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Research Article
Alagesan Chinnasamy
Independent Researcher
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6432-7569
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This work is licensed under a CC BY 4.0 License. Read Full License
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- The author has confirmed that a statement listing potential conflicts of interest or lack thereof is included in the text.
Although oral cancer is multifactorial in origin only few had evaluated the diagnostic parameters for candidal infection in oral squamous cell carcinoma (OSCC). We aims to compare and assess the accuracy of Calcofluor Whilte (CFW) to that of conventional staining methods to identify the presence of candidal infection in OSCC. Archival collection of 43 OSCC were retrieved for this investigation. Standard staining protocol was followed for the index test (CFW) and reference standard (Periodic Acid Schiff). Two examiners were blinded for either one of the staining techniques. Diagnostic test evaluation and Kappa statistic was calculated using MedCalc software. The study comprised of 37 males, six females, and their mean age at the time of presentation was 51 (range 23 to 75 years). The sensitivity and specificity of CFW was 75.8% (CI 57.7 to 88.9%) and 10.0% (CI 0.2 to 44.5%). While the positive predictive and negative predictive values were 63.1% (CI − 50.6% − 74.0%) and 67.0% (CI − 49.2% − 81.0%). Our results show minimal agreement with PAS with a Kappa score of 0.148 (95% CI = 0.39 to 0.09). Overall detection rate was similar for both the index test and reference standard although there was considerable number of intermediate test results. Sensitivity of CFW was comparable to that of earlier studies but specificity was low and the degree of concordance of 60.4%. Although CFW staining can come with high false positive they are an useful test for ruling out candida infection when the test result is negative.
Keywords
candida albicans, periodic acid Schiff, calcofluor white, leukoplakia
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This is a preprint. It has not completed peer review.
Research Article
Alagesan Chinnasamy
Independent Researcher
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6432-7569
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 04 Jun, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Declarations:
Declarations
- The author has confirmed that a statement listing potential conflicts of interest or lack thereof is included in the text.
Although oral cancer is multifactorial in origin only few had evaluated the diagnostic parameters for candidal infection in oral squamous cell carcinoma (OSCC). We aims to compare and assess the accuracy of Calcofluor Whilte (CFW) to that of conventional staining methods to identify the presence of candidal infection in OSCC. Archival collection of 43 OSCC were retrieved for this investigation. Standard staining protocol was followed for the index test (CFW) and reference standard (Periodic Acid Schiff). Two examiners were blinded for either one of the staining techniques. Diagnostic test evaluation and Kappa statistic was calculated using MedCalc software. The study comprised of 37 males, six females, and their mean age at the time of presentation was 51 (range 23 to 75 years). The sensitivity and specificity of CFW was 75.8% (CI 57.7 to 88.9%) and 10.0% (CI 0.2 to 44.5%). While the positive predictive and negative predictive values were 63.1% (CI − 50.6% − 74.0%) and 67.0% (CI − 49.2% − 81.0%). Our results show minimal agreement with PAS with a Kappa score of 0.148 (95% CI = 0.39 to 0.09). Overall detection rate was similar for both the index test and reference standard although there was considerable number of intermediate test results. Sensitivity of CFW was comparable to that of earlier studies but specificity was low and the degree of concordance of 60.4%. Although CFW staining can come with high false positive they are an useful test for ruling out candida infection when the test result is negative.
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