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Research article
Xiaohui Li
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ORCiD: https://orcid.org/0000-0003-4650-2295
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Background：To explore the role of PKCγ subunit of rACC neurons in the development of bone cancer pain in rats. Methods：40 adult female SD rats were divided into five groups: blank control group (Naive group), sham operation group (Sham group), bone cancer pain group (BCP group)，empty lentiviral vector group（Vehicle group） and PKCγ/shRNA recombinant lentiviral vector group (PKCγ group). 10μl MADB-106 rat mammary cancer cells suspension was inoculated into the tibia bone marrow cavity of rats in BCP group and Vehicle group similarly. 10μl saline was inoculated into the proximal tibia bone marrow cavity of rats in Sham group. In the PKCγ group, the rats were taken the same treatment as the BCP group, and then 10μl shRNA/PKCγ recombinant lentivirus was injected into the bilateral rACC on the 7th day. The mechanical withdrawal threshold and thermal withdrawal latency were measured every 3 days to assess the rat pain behavior. Immunohistochemistry, Western blotting technology and immunofluorescence were used to detect the expression of PKCγ subunits in rat rACC neurons after operation. Results: From the 3rd day after operation, the mechanical withdrawal thresholds in BCP group, Vehicle group and PKCγ group were significantly decreased than those in Naive group and Sham group (P<0.05). Compared with the BCP group and Vehicle group the mechanical withdrawal threshold in the PKCγ group increased significantly (P<0.05). On the 3rd postoperative day, the thermal withdrawal latency in BCP, Vehicle and PKCγ groups was significantly longer than those in Naive and Sham groups（P＜0.05). From the 14th postoperative day, the TWL in PKCγ group was shorter than that in BCP and Vehicle groups (P<0.05). Western blot analysis showed that the expression of PKCγ in rACC neurons on the 14th day after operation in rats of BCP and Vehicle groups were significantly higher than that in Naive group and Sham group. (P<0.05) However, in the PKCγ group, the expression of PKCγ in rACC neurons was significantly lower than that in BCP group (P<0.05). Conclusion: Up-regulation of PKC subunit of rACC neurons in bone cancer pain rats contributes to the development of pain sensitivity in bone cancer.
Keywords
Bone cancer pain, rat, rACC, PKCγ, gene silencing
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Xiaohui Li
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4650-2295
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
The most recent version of this article is available here.
No community comments so far
No comments provided
No comments provided
Version 2
Posted 11 Mar, 2019
No comments provided
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
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Subject Areas
Internal Medicine Specialties
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
Background：To explore the role of PKCγ subunit of rACC neurons in the development of bone cancer pain in rats. Methods：40 adult female SD rats were divided into five groups: blank control group (Naive group), sham operation group (Sham group), bone cancer pain group (BCP group)，empty lentiviral vector group（Vehicle group） and PKCγ/shRNA recombinant lentiviral vector group (PKCγ group). 10μl MADB-106 rat mammary cancer cells suspension was inoculated into the tibia bone marrow cavity of rats in BCP group and Vehicle group similarly. 10μl saline was inoculated into the proximal tibia bone marrow cavity of rats in Sham group. In the PKCγ group, the rats were taken the same treatment as the BCP group, and then 10μl shRNA/PKCγ recombinant lentivirus was injected into the bilateral rACC on the 7th day. The mechanical withdrawal threshold and thermal withdrawal latency were measured every 3 days to assess the rat pain behavior. Immunohistochemistry, Western blotting technology and immunofluorescence were used to detect the expression of PKCγ subunits in rat rACC neurons after operation. Results: From the 3rd day after operation, the mechanical withdrawal thresholds in BCP group, Vehicle group and PKCγ group were significantly decreased than those in Naive group and Sham group (P<0.05). Compared with the BCP group and Vehicle group the mechanical withdrawal threshold in the PKCγ group increased significantly (P<0.05). On the 3rd postoperative day, the thermal withdrawal latency in BCP, Vehicle and PKCγ groups was significantly longer than those in Naive and Sham groups（P＜0.05). From the 14th postoperative day, the TWL in PKCγ group was shorter than that in BCP and Vehicle groups (P<0.05). Western blot analysis showed that the expression of PKCγ in rACC neurons on the 14th day after operation in rats of BCP and Vehicle groups were significantly higher than that in Naive group and Sham group. (P<0.05) However, in the PKCγ group, the expression of PKCγ in rACC neurons was significantly lower than that in BCP group (P<0.05). Conclusion: Up-regulation of PKC subunit of rACC neurons in bone cancer pain rats contributes to the development of pain sensitivity in bone cancer.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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