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Research article
JIPING LIU
Robert W. Holley Center, USDA-ARS
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7558-0025
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: In Arabidopsis, the aluminum (Al) exclusion mechanism is mainly facilitated by ALMT1-mediated malate exudation and MATE-mediated citrate releases from the root. Recently, we have demonstrated that coordinated functioning between the ALMT1-mediated exclusion mechanism, via exudation of malate from the root tip, and a NIP1;2-facilitated internal detoxification mechanism, via removal of Al from the root cell wall and subsequent root-to-shoot Al translocation, plays critical roles in achieving overall Al resistance. However, the genetic relationship between ALMT1 and NIP1;2 in these processes remained unclear.
Results: Through genetic and physiological analyses, we demonstrate that unlike ALMT1 and MATE, which function independently and additively, ALMT1 and NIP1;2 show an epistatic relationship in Al resistance in Arabidopsis. These results indicate that ALMT1 and NIP1;2 function in the same biochemical pathway, whereas ALMT1 and MATE in different biochemical pathways.
Conclusion: The establishment of the epistatic relationship and the coordinated functioning between the ALMT1 and NIP1;2-mediated exclusion and internal detoxification mechanisms are pivotal for achieving overall Al resistance in the non-accumulating Arabidopsis plant. We discuss and emphasize the indispensable roles of the root cell wall for the implementation of the Al exclusion mechanism and for the establishment of the epistatic relationship between the ALMT1-mediated exclusion mechanism and the NIP1;2-facilitated internal detoxification mechanism.
Keywords
ALMT1; aluminum resistance; epistasis; exclusion mechanism; internal detoxification mechanism; NIP1;2
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CURRENT STATUS: Published
View the published article at BMC Plant Biology.
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On 11 Mar, 2020
Editorial decision: Accept
On 11 Mar, 2020
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Editorial decision: Minor revision
On 09 Mar, 2020
Editor assigned
On 02 Mar, 2020
Submission checks complete
On 01 Mar, 2020
Editor invited
On 01 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 01 Mar, 2020
Review #1 received
Received 25 Feb, 2020
Reviewers invited
Invitations sent on 06 Feb, 2020
Reviewer #1 agreed
On 06 Feb, 2020
Editor assigned
On 03 Feb, 2020
Submission checks complete
On 02 Feb, 2020
Editor invited
On 02 Feb, 2020
Version 1
Posted 08 Oct, 2019
No comments provided
Editorial decision: Major revision
On 05 Jan, 2020
Review #2 received
Received 23 Dec, 2019
Reviewer #3 agreed
On 09 Dec, 2019
Reviewer #2 agreed
On 29 Nov, 2019
Review #1 received
Received 20 Nov, 2019
Reviewer #1 agreed
On 31 Oct, 2019
Reviewers invited
Invitations sent on 22 Oct, 2019
Submission checks complete
On 01 Oct, 2019
Editor assigned
On 25 Sep, 2019
Editor invited
On 24 Sep, 2019
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On 23 Sep, 2019
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See the published version of this preprint at BMC Plant Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
JIPING LIU
Robert W. Holley Center, USDA-ARS
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7558-0025
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Plant Biology.
No community comments so far
Submission checks complete
On 11 Mar, 2020
Editorial decision: Accept
On 11 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 09 Mar, 2020
Editor assigned
On 02 Mar, 2020
Submission checks complete
On 01 Mar, 2020
Editor invited
On 01 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 01 Mar, 2020
Review #1 received
Received 25 Feb, 2020
Reviewers invited
Invitations sent on 06 Feb, 2020
Reviewer #1 agreed
On 06 Feb, 2020
Editor assigned
On 03 Feb, 2020
Submission checks complete
On 02 Feb, 2020
Editor invited
On 02 Feb, 2020
Version 1
Posted 08 Oct, 2019
No comments provided
Editorial decision: Major revision
On 05 Jan, 2020
Review #2 received
Received 23 Dec, 2019
Reviewer #3 agreed
On 09 Dec, 2019
Reviewer #2 agreed
On 29 Nov, 2019
Review #1 received
Received 20 Nov, 2019
Reviewer #1 agreed
On 31 Oct, 2019
Reviewers invited
Invitations sent on 22 Oct, 2019
Submission checks complete
On 01 Oct, 2019
Editor assigned
On 25 Sep, 2019
Editor invited
On 24 Sep, 2019
First submitted
On 23 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Plant Biology.
Background: In Arabidopsis, the aluminum (Al) exclusion mechanism is mainly facilitated by ALMT1-mediated malate exudation and MATE-mediated citrate releases from the root. Recently, we have demonstrated that coordinated functioning between the ALMT1-mediated exclusion mechanism, via exudation of malate from the root tip, and a NIP1;2-facilitated internal detoxification mechanism, via removal of Al from the root cell wall and subsequent root-to-shoot Al translocation, plays critical roles in achieving overall Al resistance. However, the genetic relationship between ALMT1 and NIP1;2 in these processes remained unclear.
Results: Through genetic and physiological analyses, we demonstrate that unlike ALMT1 and MATE, which function independently and additively, ALMT1 and NIP1;2 show an epistatic relationship in Al resistance in Arabidopsis. These results indicate that ALMT1 and NIP1;2 function in the same biochemical pathway, whereas ALMT1 and MATE in different biochemical pathways.
Conclusion: The establishment of the epistatic relationship and the coordinated functioning between the ALMT1 and NIP1;2-mediated exclusion and internal detoxification mechanisms are pivotal for achieving overall Al resistance in the non-accumulating Arabidopsis plant. We discuss and emphasize the indispensable roles of the root cell wall for the implementation of the Al exclusion mechanism and for the establishment of the epistatic relationship between the ALMT1-mediated exclusion mechanism and the NIP1;2-facilitated internal detoxification mechanism.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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