Cytological evidence of BSD2 functioning in both chloroplast division and dimorphic chloroplast formation in maize leave

doi:10.21203/rs.2.15741/v2

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Cytological evidence of BSD2 functioning in both chloroplast division and dimorphic chloroplast formation in maize leave

https://doi.org/10.21203/rs.2.15741/v2

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published 09 Jan, 2020

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Background: Maize bsd2 (bundle sheath defective2) is a classical C4 mutant with defective C4 photosynthesis, accompanied with reduced accumulation of Rubisco (ribulose bisphosphate carboxylase oxygenase) and aberrant mature chloroplast morphology in the bundle sheath (BS) cells. However, as a hypothetical chloroplast chaperone, the effects of BSD2 on C4 chloroplast development have not been fully examined yet, which precludes a full appreciation of BSD2 function in C4 photosynthesis. The aims of our study are to find out the role of BSD2 in regulating chloroplasts development in maize leaves, and to add new insights into our understanding of C4 biology. Results: We found that at the chloroplast maturation stage, the thylakoid membranes of chloroplasts in the BS and mesophyll (M) cells became significantly looser, and the grana of chloroplasts in the M cells became thinner stacking in the bsd2 mutant when compared with the wild type plant. Moreover, at the early chloroplast development stage, the number of dividing chloroplasts and the chloroplast division rate are both reduced in the bsd2 mutant, compared with wild type. Quantitative reverse transcriptase-PCR analysis revealed that the expression of both thylakoid formation-related genes and chloroplast division-related genes is significantly reduced in the bsd2 mutants. Further, we showed that BSD2 interacts physically with the large submit of Rubisco (LS) in Bimolecular Fluorescence Complementation assay. Conclusions: Our combined results suggest that BSD2 plays an essential role in regulating the division and differentiation of the dimorphic BS and M chloroplasts, and that it acts at a post-transcriptional level to regulate LS stability or assembly of Rubisco.

Plant Physiology and Morphology

Plant Molecular Biology and Genetics

Maize

C4 photosynthesis

Kranz anatomy

BSD2

chloroplast division

Rubisco

On the earth, although only less than 5% of terrestrial plants can use C₄ photosynthesis, they give a quarter of the primary productivity [1]. C₄ plants have higher utilization efficiency of water and nitrogen than C₃ plants, due to a series of biochemical and anatomical modifications occurred in C₄ plants [2, 3]. A characteristic feature of C₄ plants is formation of Kranz anatomy, whereby concentric wreaths of mesophyll (M) and bundle sheath (BS) cells surround closely spaced veins in the leaf (Kranz is German for wreath) [4]. The Kranz anatomy provides a structural framework for compartmentalization of photosynthetic enzymes and proteins in two morphologically and physiologically distinct C₄ cell types, the BS cell and M cell. Particularly, ribulose-1,5-bisphosphate carboxylase/ oxygenase (Rubisco), the enzyme that catalyzes the fixation of CO₂for photosynthesis, exists exclusively in BS chloroplasts [5, 6, 7]. It is believed that compartmentation of Rubisco away from atmospheric CO₂confers C₄ plants a photosynthetic advantage under high light/temperature growth conditions or arid lands [1, 8].

Considering the importance of C₄ photosynthesis, there is tremendous interest in understanding how the development of Kranz anatomy is regulated, which is essential for the genetic engineering of C₃ crop species (such as rice) with C₄ photosynthesis characteristics [9, 10, 11]. Maize (Zea Mays) is a typical NADP-ME-type (NADP-malic enzyme) C₄plants with a relatively simple Kranz structure characterized by a single BS layer with centrifugally distributed agranal chloroplasts. The simplicity and the well-developed genetic resources have made maize an attractive model for studying the biology of C₄ development [12]. The M chloroplasts in maize are starchless and possess numerous grana, whereas the BS chloroplasts accumulate starch grains and their thylakoids are largely unstacked [13, 14]. The initial carbon fixation happens in M chloroplasts, where phosphoenolpyruvate (PEP) is carboxylated by phosphoenolpyruvate carboxylase (PEPCase) to form oxaloacetate (OAA). OAA is metabolized into malate, and then malate diffuses into the BS cells where it is decarboxylated to provide increased concentration of CO₂ around Rubisco. Finally, PEP is regenerated by pyruvate orthophosphate dikinase (PPDK) in the mesophyll cells. This CO₂concentration mechanism inhibits the oxidative reaction of Rubisco and reduces the energy waste in the photorespiratory pathway, leading to a significant increase in photosynthetic yield [15, 16].

