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Research article
Javier Caviedes-Bucheli
Pontificia Universidad Javeriana
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0407-9847
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background To quantify the expression of SP, CGRP, and VEGF in human dental pulps as a response to occlusal interferences, moderate orthodontic forces, and occlusal interferences plus moderate orthodontic forces simultaneously.
Methods Forty human dental pulp samples were collected from healthy premolars indicated for extraction for orthodontic reasons. The teeth were divided into four groups with 10 samples each: Healthy pulp control group, occlusal interference group, moderate orthodontic forces group; and occlusal interferences plus moderate orthodontic forces group. Stimuli were applied for 24 h before tooth extraction in all experimental groups. All samples were processed, and SP, CGRP, and VEGF were measured by radioimmunoassay. The ANOVA test was performed to establish significant differences between groups and Tukey’s HSD post hoc comparisons were also performed.
Results The highest increase in SP, CGRP, and VEGF expressions was found in the occlusal trauma plus orthodontic forces group, followed by the moderate orthodontic forces, the occlusal interferences and the control groups, with statistically significant differences between all groups (ANOVA p<0.001) and post-hoc comparisons (Tukey HSD p<0.001).
Conclusion SP, CGRP, and VEGF expressions significantly increase in human dental pulps when stimulated by occlusal interferences combined with moderate orthodontic forces, as compared with when these two stimuli are applied independently. Peptides expression is directly proportional to the magnitude of the stimulus.
Name of the registry: Importance of Neurogenic Inflammation in the Angiogenic Response of the Dental Pulp as a Defensive Response
Trial registration number: NCT03804034
Date of registration: 01/15/2019 Retrospectively registered
URL of trial registry record: https://clinicaltrials.gov/ct2/show/NCT03804034?term=NCT03804034&draw=2&rank=1
Keywords
SP, CGRP, VEGF, oclusal trauma, orthodontics forces, neurogenic inflammation
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Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Oral Health.
No community comments so far
Editorial decision: Major revision
On 10 Jan, 2021
Review #2 received
Received 22 Dec, 2020
Review #1 received
Received 22 Dec, 2020
Reviewer #2 agreed
On 11 Dec, 2020
Reviewer #1 agreed
On 11 Dec, 2020
Reviewers invited
Invitations sent on 08 Dec, 2020
Editor assigned
On 26 Nov, 2020
Submission checks complete
On 26 Nov, 2020
Editor invited
On 26 Nov, 2020
Version 1
Posted 09 Sep, 2020
No comments provided
Editorial decision: Major revision
On 01 Nov, 2020
Review #2 received
Received 13 Oct, 2020
Review #1 received
Received 11 Oct, 2020
Reviewer #2 agreed
On 24 Sep, 2020
Reviewer #1 agreed
On 22 Sep, 2020
Reviewers invited
Invitations sent on 21 Sep, 2020
First submitted
On 18 Aug, 2020
Editor assigned
On 18 Aug, 2020
Submission checks complete
On 17 Aug, 2020
Editor invited
On 17 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Dentistry
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A new preprint design is coming!
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See the published version of this preprint at BMC Oral Health.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Javier Caviedes-Bucheli
Pontificia Universidad Javeriana
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0407-9847
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Oral Health.
No community comments so far
Editorial decision: Major revision
On 10 Jan, 2021
Review #2 received
Received 22 Dec, 2020
Review #1 received
Received 22 Dec, 2020
Reviewer #2 agreed
On 11 Dec, 2020
Reviewer #1 agreed
On 11 Dec, 2020
Reviewers invited
Invitations sent on 08 Dec, 2020
Editor assigned
On 26 Nov, 2020
Submission checks complete
On 26 Nov, 2020
Editor invited
On 26 Nov, 2020
Version 1
Posted 09 Sep, 2020
No comments provided
Editorial decision: Major revision
On 01 Nov, 2020
Review #2 received
Received 13 Oct, 2020
Review #1 received
Received 11 Oct, 2020
Reviewer #2 agreed
On 24 Sep, 2020
Reviewer #1 agreed
On 22 Sep, 2020
Reviewers invited
Invitations sent on 21 Sep, 2020
First submitted
On 18 Aug, 2020
Editor assigned
On 18 Aug, 2020
Submission checks complete
On 17 Aug, 2020
Editor invited
On 17 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Dentistry
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Oral Health.
Background To quantify the expression of SP, CGRP, and VEGF in human dental pulps as a response to occlusal interferences, moderate orthodontic forces, and occlusal interferences plus moderate orthodontic forces simultaneously.
Methods Forty human dental pulp samples were collected from healthy premolars indicated for extraction for orthodontic reasons. The teeth were divided into four groups with 10 samples each: Healthy pulp control group, occlusal interference group, moderate orthodontic forces group; and occlusal interferences plus moderate orthodontic forces group. Stimuli were applied for 24 h before tooth extraction in all experimental groups. All samples were processed, and SP, CGRP, and VEGF were measured by radioimmunoassay. The ANOVA test was performed to establish significant differences between groups and Tukey’s HSD post hoc comparisons were also performed.
Results The highest increase in SP, CGRP, and VEGF expressions was found in the occlusal trauma plus orthodontic forces group, followed by the moderate orthodontic forces, the occlusal interferences and the control groups, with statistically significant differences between all groups (ANOVA p<0.001) and post-hoc comparisons (Tukey HSD p<0.001).
Conclusion SP, CGRP, and VEGF expressions significantly increase in human dental pulps when stimulated by occlusal interferences combined with moderate orthodontic forces, as compared with when these two stimuli are applied independently. Peptides expression is directly proportional to the magnitude of the stimulus.
Name of the registry: Importance of Neurogenic Inflammation in the Angiogenic Response of the Dental Pulp as a Defensive Response
Trial registration number: NCT03804034
Date of registration: 01/15/2019 Retrospectively registered
URL of trial registry record: https://clinicaltrials.gov/ct2/show/NCT03804034?term=NCT03804034&draw=2&rank=1
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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