Yield and survival rate of ‘Gigante’ cactus pear under regulated decit irrigation using wastewater

This study aimed to evaluate the yield and survival rate of 'Gigante' cactus pear (Opuntia fícus indica) cultivated with regulated decit irrigation (RDI) using wastewater under semiarid soil and climatic conditions. The experiment was carried out between October 2015 and August 2017 at Instituto Federal Baiano, campus Guanambi, Brazil. The treatments were as follows: no fertilization and no irrigation (T1); no fertilization and RDI with wastewater (0.6 L plant -1 week -1 ) (T2); no fertilization and RDI with wastewater (1.2 L plant -1 week -1 , applied once a week) (T3); no fertilization and RDI with wastewater (1.2 L plant -1 week -1 , divided into two applications per week) (T4) with organic fertilization (60 Mg ha -1 of bovine manure) and RDI with common water (1.2 L plant -1 week -1 ) (T5) and with organic fertilization (60 Mg ha -1 of bovine manure) and no irrigation (T6). The treatments were arranged in a randomized complete block design with ve replicates. Based on the results, we concluded that (i) regulated decit irrigation using wastewater increased the productivity of 'Gigante' cactus pear when compared to the rainfed crop and (ii) the application of 0.6 L plant -1 week -1 was sucient to increase the survival rate of 'Gigante' cactus pear under prolonged drought conditions.


Introduction
Semiarid regions are characterized by scarce and irregular rainfall, either in spatial or temporal distribution, sometimes concentrating large volumes in a short period of time, followed by long periods of drought. This variability in rainfall pattern creates di culties in the production and availability of forage for livestock during the dry season (Correia et al., 2010).
In this scenario, crops and livestock are heavily affected by prolonged periods of drought, even when the annual rainfall is close to or above the annual average, due to its irregular distribution over the year (Duarte et al., 2018). Adverse conditions limit crop production, thus making raising livestock the main source of income in this region.
Water scarcity in agriculture requires practices for the rational use and reuse of water; innovations in agricultural systems need research aimed at nding alternative sources of input, thereby making agriculture feasible and boosting its development (Lemos et al., 2018).
Therefore, cactus pear emerges as an alternative because it is a xerophytic plant with physiological characteristics that allow a better use of water. This crop is well adapted to semi-arid conditions and is widely used to feed herds in the Brazilian northeastern region (Cordova-Torres et al, 2017).
One of the factors limiting productivity is associated with scarcity or lack of rainfall because of either its small volume or poor distribution. This factor also decreases the survival rate of recently planted cactus pear. An alternative to change this scenario would be the use of irrigation (Pereira et al., 2015).
Regulated de cit irrigation (RDI) works on the premise that crops cope with a reduced water supply by reducing transpiration (stomata regulation or reducing leaf surface area through reducing leaf growth) (Wilkinson and Hartung, 2009) or closing the stomata during the day and opening them at night for CO 2 xation, such as cactus pear. In this sense, a controlled water de cit during particular periods may bene t water productivity (WP) by increasing irrigation water savings, minimizing or eliminating negative impacts on yield and crop revenue and even improving harvest quality.
Considering that good quality water is scarce in semi-arid regions and should be preferably used for domestic and human supply (Brazil, 2005), it is possible to use wastewater to increase yields and solve social and environmental problems of rural families and communities.
Furthermore, wastewater is an alternative source of nutrients, such as nitrogen, potassium, phosphorus, calcium, and magnesium (Medeiros et al., 2011); thus, wastewater can replace, in whole or in part, the need for chemical or organic fertilizers.
This study aimed to evaluate the yield and survival rate of the 'Gigante' cactus pear (Opuntia fícus indica) cultivated with regulated de cit irrigation using wastewater in the semi-arid region of Bahia state, Brazil.