To identify the regulators of the Kranz anatomy development, mutagenized maize populations were screened for mutations that specifically disrupt the photosynthetic enzyme accumulation patterns in either bundle sheath or mesophyll cells [17]. The bundle sheath defective2 (bsd2) mutant is defective in C₄photosynthesis and it has abnormal BS chloroplasts and reduced accumulation of Rubisco [18]. Molecular cloning revealed that BSD2 encodes a chloroplast-targeted protein which shares homology with the DnaJ class of molecular chaperones [5]. Further studies showed that BSD2 is not involved in general photosynthetic complex assembly or protein import. In the bsd2 mutants, although the Rubisco proteins could not be detected, the chloroplast-encoded Rubisco large subunit transcript (rbcL) was abundant and associated with polysomes in both the M and BS cells. Therefore, it was suggested that BSD2 plays a direct role in the post-transcriptional control of rbcL transcripts accumulation and/or translation, and an indirect role in the maintenance of chloroplast structure in the BS cells. On the other hand, it was reported that chloroplast structure in M cells is not perturbed in the bsd2 mutant [5, 18]. However, the biochemical function of BSD2 and its regulatory mechanism in dimorphic chloroplast development have remained essentially unknown.

In this study, we showed that the BS chloroplasts have more grana, but their number decreased significantly in the bsd2 mutant, compared with the wild type. In addition, we found that M chloroplasts are also defective in the bsd2 mutant, characterized with thinner grana. Moreover, we found that at the proplastid stage, the number of dividing plastids is abnormal in the bsd2 mutants. Together, our results suggest BSD2 plays an important role in regulating both the division and formation of dimorphic chloroplasts in maize leaves, thus adding new insights into our understanding of C₄biology.

Mutation of BSD2 Disturbs chloroplast grana stacking and starch accumulation

To investigate the roles of BSD2 in the regulation of leaf development, we examined the leaf anatomy in twelve-day old bsd2 mutant and wild type plants (Fig. 1A). Light micrograph observation showed that the M and BS cells of the Kranz anatomy were well developed in leaves of both wild type and the bsd2 mutant (Fig. 1B). However, the distribution of the chloroplasts in BS cells of wild type and the bsd2 mutant differed significantly (Fig. 1B). In wild type, most of the BS chloroplasts were located close to the M cells, while BS chloroplasts were randomly distributed in the bsd2 mutant. On the other hand, M chloroplasts were randomly distributed along the cell wall and no significant difference was found between wild type and the bsd2 mutant (Fig. 1B).

We further investigated whether the bsd2 mutation affects the ultra-structure of the mature chloroplasts in the bsd2 mutant (Fig. 1C). Electron microscopy observation showed that the wild type BS chloroplasts were oval-shaped, with unstacked stromal lamellae and large starch granules of high electronic density (Fig. 1C1, 1C2). M chloroplasts in wild type were disk-shaped, but had well developed stacked thylakoids and accumulation of small starch granules (Fig. 1C1, 1C3). Compared with the wild type, BS chloroplasts in bsd2 mutant were swollen or damaged, and the thylakoid system was loosely arranged (Fig. 1C4, 1C5). Moreover, no starch granules were found in BS chloroplasts (Fig. 1C5). In addition, M chloroplasts of bsd2 were also swollen with the thinner grana, and the thylakoid system was more loosely arranged when compared with M chloroplasts in wild type (Fig. 1C4, 1C6).

