Background: To investigate the role and difference of autologous stromal vascular fraction（SVF）cells and allogeneic adipose-derived stem cells(ADSCs) in full-thickness skin graft.
Methods: SVF cells and ADSCs were prepared from the inguinal fat pad of the rats, and full-thickness skin on the back of the rats were transplanted in situ. then, SVF, ADSCs and PBS were injected under the graft. Gross survival, H-E staining, Masson staining, CD31 and VEGF immunofluorescence were observed on 3d, 7d and 14d respectively.
Results: the survival rate of SVF group was the best, followed by ADSCs group (p<0.05). the H-E and Masson staining structures of the SVF group were better than those of the other two groups under the microscope. CD31 and VEGF expression in SVF group at 7 days and 14 days after surgery was more obvious than that in ADSCs group and PBS group.
Conclusion: Subcutaneous injection of autologous SVF or allogeneic ADSCs cells can improve the survival rate of full-thickness skin grafts. In addition, the effect of autologous SVF cells is better than that of allogeneic ADSCs.