Material And Methods
The experiment was conducted at the Federal Institute of Education, Science, and Technology Baiano, campus Guanambi, Bahia state, Brazil (14º 13' 30"S and 42º 46' 53"W). Semiarid is the predominant climate; the mean annual rainfall is 663.69 mm, the mean annual evapotranspiration (ET) rate is 1961.6 mm, and the mean temperature is 26 °C.
The soil was classi ed as an atypical medium-textured dystrophic yellow red Latosol (Embrapa, 1999). Physical and chemical characterizations of the soil were performed before implementing the experiment using soil samples The crop used in the experiment was cactus pear (Opuntia cus indica), cultivar Gigante. The experiment was carried out from October 2015 to August 2017. During this period, the main climatic parameters (wind speed, air temperature, relative humidity, net radiation and precipitation) were monitored using an automatic weather station installed near the experimental area.
The yield and survival rate of 'Gigante' cactus pear under RDI using wastewater were evaluated. The experiment was designed in randomized blocks with six treatments and ve replicates. The treatments were as follows: T1: no fertilization and no irrigation; T2: no fertilization and RDI with wastewater (0.6 L plant -1 week -1 ); T3: no fertilization and RDI with wastewater (1.2 L plant -1 week -1 , applied once a week); T4: no fertilization and RDI with wastewater (1.2 L plant -1 week -1 , divided into two applications of 0.6 L plant -1 week -1 ); T5: with organic fertilization (60 Mg ha -1 of bovine manure, applied before planting) and RDI with common water (1.2 L plant -1 week -1 ); and T6: with organic fertilization (60 Mg ha -1 of bovine manure applied before planting) and no irrigation.
The experimental plot consisted of three 6-m-long rows of plants spaced 1 m apart (30 plants per row, spaced 0.2 m apart), with a 30 m 2 area (6 m x 5 m -including a 3-m-wide path), with a stand of 30,000 plants ha -1 . In the blocks, the treatments succeeded each other without additional spacing, so only the plants within the 4-m-long central row of each plot (20 plants per row, 60 plants in total) were evaluated. The remaining plants were borders. Thus, each block was 36 m long and 2 m wide, spaced apart by a 3-m-wide path. On the outer sides, there was also a 3-m-wide path surrounding the experimental area.
The area was subsoiled, plowed, harrowed and then furrowed at a distance of one meter between furrows. Bovine manure was applied only in the planting furrow of the plots of the T5 and T6 treatments (60 Mg ha -1 ). Mature cladodes with accumulation of reserves were selected in another cactus pear plantation of the campus; after harvest, they remained in the shade for 15 days to cure. After curing, the cladodes were planted with the longest portion buried approximately 50% in the soil for better xation at a distance of one meter between planting rows. The cladodes were spaced 20 cm apart. Weeds were mechanically controlled during the experiment. Planting was completed at the end of October 2015.
The wastewater was collected from a stabilization pond that receives domestic sewage collected from campus buildings. It was stored for 24 hours in a water tank (5000 L) before using it for irrigation so that the larger particles could settle on the bottom of the tank, reducing clogging problems.
The common water was collected in a tubular well installed on campus and stored in a water tank (500 L). Both irrigations, with common and wastewater, were performed using a drip irrigation system consisting of a submersible pump with a power of 450 W and an output diameter of 1"; a 200 mesh disk lter with an output diameter equal to 1"; a A wastewater sample was collected every four months, from April 2016 to August 2017, totaling ve samples. The wastewater samples were integrated into an average sample and subjected to laboratory analysis to determine nutrient levels, pH, and electrical conductivity. The common water and bovine manure were also analyzed.
The pH readings of wastewater (WW) and common water (CW) were 7.1 and 6.8, respectively, with electrical conductivities of 1.0 and 2.9 dS m -1 , respectively. The average levels of macro-and micronutrients present in WW, CW and bovine manure (BM) are presented in Table 1. At each wastewater evaluation, the irrigation system was evaluated as well. Mean weekly water depth (Dm) and the uniformity of water distribution (DU) were evaluated at each irrigated treatment. The calculation of Dm took into account the mean ow rates (Fm) multiplied by the irrigation time of each treatment and then divided by the wet area of the emitter.
The total volume of wastewater applied to each treatment was obtained by multiplying Fm by weekly irrigation time and number of weeks. This volume multiplied by the wastewater nutrient contents resulted in the contribution of nutrients for the plants in T2, T3, and T4.
Precipitation and reference evapotranspiration (ETo) data, obtained from an automatic weather station installed on campus, and Dm were used to perform the crop water balance (CWB), according to the method proposed by Thornthwaite and Mather (1955). CWB was used for the whole experimental period to determine the water de cit of the crop in all treatments. The CWB was set up using Dm applied on all irrigated days to obtain the total irrigation (I) in the irrigated treatments. For this, the crop coe cient (Kc) was considered to be 0. The pH, CL and CW were determined using a measuring tape. NOC was determined by counting all cladodes on the mother plant. To measure the height of the plant, the distance from the ground to the tip of the highest cladode was considered.
The length and width of the cladodes were determined using the longest straight line across the cladode. All cladodes of evaluated plants were measured. The cladode area was determined using the methodology described by Pinto et al. To determine dry matter yield (DMY, in Mg ha -1 ), thirty cladode samples per treatment were collected using a hole saw (5.00 cm in diameter by 4.00 cm deep) coupled to a battery powered drill. The total mass of thirty samples taken in each treatment ranged from 1,500 to 2,000 g. The samples were dried in a forced-air oven at 60 ºC for 72 h. Dry matter content, in percentage (DM%), was determined as described by Silva & Queiroz (2009 (Ferreira, 2011). Statistical analyses were performed using the statistical program "Sisvar" (Ferreira, 2014).