Mutation of BSD2 Disturbs chloroplast development

To understand how the defects in BS and M chloroplasts occurred in the bsd2 mutant, we conducted a comparative developmental time course study of the chloroplast (or plastid) ultrastructure in wild type and the bsd2 mutant. Several key developmental stages of chloroplast were examined, including the proplastid stage (leaves still enclosed in the coleoptile, six-day old seedlings), chloroplast-grana differentiation stage (on the first day of leaves growing out of the coleoptile, seven-day old seedlings), the chloroplast dimorphism formation stage (on the second day after the leaves have grown out of the coleoptile, eight-day old seedlings), chloroplast maturation stage (on the third to fifth day after the leaves have grown out of the coleoptile, nine-to-eleven-day old seedlings). At the proplastid stage, proplastids varied in shape and were rich in starch granules and only had weak prothylakoids in both BS and M chloroplasts. No significant difference was found between wild type and the bsd2 mutant plants (Fig. 2A). At the chloroplast-grana differentiation stage, both BS and M chloroplasts developed stacked grana thylakoids and unstacked stromal thylakoids in both wild type and the bsd2 mutant (Fig. 2B). Still, no significant difference was found in chloroplasts between wild type and the bsd2 mutant plants at this stage. At the chloroplast dimorphism formation stage, on the second day after the leaves have grown out of the coleoptile, an increase in grana stacking (thylakoid membrane layers per granal stack) was found in the M chloroplasts in both wild type and the bsd2 mutant, while the BS chloroplast had a striking reduction in thylakoid stacking in both wild type and the bsd2 mutant. By this stage, chloroplast dimorphism was clearly formed (Fig. 2C). Notably, the thylakoid membranes of BS chloroplasts were more loose in the bsd2 mutant, when compared with the wild type (Fig. 2C). At the chloroplast maturation stage, the loosening of BS thylakoid membranes became more evident, accompanied by abnormal expansion of BS chloroplasts in the bsd2 mutant (Fig. 2D). At this stage, the BS chloroplasts in both wild type and the bsd2 mutant had developed unstacked thylakoid membranes. An increase in grana stacking was found in the M chloroplasts of both wild type and the bsd2 mutant. However, the thylakoid membranes were a little bit more loose, and the grana stacking was thinner and wider in the bsd2 mutant when compared with the wild type (Fig. 2D).These observations suggest that mutation in BSD2 disturbs development of both BS and M chloroplasts at the maturation stage, and the disturbance was much more severe in the BS chloroplasts.

We next performed quantitative RT-PCR to measure the transcript levels of ADL1A (encoding a dynamin-like protein) and VIPP1 (encoding the vesicle-inducing protein VIPP1), both of which encode structural components of thylakoid during chloroplast development [19, 20]. The transcript levels of both ADL1Aand VIPP1 were significantly reduced in bsd2 relative to the wild type, during the chloroplast-grana differentiation stage, dimorphism formation stage and the maturation stage (Fig. 2E), suggesting that BSD2 plays a role in promoting the expression of thylakoid formation genes.

bsd2 mutant has reduced chloroplast number in BS and M cells

To test whether chloroplast division is affected in the bsd2 mutant, we examined the ultrastructure of the chloroplasts (proplastid stage) in the first leaf of 6-day old wild type and bsd2 seedlings. Our results showed that wild type plants had more dividing chloroplasts than bsd2 (Fig. 3A). Next, we compared the chloroplast division rate (measured as the percentage of dumb-bell-shaped chloroplasts per randomly selected chloroplasts in leaf) in bsd2 and wild type at the proplastid stage. A decrease in chloroplast division rate (by 66.4% for BS chloroplasts and by 40.3% for M chloroplasts, n = ~100 chloroplasts from 20 cells of 8 samples) was found in the bsd2 mutant (Table 1), suggesting that BSD2 plays a role in promoting chloroplast division at the early developmental stage. We further compared the chloroplast number per cell at the maturation stage, and found a decrease of chloroplasts per cell (25.3% in BS chloroplasts and 18.2% in M chloroplasts, n = 20 cells) in the bsd2 mutant, compared with the wild type (Table 2). These observations indicate that BSD2 promotes chloroplast division in both M and BS cells.

