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Research article
Dequan Xu
Huazhong Agriculture University
Corresponding Author
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Background: MicroRNAs (miRNAs) are small non-coding RNAs playing vital roles in regulating posttranscriptional gene expression. Elucidating the expression regulation of miRNAs underlying pig testis development will contribute to a better understanding of boar fertility and spermatogenesis.
Results: In this study, miRNA expression profile was investigated in testes of Duroc and Meishan boars at 20, 75, and 270 days of age by high-throughput sequencing. Forty-five differentially expressed miRNAs were identified from testes of Duroc and Meishan boars before and after puberty. Integrated analysis of miRNA and mRNA profiles predicted many miRNA-mRNA pairs. Gene ontology and biological pathway analyses revealed that predicted target genes of ssc-mir-423-5p, ssc-mir-34c, ssc-mir-107, ssc-165 mir-196b-5p, ssc-mir-92a, ssc-mir-320, ssc-mir-10a-5p, and ssc-mir-181b were involved in sexual reproduction, male gamete generation, and spermatogenesis, and GnRH, Wnt, and MAPK signaling pathway. Four significantly differentially expressed miRNAs and their predicted target genes were validated by quantitative real-time polymerase chain reaction, and phospholipase C beta 1 ( PLCĪ²1) gene was verified to be a target of ssc-mir-423-5p .
Conclusions: This study provides an insight into the functional roles of miRNAs in testis development and spermatogenesis and offers useful resources for understanding differences in sexual function development caused by the change in miRNAs expression between Duroc and Meishan boars.
Keywords
Meishan boar, Duroc boar, Testis, Sexual development, miRNA, Integrating analysis
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Editorial decision: Minor revision
On 09 Sep, 2020
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On 07 Sep, 2020
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On 06 Sep, 2020
Editor invited
On 06 Sep, 2020
No comments provided
Submission checks complete
On 03 Sep, 2020
Editorial decision: Minor revision
On 03 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 12 Aug, 2020
Reviewer #2 agreed
On 25 Apr, 2020
Reviewer #1 agreed
On 23 Apr, 2020
Review #1 received
Received 23 Apr, 2020
Editor assigned
On 20 Apr, 2020
Reviewers invited
Invitations sent on 20 Apr, 2020
Submission checks complete
On 19 Apr, 2020
Editor invited
On 19 Apr, 2020
Version 1
Posted 30 Sep, 2019
No comments provided
Editorial decision: Major revision
On 20 Mar, 2020
Review #2 received
Received 11 Mar, 2020
Reviewer #2 agreed
On 24 Feb, 2020
Review #1 received
Received 01 Feb, 2020
Reviewer #1 agreed
On 21 Nov, 2019
Reviewers invited
Invitations sent on 16 Nov, 2019
Submission checks complete
On 23 Sep, 2019
Editor assigned
On 05 Sep, 2019
First submitted
On 04 Sep, 2019
Editor invited
On 04 Sep, 2019
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Subject Areas
Epigenetics & Genomics
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A new preprint design is coming!
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See the published version of this preprint at BMC Genomics.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Dequan Xu
Huazhong Agriculture University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Genomics.
No community comments so far
Submission checks complete
On 27 Sep, 2020
Editorial decision: Accept
On 23 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 18 Sep, 2020
Editor assigned
On 14 Sep, 2020
Submission checks complete
On 13 Sep, 2020
Editor invited
On 13 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 09 Sep, 2020
Editor assigned
On 07 Sep, 2020
Submission checks complete
On 06 Sep, 2020
Editor invited
On 06 Sep, 2020
No comments provided
Submission checks complete
On 03 Sep, 2020
Editorial decision: Minor revision
On 03 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 12 Aug, 2020
Reviewer #2 agreed
On 25 Apr, 2020
Reviewer #1 agreed
On 23 Apr, 2020
Review #1 received
Received 23 Apr, 2020
Editor assigned
On 20 Apr, 2020
Reviewers invited
Invitations sent on 20 Apr, 2020
Submission checks complete
On 19 Apr, 2020
Editor invited
On 19 Apr, 2020
Version 1
Posted 30 Sep, 2019
No comments provided
Editorial decision: Major revision
On 20 Mar, 2020
Review #2 received
Received 11 Mar, 2020
Reviewer #2 agreed
On 24 Feb, 2020
Review #1 received
Received 01 Feb, 2020
Reviewer #1 agreed
On 21 Nov, 2019
Reviewers invited
Invitations sent on 16 Nov, 2019
Submission checks complete
On 23 Sep, 2019
Editor assigned
On 05 Sep, 2019
First submitted
On 04 Sep, 2019
Editor invited
On 04 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Genomics.
Background: MicroRNAs (miRNAs) are small non-coding RNAs playing vital roles in regulating posttranscriptional gene expression. Elucidating the expression regulation of miRNAs underlying pig testis development will contribute to a better understanding of boar fertility and spermatogenesis.
Results: In this study, miRNA expression profile was investigated in testes of Duroc and Meishan boars at 20, 75, and 270 days of age by high-throughput sequencing. Forty-five differentially expressed miRNAs were identified from testes of Duroc and Meishan boars before and after puberty. Integrated analysis of miRNA and mRNA profiles predicted many miRNA-mRNA pairs. Gene ontology and biological pathway analyses revealed that predicted target genes of ssc-mir-423-5p, ssc-mir-34c, ssc-mir-107, ssc-165 mir-196b-5p, ssc-mir-92a, ssc-mir-320, ssc-mir-10a-5p, and ssc-mir-181b were involved in sexual reproduction, male gamete generation, and spermatogenesis, and GnRH, Wnt, and MAPK signaling pathway. Four significantly differentially expressed miRNAs and their predicted target genes were validated by quantitative real-time polymerase chain reaction, and phospholipase C beta 1 ( PLCĪ²1) gene was verified to be a target of ssc-mir-423-5p .
Conclusions: This study provides an insight into the functional roles of miRNAs in testis development and spermatogenesis and offers useful resources for understanding differences in sexual function development caused by the change in miRNAs expression between Duroc and Meishan boars.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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