Water Distribution Uniformity
The mean ow rates of drippers, distribution uniformity, coe cient of variation of ow, and mean weekly water depth applied to each irrigated treatment after ve evaluations of the irrigation system are shown in Table 2. with bovine manure (60 Mg ha -1 ) and RDI with common water (1.2 L plant -1 week -1 ).

Crop Water Balance (CWB)
Monthly precipitation and potential crop evapotranspiration (ETpc) during the experiment are depicted in Figure 1.

Plant Height (PH)
Representative models of the evolution of mean plant height over time (280 to 640 DAP) and their respective regression equations are shown in Figure 2.
The best ts were obtained with third-degree polynomial equations (P <0.05). The tted models are justi ed by the occurrence of two dry periods, with pronounced water de cit and decreased plant growth, a rainy period in between, with increased plant growth. There was only a signi cant difference for NOC as a function of time in treatments T5 and T6.

Length and Width of Cladodes
Representative models of the evolution of the mean length and width of cladodes over time (280 to 640 DAP) are shown in Figures 4 and 5, respectively.
Observing the growth models tted to cladode length and width as a function of DAP (Figures 4 and 5, respectively), it appears that the increase in cladode length and width occurred slowly and proportionally, speeding up in the rainy season. Hence, again, the best ts were obtained with third degree polynomial equations (P <0.05).
Both the mean length and mean width of cladodes in T6 were lower than in all other treatments, which did not differ statistically throughout the experiment (280 to 640 DAP).
3.6 Cladode Area Index (CAI) Figure 6 presents the representative models of the evolution of the mean cladode area index (280 to 640 DAP) and their respective regression equations. Again, the best ts were obtained with third degree polynomial equations (P<0.05).
The models were tted for the same reasons as the aforementioned traits.
The models tted to the cladode area index as a function of DAP ( Figure 6) have a behavior akin to that of cladode length and width. It appears that the growth of the cladode area index (CAI) occurred slowly and proportionally, with an acceleration in the rainy season; hence, the best ts were obtained with third degree polynomial equations (P <0.05).

Number of Dead Plants (NDP)
Means of the number of dead 'Gigante' cactus pear plants are shown in Table 4. Observing the analysis of variance, there was a signi cant difference (P <0.05) for the treatments and periods and their interaction.

Green and Dry Matter Yield
Mean values of green matter yield (GMY), dry matter content (DM%) and dry matter yield (DMY) of 'Gigante' cactus pear plants cultivated without irrigation, irrigation with wastewater application, and irrigation with common water are presented in Table 5.  Table 5 shows that the GMY in T5 (with organic fertilization and RDI with common water) was higher than that in the other treatments (P <0.05). In T2, T3 and T4 (no fertilization and RDI with wastewater), means were statistically equal to one another (P> 0.05) and were higher than those of treatments T1 and T6 (no irrigation), which also did not differ from each other (P> 0.05).
The DMY in T1 (Table 5) was higher than in other treatments (P <0.1); treatments T1 and T6 did not differ from each other (P> 0.1) and were inferior to treatments T2, T3 and T4, which did not differ from each other either (P> 0.1). Table 5 shows that treatment T5, which received organic fertilization with 60 Mg ha -1 of bovine manure and was irrigated with common water, had the highest yields (GMY and DMY). This treatment was followed by treatments T2, T3, and T4, all without organic fertilization and irrigated with wastewater. These were superior to treatments T1 and T6, which were non-irrigated, with and without organic fertilization, whose yields were statistically equal.
Dry matter contents (Table 5) in the irrigated treatments (T2, T3, T4, and T5), which did not differ from each other (P> 0.05), were lower than in the treatments without irrigation (T1 and T6), which did not differ from each other either (P> 0.05).