We next performed quantitative RT-PCR to measure the transcript levels of genes encoding structural components of the chloroplast division apparatus [21]. The transcript levels of PDV1-1 (Plastid Dividing 1-1), PDV1-2 (Plastid Dividing 1-2), PDV2 (Plastid Dividing 2), FtsZ (Filamenting temperature-sensitive Z), ARC3 (Accumulation and Replication of Chloroplasts 3) and PDR1 (Plastid-Dividing Ring 1) were significantly reduced in bsd2 relative to the wild type plant (Fig. 3B), suggesting that BSD2 also plays a role in promoting the expression of chloroplast division-related genes.

BSD2 interacts with Rubisco large subunit (LS)

The Rubisco large subunit (LS) is encoded by the chloroplast RBCL gene. BSD2 has been hypothesized to act at a co- or post-translational step during LS synthesis [22, 23, 24]. Doron (2014) first indicated an association between BSD2 and the nascent LS peptide in Chlamydomonas based on biochemistry and molecular biology [25]. However, cytological evidence supporting this notion is still lacking. To verify whether BSD2 interacts with LS in vivo, we preform Bimolecular Fluorescence Complementation (BiFC) assay. Our results showed that indeed BSD2 interacted with LS in vivo (Fig. 4B). These observations add evidence to support the notion that BSD2 acts at a posttranscriptional level to regulate LS stability or assembly of Rubisco.

Cytological evidence for BSD2 functioning in regulating the development of both BS and M chloroplasts

BSD2 appears to be present only in green algae and plants, suggesting that it has evolved after the endosymbiotic event leading to the evolution of chloroplasts [26]. Previous studies showed that the mutation in BSD2 leads to abnormal BS chloroplasts (abnormal expansion and with loosen thylakoid membranes inside) and decreased chloroplast coverage in BS cells [18, 27], however, how BSD2 regulates chloroplast development has remained mysterious. In addition, it was reported that BSD2 does not affect M chloroplast development [5]. In this study, we found that the mutation in BSD2 not only affects BS chloroplast development, but also affects M chloroplast development. Our results showed that the BS chloroplasts have more grana, but their number decreased significantly in the bsd2 mutant, compared with the wild type. In addition, we found that M chloroplasts are also defective in the bsd2 mutant, characterized with thinner grana. This notion is further supported by the observed decrease in the expression of genes related to thylakoid formation. Furthermore, we found that chloroplast division is also affected in the bsd2 mutant as we observed fewer dividing proplastids in bsd2 during the early developmental stage (Fig. 3A) and that the division rate of chloroplasts is decreased in the bsd2 mutant (Table 1). This notion is also supported by the observed reduced expression of several genes related to chloroplast division (Fig. 3B). Thus, our combined results are consistent with the earlier report that chloroplast coverage is reduced in BS cells, likely due to a combinatory effort of decreased chloroplast division rate and abnormal chloroplast development in BS cells. Our findings that BSD2 also affects the division rate and development of M chloroplast uncover a previously unrecognized role of BSD2 in regulating kranz anatomy development. In this regard, it is particularly interesting to note that a recent study showed that cell type specific expression of BSD2 in BS or M cells driven by the RBCS or PEPC promoters all could complement Rubisco deficiency and seedling lethality conferred by the bsd2 mutation [27]. These new findings raise a possibility that BSD2 mRNA or its encoded product may be partitioned between the BS and M cell types. This will be an interesting topic for future studies.

