Clinicopathological parameters (Table 1).
The clinicopathological parameters of patients are summarized in Table 1. A total of 408 cases were included in the analysis,there were 272 males(66.7%) and 136 females(33.3%)at the age of 35-83 years (58.30±10.63 years). The tumor diameter was 4.55±1.87cm.Based on the 8th TNM (UICC/AJCC), 48 were at stage pT1(11.8%), 48 at stage pT2(11.8%), and 280 at pT3(68.6%), 32 at pT4(7.8%), respectively.As for degree of differentiation, 136 were differentiated(33.3%) and 272 were undifferentiated(66.7%). There were 244 (40.2%) vascular invasions and 164 (59.8%) non-invasions. There were 244 (40.2%) nerve invasion and 164 (59.8%) nerve non-invasion.Cancer nodules were 36 (8.8%) and absent 372 (91.2%).LNS ranged from 12 to 73 (31.88±10.80). PLNS ranged from 0 to 32 (7.55±8.10). rN was 0-0.911 (0.230±0.231). rN1 was 0-0.840 (0.245±0.237). rNS1 was 0-1.000 (0.178±0.266).
The influence of rN1 and rN2 on the prognosis for patients (figure1.2):
The best cut-off values of rN1 and rN2 ,were calculated by survival analysis and kaplan-meier and log-rank,were 0.140 and 0.100, respectively. There was significant difference in 5-year survival ratio between rN1 >0.140 and rN1 <0.140 (p<0.05). As rN1 gradually increased, the 5-year survival ratio gradually declined.When rN1 exceeded 0.140, 5-year survival ratio significantly decreased, which was statistical significance. The same as above,it was significant difference for rN2 >0.100 and rN2 <0.100 (p<0.05). As rN2 gradually increased, 5-year survival ratio gradually decreased. When rN2 exceeded 0.100, 5-year survival ratio significantly decreased, which was statistically significant.
Univariate and multivariate analysis (Table 2, Table 3) : Univariate and multivariate analysis of prognostic factors were analyzed by Cox proportional hazards model,gender (B= -0.170 P=0.551>0.05), age (B= 0.015 P=0.255>0.05), nerves invaded (B=0.507 P=0.063>0.05),LNS(B=0.017 P=0.175>0.05),LNS1 (B=0.021 P=0.139>0.05), LNS2(B=0.007 P=0.858>0.05) were non-prognostic factors,tumor size(B=0.501 P=0.001<0.05), depth of invasion(B=0.989 P=0.001<0.05),blood vessels invaded (B=0.619 P=0.023<0.05),carcinoma nodules(B= 0.825 P=0.042<0.05),degree of differentiation(B=0.484 P=0.038<0.05),rN(B=2.424 P=0.001<0.05),PLNS1(B=0.098P=0.001<0.05),rN1(B=3.244 p=0.001<0.05),PLNS2(B=0.126 p=0.001<0.05), rN2(B=3.664 p=0.001<0.05), pTNM (B=3.861 p=0.001<0.05) were prognostic factors. Among factors, the prognostic factors were pTNM > rN2 > rN1 >rN, it is suggested that rN1 and rN2 are effective and reliable prognostic index.