Water Distribution Uniformity
The uniformity of water distribution, with DU ranging from 93 to 95%, can be considered excellent in all treatments, according to the evaluation criterion proposed by Mantovani (2001)  The determination of DU is important because it allows a more rigorous evaluation of the irrigation system and the adoption of measures aimed at maintaining high uniformity of water distribution, reducing the negative impact that lower irrigation levels may exert on plants. Table 3 shows that even for the crop with a low water demand (Kc=0.5), in the non-irrigated treatments (T1 and T6), the water de cit was equal to 73% [(1 -ETc/ETpc).100]. This means that the crop has failed to transpire a potential amount that is almost three times greater than what it had actually transpired. If we take into account a production function relating real yield and potential yield (1 -Yr/Yp) proportional to transpiration, the crop lost approximately three-quarters of its productive potential.

Crop Water Balance (CWB)
On the other hand, in the treatment with organic fertilization and water supplementation with common water (1.2 L week -1 plant -1 ) (T5), the water de cit was 35%, that is, the crop had not transpired just over a third of its potential evapotranspiration.
Evapotranspiration is directly linked to plant production, since water de cit has a direct effect on crop production. The water lost through evapotranspiration is responsible for various processes within plant cells, as well as being responsible for transporting nutrients available in the soil.

Plant Height (PH)
The rst two assessments (280 and 365 DAP) were made in the middle and at the end of the rst dry period; the third assessment (490 DAP) was made in the middle of the second rainy season; and the last two assessments (580 and 640 DAP) were made in the middle and at the end of the second dry period. Between the second (365 DAP) and third (490 DAP) assessments, most of the annual precipitation had already occurred, as shown in Figure 1 (408 mm).
The rainy period occurred in the intermediate phase of the research, creating conditions for a faster growth rate, which can be veri ed in all treatments. During the rainy season (between the second and third assessments), the mean plant height in all treatments increased considerably, in contrast to the two dry periods, during which plant height increased slowly. The tted third-degree models clearly show this behavior. The increase in water availability during the rainy season shows that the plant has satisfactory results under more favorable conditions, resulting in growth.

Number of cladodes (NOC)
Although there was a signi cant difference only for NOC over time in treatments T5 and T6, it is clear that there was an increasing trend of NOC over time in all treatments. Ramos et al. (2015) similarly found a linear increase in the total number of cladodes over time because, according to them, as the plant grows, there is an increase in the number of cladodes. According to Queiroz et al. (2015), the cactus pear responds more quickly to the emission of rst-and second-order cladodes when irrigated, showing that e cient water use by the plant is re ected in increased growth and development.

Length and Width of the Cladodes
Considering that there was no application of water in treatments T1 and T6, it was supposed that the average length and width of the cladodes in these treatments were lower than in the other treatments, which received irrigation.
Observing the average number of cladodes (Figure 3), we observed that in treatments T5 and T6, both fertilized means were higher than in the other treatments. Higher cladode sprouting negatively affected the mean length and width of cladodes in the T6 treatment when compared to that in T1, both of which were not irrigated. In T5, even with a higher number of cladodes, sprouting had no interfere with the mean cladode length and width, probably because the plants of this treatment were both irrigated and organically fertilized.
According to Lemos et al. (2018), the increase in cladode length always occurs in the rst months. Although the plant is under favorable conditions during its development, it does not in uence cladode length; the author also reports that the average length of cladodes is directly related to the availability of water and nutrients and the absorption of light energy used by the plant for photosynthesis, which is affected by spacing and planting density.
Azevedo Junior (2017), studying wastewater on cactus pear performance, observed that cladode length and width increased linearly, showing a direct relationship between width and length cladode with respect to growth rate. The cactus pear has a similar growth of cladodes, with longitudinal and perpendicular elongation of cladodes and cladode sprouting tending to grow slower over time.