It has been reported that the M chloroplasts of all C₄ species are randomly distributed along the cell wall, but BS chloroplasts are located either in a centripetal (close to the vascular tissue) or in a centrifugal (close to M cells) pattern, depending on the species [28]. Interestingly, we found that most of the BS chloroplasts are located close to the M cells in wild type, however, BS chloroplasts are randomly distributed in the bsd2 mutant, suggesting that BSD2 may also play a role in determining the distribution pattern of BS chloroplasts. However, the detailed mechanism remains to be explored.

Possible biochemical function of BSD2 in vivo

Previous studies reported that the correct folding and assembly of Rubisco requires multiple chaperone proteins, including BSD2, chaperonin 60-subunit a1 (Cpn60a), Cpn60b, 20-kDa chaperonin (Cpn20), Rubisco assembly factor 1 (RAF1), RAF2, and ribulose bisphosphate carboxylase factor X (RbcX) [29]. The Rubisco holoenzyme complex consists of 8 large subunits and 8 small subunits, and BSD2 was reported to play an essential role in the assembly process of eight LS subunits [26]. The most recent model suggested that, upon folding of the newly synthesized LS subunits by the Cpn60ab/Cpn20 chaperonin, LS assembly to LS dimers and higher oligomers is mediated by Raf1 and RbcX acting in cooperation or in parallel. Binding of BSD2 causes the displacement of these factors and stabilizes LS8 cores in a state competent for association with small subunits (SS). SS binding causes displacement of BSD2, forming the functional holoenzyme. Raf2 is essential for Rubisco biogenesis and may act downstream or upstream of chaperonin [26]. Our findings that BSD2 interacts physically with LS (in BiFC assays) add new evidence to support the notion that preassembly of LS/BSD2 intermediate complex is an early step during Rubisco assembly.

In short, our combined results suggest that BSD2 plays an indirect role in the maintenance of BS chloroplast structure by interacting physically with the large submit of Rubisco. Our data suggests that BSD2 is also required for the normal development and intactness of M chloroplasts. Moreover, based on our cytological observation and statistic analysis, we found that BSD2 most likely plays a role in regulating chloroplast division.

Plant material and growth conditions

Marty Sachs of the Maize Genetics Cooperation Stock Center and Prof. David B. Stern of Boyce Thompson Institute for Plant Research for provided the bsd2 mutant seeds.

The maize seeds collected from a heterozygous bsd2 mutant plant (about 25% of the progeny are bsd2 homozygous mutants) were planted in the Pindstrup Substrate nutritional soil (Denmark) in a growth chamber at 28°C with 16h-light (8000Lux)/8h-dark cycle.

Light Microscope and Electron Microscope Observation

Leaf sections (~2-3 mm²) from 6-12 days old wild type and bsd2 mutant plants were fixed in 4% (v/v) glutaraldehyde in 0.1 mol/L phosphate buffer solution (pH 7.2) for 4 h at 4 °C. Samples were washed three times with the same buffer, then post-fixed in 1% OsO₄ for 1hr at room temperature, and then transferred back into the phosphate buffer. After dehydration through an ethyl alcohol series, samples were embedded in Epon812 (SPI Supplies Division of Structure Probe Inc., West Chester, USA), polymerized for 24 h at 40 °C and then 24 h at 60 °C. Specimens were sectioned to a thickness of 1-2 μm with glass knives on a LEICA EM UC6 microtome. Sections were stained with Toluidine Blue O, examined and photographed using a Leica DMLB light microscope. Ultrathin sections (70-90 nm) were prepared using a diamond knife on a LEICA EM UC6 ultramicrotome, then stained with uranyl acetate and lead citrate. Sections were examined and photographed using a Phillips (Eindhoven, Netherlands) FEI-TECHNAI 12 TEM.

