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Research article
Marian Taulescu
Universitatea de Medicina si Farmacie Iuliu Hatieganu
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8987-6038
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This work is licensed under a CC BY 4.0 License. Read Full License
Ovine pulmonary adenocarcinoma (OPA) is a neoplastic disease caused by exogenous Jaagsiekte Sheep Retrovirus (exJSRV). The prevalence of JSRV-related OPA in Eastern European countries, including Romania is unknown. We aimed to investigate: the prevalence and morphological features of OPA (classical and atypical forms) in Romania, the immunophenotype of the pulmonary tumors and their relationships with exJSRV infection. Lung tumors from 28 slaughtered adult sheep and 6 necropsied individuals were grossly evaluated. Cytology, histology, immunocytochemistry, immunohistochemistry, electron microscopy and DNA testing were subsequently performed. Out of 2693 examined sheep, 34 had OPA (1.26% prevalence). The diaphragmatic lobe was the most affected. Grossly, the classical OPA was identified in 88.24% of investigated cases and the atypical OPA in 11.76% that included solitary myxomatous nodules. Histopathology results confirmed the presence of OPA in all suspected cases, which were classified into acinar and papillary types. Myxoid growths (MGs) were diagnosed in 6 classical OPA cases and in 2 cases of atypical form. JSRV-MA was identified by IHC (94.11%) in both epithelial and mesenchymal cells of OPA. Immunocytochemistry and electron microscopy also confirmed the JSRV within the neoplastic cells. Adenocarcinoma was positive for MCK and TTF-1, and MGs showed immunoreaction for Vimentin, Desmin and SMA; Ki67 expression of classical OPA was higher than atypical OPA and MGs. Phylogenetic analysis revealed the presence of the exJSRV type 2 in Romanian sheep affected by lung cancer and showed a high similarity with the UK strain (AF105220.1), confirming exJSRV2 for the first time in Romania. Additionally, we described the first report of atypical OPA in Romania, and to the best of our knowledge, in Eastern Europe. Finally, we showed that MGs have a myofibroblastic origin.
Keywords
Atypical OPA; Epidemiology; Jaagsiekte Sheep Retrovirus type 2; Myxoid growths; Ovine pulmonary adenocarcinoma.
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CURRENT STATUS: Published
View the published article at BMC Veterinary Research.
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Editorial decision: Minor revision
On 17 Jul, 2020
Review #1 received
Received 05 Apr, 2020
Reviewer #1 agreed
On 22 Mar, 2020
Editor assigned
On 20 Mar, 2020
Reviewers invited
Invitations sent on 20 Mar, 2020
Submission checks complete
On 19 Mar, 2020
Editor invited
On 19 Mar, 2020
Version 1
Posted 03 Oct, 2019
No comments provided
Editorial decision: Major revision
On 26 Feb, 2020
Review #3 received
Received 10 Feb, 2020
Review #2 received
Received 03 Feb, 2020
Reviewer #2 agreed
On 14 Jan, 2020
Reviewer #3 agreed
On 14 Jan, 2020
Review #1 received
Received 29 Dec, 2019
Reviewer #1 agreed
On 16 Dec, 2019
Reviewers invited
Invitations sent on 11 Oct, 2019
Submission checks complete
On 27 Sep, 2019
Editor assigned
On 26 Sep, 2019
Editor invited
On 25 Sep, 2019
First submitted
On 24 Sep, 2019
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See the published version of this preprint at BMC Veterinary Research.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Marian Taulescu
Universitatea de Medicina si Farmacie Iuliu Hatieganu
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8987-6038
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Veterinary Research.
No community comments so far
Editorial decision: Minor revision
On 17 Jul, 2020
Review #1 received
Received 05 Apr, 2020
Reviewer #1 agreed
On 22 Mar, 2020
Editor assigned
On 20 Mar, 2020
Reviewers invited
Invitations sent on 20 Mar, 2020
Submission checks complete
On 19 Mar, 2020
Editor invited
On 19 Mar, 2020
Version 1
Posted 03 Oct, 2019
No comments provided
Editorial decision: Major revision
On 26 Feb, 2020
Review #3 received
Received 10 Feb, 2020
Review #2 received
Received 03 Feb, 2020
Reviewer #2 agreed
On 14 Jan, 2020
Reviewer #3 agreed
On 14 Jan, 2020
Review #1 received
Received 29 Dec, 2019
Reviewer #1 agreed
On 16 Dec, 2019
Reviewers invited
Invitations sent on 11 Oct, 2019
Submission checks complete
On 27 Sep, 2019
Editor assigned
On 26 Sep, 2019
Editor invited
On 25 Sep, 2019
First submitted
On 24 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Veterinary Research.
Ovine pulmonary adenocarcinoma (OPA) is a neoplastic disease caused by exogenous Jaagsiekte Sheep Retrovirus (exJSRV). The prevalence of JSRV-related OPA in Eastern European countries, including Romania is unknown. We aimed to investigate: the prevalence and morphological features of OPA (classical and atypical forms) in Romania, the immunophenotype of the pulmonary tumors and their relationships with exJSRV infection. Lung tumors from 28 slaughtered adult sheep and 6 necropsied individuals were grossly evaluated. Cytology, histology, immunocytochemistry, immunohistochemistry, electron microscopy and DNA testing were subsequently performed. Out of 2693 examined sheep, 34 had OPA (1.26% prevalence). The diaphragmatic lobe was the most affected. Grossly, the classical OPA was identified in 88.24% of investigated cases and the atypical OPA in 11.76% that included solitary myxomatous nodules. Histopathology results confirmed the presence of OPA in all suspected cases, which were classified into acinar and papillary types. Myxoid growths (MGs) were diagnosed in 6 classical OPA cases and in 2 cases of atypical form. JSRV-MA was identified by IHC (94.11%) in both epithelial and mesenchymal cells of OPA. Immunocytochemistry and electron microscopy also confirmed the JSRV within the neoplastic cells. Adenocarcinoma was positive for MCK and TTF-1, and MGs showed immunoreaction for Vimentin, Desmin and SMA; Ki67 expression of classical OPA was higher than atypical OPA and MGs. Phylogenetic analysis revealed the presence of the exJSRV type 2 in Romanian sheep affected by lung cancer and showed a high similarity with the UK strain (AF105220.1), confirming exJSRV2 for the first time in Romania. Additionally, we described the first report of atypical OPA in Romania, and to the best of our knowledge, in Eastern Europe. Finally, we showed that MGs have a myofibroblastic origin.
Figure 1
Figure 2
Figure 3
Figure 4
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