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Research Article
De He
Shenzhen Bao'an District People's Hospital
Corresponding Author
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LINC 01436 (lncRNA) promotes lung and gastric two types of cancers. However, it is unclear that whether this lncRNA also participate in colorectal cancer (CRC). This study was therefore carried out to analyze the role of LINC 01436 in CRC. Expression of LINC 01436 in CRC patient tissues was analyzed by RT-qPCR and follow-up study was performed for prognostic analysis. Correlation between LINC 01436 and mature miR-466 or miR-466 precursor was analyzed by linear regression. Mature miR-466 and miR-466 precursor expression in CRC cells with the overexpression of LINC 01436 was studied by performing RT-qPCR. The proliferation of CRC cells was subjected to CCK-8 assay analysis. LINC 01436 was upregulated in CRC and predicted poor survival. LINC 01436 and mature miR-466 were inversely correlated, but LINC 01436 and miR-466 precursor were not correlated. In CRC cells, LINC 01436 mediated the downregulation of mature miR-466, but not miR-466 precursor. Cell proliferation analysis showed that LINC 01436 overexpression rescued cell proliferation reduced by miR-466. LINC 01436 is overexpressed in CRC and it may promote cancer cell proliferation by suppressing the maturation of miR-466.
Keywords
colorectal cancer, LINC 01436, miR-466, maturation
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No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementalTable.1.docx
Supplemental Table.1 Sequences of primers used in RT-qPCR.
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
De He
Shenzhen Bao'an District People's Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 10 Jun, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Oncology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
LINC 01436 (lncRNA) promotes lung and gastric two types of cancers. However, it is unclear that whether this lncRNA also participate in colorectal cancer (CRC). This study was therefore carried out to analyze the role of LINC 01436 in CRC. Expression of LINC 01436 in CRC patient tissues was analyzed by RT-qPCR and follow-up study was performed for prognostic analysis. Correlation between LINC 01436 and mature miR-466 or miR-466 precursor was analyzed by linear regression. Mature miR-466 and miR-466 precursor expression in CRC cells with the overexpression of LINC 01436 was studied by performing RT-qPCR. The proliferation of CRC cells was subjected to CCK-8 assay analysis. LINC 01436 was upregulated in CRC and predicted poor survival. LINC 01436 and mature miR-466 were inversely correlated, but LINC 01436 and miR-466 precursor were not correlated. In CRC cells, LINC 01436 mediated the downregulation of mature miR-466, but not miR-466 precursor. Cell proliferation analysis showed that LINC 01436 overexpression rescued cell proliferation reduced by miR-466. LINC 01436 is overexpressed in CRC and it may promote cancer cell proliferation by suppressing the maturation of miR-466.
Figure 1
Figure 2
Figure 3
Figure 4
No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementalTable.1.docx
Supplemental Table.1 Sequences of primers used in RT-qPCR.
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