Expression analysis

Total RNA was isolated from maize leaves using TRIzol reagent (Invitrogen). First-strand cDNA was synthesized from ~1μg of total RNA with an oligo (dT) primer. Real-time PCR was performed on the BioRad CFX384 (USA). ACTIN was used as an internal standard for normalizing the cDNA concentrations. The sequences of the gene specific primers are listed in Table S2. Three biological replicates were used for each sample in the real-time PCR analysis.

Measurement of chloroplast number and statistic analysis

Leaf sections (~2-3 mm²) from the middle section of the third leaf in 12 days old wild type and bsd2 mutant plants were prepared and fixed in FAA (v/v; formaldehyde: glacial acetic acid: 70% ethanol = 1:1:18) for 24 h at room temperature. To estimate the number of mature chloroplasts per cell, the samples were separated into single-cell suspensions by treatment with a separation solution (v/v; 10% nitric acid: 10% chromic acid = 1:1) at 40 °C for approximately 4-6 h. Next, the samples were washed in water and placed on the microscope slide and stained with Toluidine Blue O. Then, the cover slip was pressed down sufficiently to separate the cells. The number of mature chloroplasts per cell was counted under the eyepiece of a Leica DMLB microscope (Germany).

The number of proplastids per cell was counted under Phillips FEI-TECHNAI 12 TEM (Eindhoven, Netherlands) using ultrathin sections selected from different batches of seedlings. Mean proplastid number was determined based on random selection of 20 cells from 5 sections.

For statistic analysis, each set of measurements represents the average ± SD of 20 cells observed in three different seedlings. Data were analyzed using the SPSS version 10.0 software. The significance level was set at p < 0.05.

BiFC assay

For BiFC assay, the full-length coding sequence of BSD2 was synthesized and cloned into the BiFC vector pVN to fuse with the N terminus of yellow fluorescent (YFP). The LS coding sequence and an 87 amino acids chloroplast targeting sequence of rice RBCS [30, 31] were synthesized and assembled into the BiFC vector pVC to fuse with the C terminus of YFP (GENEWIZ). Both of the vectors were co-transformed into rice protoplasts as described [32]. After incubation of the transformed protoplasts at 30 °C for 24 h, fluorescence images for YFP (green signal) were captured using a laser scanning confocal microscope (LEICA SP8 STED 3X). Auto-fluorescence (red signal) from chloroplast was also obtained.

ADL, a dynamin-like protein; ARC3, accumulation and replication of chloroplasts 3; BS, bundle sheath; Bsd2, Bundle sheath defective 2; FtsZ, filamenting temperature- sensitive Z; M, mesophyll; LS, Rubisco large subunit; Rubisco, ribulose-1,5- bisphosphate carboxylase/oxygenase; OAA, oxaloacetate; PDR1, plastid-dividing ring 1; PDV, plastid dividing; PEP, phosphoenolpyruvate; PPDK, pyruvate orthophosphate dikinase; TEM, transmission electron microscopy; VIPP1, the vesicle-inducing protein in plastid 1.

(1) Ethics approval and consent to participate

All participating authors have seen and approved the manuscript.

(2) Consent for publication

Not applicable.

(3) Availability of data and material

Not applicable.

(4) Competing interests

The authors declare that they have no competing interests.

(5) Funding

This work was financially supported by the National Natural Science Foundation of China under Grant No. 31470293. Authors declare that the funding agency was not involved in any experimental design, data analysis or manuscript writing.

(6) Authors' contributions

H. Wu. and H. L. designed the experiments. H. L., X. J., H. W., R. S., and M. B. conducted the experiments. H. L., M. B., H. Wu. analyzed the data. H. L., M. B., X. J., H. Wang. and H. Wu. wrote the paper.

(7) Acknowledgements

We thank Marty Sachs of the Maize Genetics Cooperation Stock Center and Prof. David B. Stern of Boyce Thompson Institute for Plant Research for providing the bsd2 mutant seeds.