Cladode Area Index (CAI)
The results were as expected since the CAI response rate is dependent on morphological characteristics such as cladode number, cladode length and width. The CAI is directly linked to the favorable conditions that contribute to the development of the plant.
From 365 DAP (Figure 6), the T5 plants showed higher CAI than the plants of the other treatments. In addition, the T6 treatment plants had higher CAI than the other treatment plants from 580 DAP.
According to Fonseca et al. (2019), an important physiological characteristic is the cladode area index, since the higher the CAI is, the larger the area for the absorption of photosynthetically active radiation and, consequently, the greater the crop yield. Among the factors that affect the CAI, the nutritional status of the plant stands out. Donato et al. (2014) pointed out that the CAI is a factor that determines the active photosynthetic area of the plant since it indicates the plant's ability to intercept sunlight to e ciently transform it into dry matter production. Padilha Junior (2016), in studies with planting density and fertilization, reported that the best CAIs resulted from fertilization, with rates above 30 Mg ha -1 ; CAI was not in uenced by planting density.
However, the higher CAI does not always imply higher productivity, since in the present work, in the calculation of the CAI, the area occupied by the plant in the soil was calculated considering the planting stand (30,000 plants ha -1 ).
However, throughout the experiment, there was different plant mortality across treatments, which in uenced overall productivity.

Number of Dead Plants (NDP)
Analyzing Table 4 and Figure 7, it can be seen that the number of dead plants in non-irrigated treatments tends to increase linearly, while in irrigated treatments, this mortality remains almost constant. Thus, it is evident that irrigation was fundamental for plant survival in these treatments. Considering that 'Gigante' cactus pear is a perennial plant that, if well managed, can produce for over 50 years (Dubex Junior., 2017), it is expected that the crop will undergo many periods of prolonged drought throughout its life cycle, which could compromise the plant stand with increasing mortality in non-irrigated treatments. Thus, in addition to ensuring higher productivity, irrigation, even with controlled de cits, as was the case in this work, can guarantee productivity throughout the crop's useful life. Table 4 shows that at 640 DAP, the average plant mortality in the non-irrigated treatments T1 and T6 was 55 and 36 dead plants, respectively, in a population of 90 plants in each treatment plot, which represents, on average, 61% and 40% mortality rates, respectively . In the treatments irrigated with wastewater, T2, T3, and T4, the number of dead plants represented, on average, a mortality rate ranging from 4% to 6%; in treatment T5, irrigated with common water and fertilized, the mortality rate was, on average, 16%; this rate was much lower than that of non-irrigated treatments but higher than that in the treatments irrigated with wastewater.
Among the non-irrigated treatments, plant mortality at 640 DAP was 35% higher in the non-fertilized treatment. Thus, it can be inferred from these results that organic fertilization contributed to better water retention by the plant, which further contributed to the reduction in mortality rate. The cactus pear has a high resilience capacity and can respond quickly when subjected to favorable conditions.
For several months, the crop was subjected to a combination of drought and high potential crop evapotranspiration ( Figure 1), which caused the crop to lose its resilience, leading several plants to die.

Green and Dry Matter Yield
The results obtained for green and dry matter yield were corroborated by studies already published by other researchers. Lima et al. (2015) state that irrigation applied to smaller depths favors the transport of nutrient solution needed by the plant, making it a viable option for production, even in adverse conditions. The availability of these nutrients in large quantities, in the form of organic fertilization (cattle manure), favored the plants of treatment T5 to obtain the best yield. Table 5 shows that the treatments T2, T3 and T4, to which wastewater was applied, at different depths and application forms, showed satisfactory results, since the productivity was higher than in non-irrigated treatments (T1 and T6), with or without fertilization. The yield in treatments receiving wastewater (T2, T3 and T4) was lower than that in treatment T5, which received the same irrigation depth with common water as T2, T3 and T4 but received organic fertilization (60 Mg ha -1 ).
The dry matter content was higher in the non-irrigated treatments than in the irrigated treatments, possibly due to the intense water de cit naturally imposed on the plants of these treatments.
Observing the CAI data ( Figure 6) together with the yield data (Table 5), it can be seen that the higher CAI of the T6 treatment in relation to the wastewater treatments (T2, T3 and T4) did not translate to higher productivity due to the high plant mortality in the T6 treatment, which did not occur in the treatments irrigated with wastewater. This con rms the importance of irrigation with wastewater, even with de cits, in ensuring productivity throughout the useful life of the crop.

Conclusions
The application of only 0.6 liters of wastewater per linear meter of cultivation once a week is su cient to increase the survival rate of 'Gigante' cactus pear (Opuntia cus indica) under prolonged drought conditions.