-103 levels were higher in PCa patients than that in healthy group (P<0.001). And the expression of miR-103 was significantly associated with PSA (P=0.007), differentiation (P=0.009), and lymph node metastasis (P=0.037). Cell experiments in vitro demonstrated that miR-103 could promote PCa cell line proliferation and invasion. In addition, ROC analysis suggested that miR-103 could discriminate between PCa patients and healthy individuals at the cut-off value of 0.970. The AUC was 0.885, with the sensitivity of 97.2% and specificity of 81.8%.
Conclusions: The increased miR-103 levels can promote proliferation and invasion of PCa cells. Serum miR-103 is significantly associated with tumor progression, which may be a novel biomarker for PCa diagnosis.
Keywords
MiR-103, Prostate cancer, Diagnosis
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Primary research
Tongzu Liu
Zhongnan Hospital of Wuhan University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9568-9036
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 17 Aug, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Prostate cancer is one of the common cancers among males with high incidence and mortality. MiR-103 has been reported to be involved in several human cancers. In our study, we aimed to explore the diagnostic value of miR-103 in PCa.
Methods: In this study, qRT-PCR was performed to investigate the expression levels of miR-103 in serum specimens collected from 141 PCa patients and 77 healthy volunteers. MTT and cell invasion assay were performed to detect the effects of miR-103 on the proliferation and invasion of PCa cells. The diagnostic value of miR-103 in PCa was evaluated by ROC curve.
Results: The results showed that serum miR-103 levels were higher in PCa patients than that in healthy group (P<0.001). And the expression of miR-103 was significantly associated with PSA (P=0.007), differentiation (P=0.009), and lymph node metastasis (P=0.037). Cell experiments in vitro demonstrated that miR-103 could promote PCa cell line proliferation and invasion. In addition, ROC analysis suggested that miR-103 could discriminate between PCa patients and healthy individuals at the cut-off value of 0.970. The AUC was 0.885, with the sensitivity of 97.2% and specificity of 81.8%.
Conclusions: The increased miR-103 levels can promote proliferation and invasion of PCa cells. Serum miR-103 is significantly associated with tumor progression, which may be a novel biomarker for PCa diagnosis.
Figure 1
Figure 2
Figure 3
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