1.Sage RF. The evolution of C4 photosynthesis. New Phytologist. 2004; 161: 341-370.

2.Zhu XG, Shan L, Wang Y, Quick WP. C₄ Rice-an Ideal Arena for Systems Biology Research. Journal of Integrative Plant Biology. 2010; 52: 762-770.

3.Ghannoum O, Evans JR, von Caemmerer S. Nitrogen and Water Use Efficiency of C4 Plants. Advances in Photosynthesis and Respiration. 2011; 32: 129-146.

4.Brown WV. Variations in anatomy, associations, and origins of Kranz tissue. American Journal of Botany. 1975; 62: 395-402.

5.Brutnell TP, Sawers RJH, Mant A, Langdale JA. BUNDLE SHEATH DEFECTIVE2, a novel protein required for post-translational regulation of the rbcL gene of maize. The Plant Cell. 1999; 11: 849-864.

6.Voznesenskaya EV, Franceschi VR, Kiirats O, Artyusheva EG, Freitag H, Edwards GE. Proof of C₄ photosynthesis without Kranz anatomy in Bienertiacycloptera (Chenopodiaceae). The Plant Journal. 2002; 31: 649-662.

7.Patel M, Berry JO. Rubisco gene expression in C₄ plants. Journal of Experimental Botany. 2008; 59: 1625-1634.

8.Hatch MD. C₄ photosynthesis: a unique blend of modiﬁed biochemistry, anatomy and ultrastructure. Biochemica Biophysica Acta. 1987; 895: 81–106.

9.Hibberd JM, Sheehy JE, Langdale JA. Using C4 photosynthesis to increase the yield of rice-rationale and feasibility. Current Opinion in Plant Biology. 2008; 11: 228-231.

10.Hibberd JM, Covshoff S. The regulation gene expression required for C₄ photosynthesis. Annual Review of Plant Biology. 2010; 61: 181-207.

11.von Caemmerer S, Quick WP, Furbank RT. The Development of C4 Rice: Current Progress and Future Challenges. Science. 2012; 336: 1671-1672.

12.Langdale JA, Lane B, Freeling M, Nelson T. Cell lineage analysis of maize bundle sheath and mesophyll cells. Developmental biology. 1989; 133: 128-139.

13.Lawton JR. Ultrastructure of chloroplast membranes in leaves of maize and ryegrass as revealed by selective staining methods. New Phytologist. 1988; 108: 277-283.

14.Brutnell TP, Langdale JA. Signals in leaf development. Advances in Botanical Research. 1998; 28: 161-195.

15.Sheen J. C4 gene expression. Annual Review of Plant Physiology and Plant Molecular Biology. 1999; 50: 187-217.

16.Majeran W, Cai Y, Sun Q, van Wijk KJ. Functional differentiation of bundle sheath and mesophyll maize chloroplasts determined by comparative proteomics. The Plant Cell. 2005; 17: 3111-3140.

17.Langdale JA, Hall LN, Roth R. Control of cellular differentiation in maize leaves. Philosophical Transactions of the Royal Society of London B Biological Sciences. 1995; 350: 53-57.

18.Roth R, Hall LN, Brutnell TP, Langdale JA. bundle sheath defective2, a mutation that disrupts the coordinated development of bundle sheath and mesophyll cells in the maize leaf.The Plant Cell. 1996; 8: 915-927.

19.Park JM, Cho JH, Kang SG, Jang HJ, Pih KT, Piao HL, et al. A dynamin-like protein in Arabidopsis thaliana is involved in biogenesis of thylakoid membranes. EMBO Journal. 1998; 17: 859-867.

20.Kroll D, Meierhoff K, Bechtold N, Kinoshita M, Westphal S, Vothknecht UC, et al. VIPP1, a nuclear gene of Arabidopsis thaliana essential for thylakoid membrane formation. Proceedings of the National Academy of Sciences of the United States of America. 2001; 98: 4238-4242.

