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Research article
Shuxun Yu
Chinese Academy of Agricultural Sciences Cotton Research Institute
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9715-3462
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Multiple C2 domains and transmembrane region proteins ( MCTPs ) may act as transport mediators of other regulators. Although increased number of MCTPs in higher plants implies their diverse and specific functions in plant growth and development, only a few plant MCTPs have been studied and no study on the MCTPs in cotton has been reported.
Results: In this study, we identified 31 MCTPs in G. hirsutum , which were classified into five subfamilies according to the phylogenetic analysis. GhMCTPs from subfamily V exhibited pIs less than 7, whereas GhMCTPs from subfamily I, II, III and IV exhibited pIs more than 7.5, implying their distinct biological functions. In addition, GhMCTPs within subfamily III, IV and V exhibited more diverse physicochemical properties, domain architectures and expression patterns than GhMCTPs within subfamily I and II, suggesting that GhMCTPs within subfamily III, IV and V diverged to perform more diverse and specific functions. Analyses of conserved motifs and pIs indicated that the N-terminus was more divergent than the C-terminus and GhMCTPs’ functional divergence might be mainly contributed by the N-terminus. Furthermore, yeast two-hybrid assay indicated that the N-terminus was responsible to interact with target proteins. Phylogenetic analysis classified multiple N-terminal C2 domains into four subclades, suggesting that these C2 domains performed different molecular functions in mediating the transport of target proteins. Conclusions: Our systematic characterization of MCTPs in G. hirsutum will provide helpful information to further research GhMCTPs’ molecular roles in mediating other regulators’ transport to coordinate growth and development of various cotton tissues.
Keywords
G. hirsutum, MCTPs, N-terminus, C-terminus, domain architecture, expression patterns
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CURRENT STATUS: Published
View the published article at BMC Genomics.
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On 16 Jun, 2020
Editorial decision: Accept
On 16 Jun, 2020
Version 3
Posted 12 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 12 Jun, 2020
Editor assigned
On 09 Jun, 2020
Submission checks complete
On 08 Jun, 2020
Editor invited
On 08 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 05 Jun, 2020
Review #1 received
Received 30 May, 2020
Review #2 received
Received 16 May, 2020
Reviewer #2 agreed
On 15 May, 2020
Reviewer #1 agreed
On 13 May, 2020
Editor assigned
On 12 May, 2020
Reviewers invited
Invitations sent on 12 May, 2020
Submission checks complete
On 11 May, 2020
Editor invited
On 11 May, 2020
No comments provided
Editorial decision: Major revision
On 13 Apr, 2020
Review #2 received
Received 06 Apr, 2020
Reviewer #2 agreed
On 16 Mar, 2020
Review #1 received
Received 26 Jan, 2020
Reviewer #1 agreed
On 15 Nov, 2019
Reviewers invited
Invitations sent on 03 Oct, 2019
Submission checks complete
On 27 Sep, 2019
First submitted
On 25 Sep, 2019
Editor assigned
On 25 Sep, 2019
Editor invited
On 24 Sep, 2019
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Subject Areas
Epigenetics & Genomics
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Genomics.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Shuxun Yu
Chinese Academy of Agricultural Sciences Cotton Research Institute
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9715-3462
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Genomics.
No community comments so far
Submission checks complete
On 16 Jun, 2020
Editorial decision: Accept
On 16 Jun, 2020
Version 3
Posted 12 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 12 Jun, 2020
Editor assigned
On 09 Jun, 2020
Submission checks complete
On 08 Jun, 2020
Editor invited
On 08 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 05 Jun, 2020
Review #1 received
Received 30 May, 2020
Review #2 received
Received 16 May, 2020
Reviewer #2 agreed
On 15 May, 2020
Reviewer #1 agreed
On 13 May, 2020
Editor assigned
On 12 May, 2020
Reviewers invited
Invitations sent on 12 May, 2020
Submission checks complete
On 11 May, 2020
Editor invited
On 11 May, 2020
No comments provided
Editorial decision: Major revision
On 13 Apr, 2020
Review #2 received
Received 06 Apr, 2020
Reviewer #2 agreed
On 16 Mar, 2020
Review #1 received
Received 26 Jan, 2020
Reviewer #1 agreed
On 15 Nov, 2019
Reviewers invited
Invitations sent on 03 Oct, 2019
Submission checks complete
On 27 Sep, 2019
First submitted
On 25 Sep, 2019
Editor assigned
On 25 Sep, 2019
Editor invited
On 24 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Genomics.
Background: Multiple C2 domains and transmembrane region proteins ( MCTPs ) may act as transport mediators of other regulators. Although increased number of MCTPs in higher plants implies their diverse and specific functions in plant growth and development, only a few plant MCTPs have been studied and no study on the MCTPs in cotton has been reported.
Results: In this study, we identified 31 MCTPs in G. hirsutum , which were classified into five subfamilies according to the phylogenetic analysis. GhMCTPs from subfamily V exhibited pIs less than 7, whereas GhMCTPs from subfamily I, II, III and IV exhibited pIs more than 7.5, implying their distinct biological functions. In addition, GhMCTPs within subfamily III, IV and V exhibited more diverse physicochemical properties, domain architectures and expression patterns than GhMCTPs within subfamily I and II, suggesting that GhMCTPs within subfamily III, IV and V diverged to perform more diverse and specific functions. Analyses of conserved motifs and pIs indicated that the N-terminus was more divergent than the C-terminus and GhMCTPs’ functional divergence might be mainly contributed by the N-terminus. Furthermore, yeast two-hybrid assay indicated that the N-terminus was responsible to interact with target proteins. Phylogenetic analysis classified multiple N-terminal C2 domains into four subclades, suggesting that these C2 domains performed different molecular functions in mediating the transport of target proteins. Conclusions: Our systematic characterization of MCTPs in G. hirsutum will provide helpful information to further research GhMCTPs’ molecular roles in mediating other regulators’ transport to coordinate growth and development of various cotton tissues.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile2TableS1.xlsx
- Additionalfile2TableS1.xlsx
- Additionalfile5TableS2.xlsx
- Additionalfile5TableS2.xlsx
- Additionalfile9TableS5.xlsx
- Additionalfile9TableS5.xlsx
- Table1.xlsx
- Table1.xlsx
- Additionalfile4FigureS3.tif
- Additionalfile4FigureS3.tif
- Additionalfile1FigureS1.tif
- Additionalfile1FigureS1.tif
- Additionalfile3FigureS2.tif
- Additionalfile3FigureS2.tif
- Additionalfile7FigureS4.tif
- Additionalfile7FigureS4.tif
- Additionalfile6TableS3.xlsx
- Additionalfile6TableS3.xlsx
- Additionalfile8TableS4.xlsx
- Additionalfile8TableS4.xlsx
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