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Research article
Dwi Aris Agung Nugrahaningsih
Faculty of Medicine, Public Health, and Nursing,Universitas Gadjah Mada
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1616-3053
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background : Leakage following colorectal anastomosis surgery causes various complications associated with high morbidity and mortality, especially in pediatric patients. It might be caused by the use of non-steroidal anti-inflammatory drugs (NSAIDs) as postoperative analgesics. This study aimed to compare the effect of metamizole and paracetamol on colonic anastomosis and fibroblast activities, including proliferation, migration, and collagen synthesis, in Wistar rats. Methods: Rats were divided into control, paracetamol and metamizole groups. The colonic anastomosis was evaluated by determining the integrity of the muscle layers, the formation of granulation tissue, and mucosal anastomosis. Fibroblast activities were analyzed by measuring the proliferation, migration, and collagen synthesis. Results: Metamizole caused more damage to muscle layer integrity, more inhibition of granulation tissue formation in the anastomosis area and lower mucosal anastomosis compared with paracetamol and control groups. Metamizole had a higher cytotoxic effect than paracetamol, which suppressed the proliferation and migration of fibroblasts. Furthermore, both drugs did not affect the synthesis of collagen. Conclusion: Metamizole shows worse effects on the integrity of muscle layers, inhibition of granulation tissue formation, mucosal anastomosis, fibroblast proliferation, and migration, but not collagen synthesis, than paracetamol in Wistar rat intestines following colonic anastomosis. These findings might indicate that paracetamol is safer than metamizole as analgesic following colonic anastomosis. Key Words: colonic anastomosis; fibroblast activities; metamizole; paracetamol; Wistar rat
Keywords
colonic anastomosis; fibroblast activities; metamizole; paracetamol; Wistar rat
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CURRENT STATUS: Published
View the published article at BMC Pharmacology and Toxicology.
No community comments so far
Submission checks complete
On 31 Dec, 2019
Editorial decision: Accept
On 31 Dec, 2019
Version 2
Posted 27 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 27 Dec, 2019
Review #1 received
Received 24 Dec, 2019
Reviewers invited
Invitations sent on 23 Dec, 2019
Reviewer #1 agreed
On 23 Dec, 2019
Reviewer #2 agreed
On 23 Dec, 2019
Review #2 received
Received 23 Dec, 2019
Editor assigned
On 22 Dec, 2019
Submission checks complete
On 21 Dec, 2019
Editor invited
On 21 Dec, 2019
No comments provided
Editorial decision: Major revision
On 21 Nov, 2019
Review #1 received
Received 31 Oct, 2019
Review #2 received
Received 31 Oct, 2019
Reviewer #2 agreed
On 21 Oct, 2019
Reviewer #1 agreed
On 11 Oct, 2019
Reviewers invited
Invitations sent on 10 Oct, 2019
Submission checks complete
On 30 Sep, 2019
Editor assigned
On 26 Sep, 2019
Editor invited
On 25 Sep, 2019
First submitted
On 23 Sep, 2019
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A new preprint design is coming!
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See the published version of this preprint at BMC Pharmacology and Toxicology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Dwi Aris Agung Nugrahaningsih
Faculty of Medicine, Public Health, and Nursing,Universitas Gadjah Mada
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1616-3053
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Pharmacology and Toxicology.
No community comments so far
Submission checks complete
On 31 Dec, 2019
Editorial decision: Accept
On 31 Dec, 2019
Version 2
Posted 27 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 27 Dec, 2019
Review #1 received
Received 24 Dec, 2019
Reviewers invited
Invitations sent on 23 Dec, 2019
Reviewer #1 agreed
On 23 Dec, 2019
Reviewer #2 agreed
On 23 Dec, 2019
Review #2 received
Received 23 Dec, 2019
Editor assigned
On 22 Dec, 2019
Submission checks complete
On 21 Dec, 2019
Editor invited
On 21 Dec, 2019
No comments provided
Editorial decision: Major revision
On 21 Nov, 2019
Review #1 received
Received 31 Oct, 2019
Review #2 received
Received 31 Oct, 2019
Reviewer #2 agreed
On 21 Oct, 2019
Reviewer #1 agreed
On 11 Oct, 2019
Reviewers invited
Invitations sent on 10 Oct, 2019
Submission checks complete
On 30 Sep, 2019
Editor assigned
On 26 Sep, 2019
Editor invited
On 25 Sep, 2019
First submitted
On 23 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Pharmacology and Toxicology.
Background : Leakage following colorectal anastomosis surgery causes various complications associated with high morbidity and mortality, especially in pediatric patients. It might be caused by the use of non-steroidal anti-inflammatory drugs (NSAIDs) as postoperative analgesics. This study aimed to compare the effect of metamizole and paracetamol on colonic anastomosis and fibroblast activities, including proliferation, migration, and collagen synthesis, in Wistar rats. Methods: Rats were divided into control, paracetamol and metamizole groups. The colonic anastomosis was evaluated by determining the integrity of the muscle layers, the formation of granulation tissue, and mucosal anastomosis. Fibroblast activities were analyzed by measuring the proliferation, migration, and collagen synthesis. Results: Metamizole caused more damage to muscle layer integrity, more inhibition of granulation tissue formation in the anastomosis area and lower mucosal anastomosis compared with paracetamol and control groups. Metamizole had a higher cytotoxic effect than paracetamol, which suppressed the proliferation and migration of fibroblasts. Furthermore, both drugs did not affect the synthesis of collagen. Conclusion: Metamizole shows worse effects on the integrity of muscle layers, inhibition of granulation tissue formation, mucosal anastomosis, fibroblast proliferation, and migration, but not collagen synthesis, than paracetamol in Wistar rat intestines following colonic anastomosis. These findings might indicate that paracetamol is safer than metamizole as analgesic following colonic anastomosis. Key Words: colonic anastomosis; fibroblast activities; metamizole; paracetamol; Wistar rat
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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