21.Osteryoung KW, Pyke KA. Division and Dynamic Morphology of Plastids. Annual Review of Plant Biology. 2014; 65:443-472.

22.Wostrikoff K, Clark A, Sato S, Clemente T, Stern D. Ectopic expression of Rubisco subunits in maize mesophyll cells does not overcome barriers to cell type-specific accumulation. Plant Physiology. 2012; 160: 419-432.

23.Feiz L, Williams-Carrier R, Wostrikoff K, Belcher S, Barkan A, Stern DB. Ribulose-1,5-bis-phosphate carboxylase/oxygenase accumulation factor1 is required for holoenzyme assembly in maize. The Plant Cell. 2012; 24: 3435-3446.

24.Sharwood RE, Ghannoum O, Whitney SM. Prospects for improving CO2 fixation in C3-crops through understanding C4-Rubisco biogenesis and catalytic diversity. Current Opinion in Plant Biology. 2016; 31:135-142.

25.Doron L, Segal N, Gibori H, Shapira M. The BSD2 ortholog in Chlamydomonas reinhardtii is a polysome-associated chaperone that co-migrates on Sucrose gradients with the rbcL transcript encoding the Rubisco large subunit. Plant Journal. 2014; 80: 345-355.

26.Aigner H, Wilson RH, Bracher A, Calisse L, Bhat JY, Hartl FU, et al. Plant RuBisCo assembly in E. coli with five chloroplast chaperones including BSD2. Science. 2017; 358: 1272-1278.

27.Salesse C, Sharwood R, Sakamoto W, Stern D. The Rubisco Chaperone BSD2 May Regulate Chloroplast Coverage in Maize Bundle Sheath Cells. Plant Physiology. 2017; 175: 1624-1633.

28.Eri M, Hiroshi M, Mitsutaka T. Differential positioning of chloroplasts in C4 mesophyll and bundle sheath cells. Plant Signaling & Behavior. 2011; 6: 1111-1113.

29.Feiz L, Williams-Carrier R, Belcher S, Montano M, Barkan A, Stern DB. RAF2: a novel Rubisco biogenesis factor in maize. The Plant Journal. 2014; 80: 862-869.

30.Jang I, Lee K, Nahm B, Kim J. Chloroplast targeting signal of a rice rbcS gene enhances transgene expression. Molecular Breeding. 2002; 9: 81-91.

31.Shen B, Zhu CH, Yao Z, Cui LL, Zhang JJ, Yang CW, He ZH, Peng XX. 2017. An optimized transit peptide for effective targeting of diverse foreign proteins into chloroplasts in rice. Scientific Reports 7, 46231.

32.Chen LT, Hanad S, Fujiwara W, Zhu TH, Thao NP, Wong HL, et al. The Hop/Sti1-Hsp90 chaperone complex facilitates the maturation and transport of a PAMP receptor in rice innate immunity. Cell Host Microbe. 2010; 7: 185–196.

Table 1. Comparison of dividing proplastid number per cell in wild type (WT) and bsd-2 mutant.

dividing proplastid number
		average ± SD (n=20)	p(t-test)
BS	WT	1.46±0.88
			0.001＊＊
	bsd-2	0.49±0.33
M	WT	1.09±0.73
			0.002＊＊
	bsd-2	0.65±0.42

Asterisk means significant difference, ＊＊p＜0.01.

Table 2. Comparison of mature chloroplast number per cell at the maturation stage in wild type (WT) and bsd-2 mutant.

chloroplasts number
		average ± SD (n=20)	p(t-test)
BS	WT	48.6±8.5
	bsd-2		0.000＊＊
	bsd-2	36.3±8.8
M	WT	29.1±4.5
	bsd-2		0.002＊＊
	bsd-2	23.8±5.6

Asterisk means significant difference, ＊＊p＜0.01.

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Cytological evidence of BSD2 functioning in both chloroplast division and dimorphic chloroplast formation in maize leave

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