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Research article
Cytological evidence of BSD2 functioning in both chloroplast division and dimorphic chloroplast formation in maize leaves
Hong Wu
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Background: Maize bsd2 ( bundle sheath defective2 ) is a classical C 4 mutant with defective C 4 photosynthesis, accompanied with reduced accumulation of Rubisco (ribulose bisphosphate carboxylase oxygenase) and aberrant mature chloroplast morphology in the bundle sheath (BS) cells. However, as a hypothetical chloroplast chaperone, the effects of BSD2 on C 4 chloroplast development have not been fully examined yet, which precludes a full appreciation of BSD2 function in C 4 photosynthesis. The aims of our study are to find out the role of BSD2 in regulating chloroplasts development in maize leaves, and to add new insights into our understanding of C 4 biology. Results: We found that at the chloroplast maturation stage, the thylakoid membranes of chloroplasts in the BS and mesophyll (M) cellsbecame significantly looser, and the granaof chloroplasts in the M cells became thinner stacking in the bsd2 mutant when compared with the wildtype plant. Moreover, at the early chloroplast development stage, the number of dividing chloroplasts and the chloroplast division rate are both reduced in the bsd2 mutant, compared with wild type. Quantitative reverse transcriptase-PCR analysis revealed that the expression of both thylakoid formation-related genesand chloroplast division-related genes is significantly reduced in the bsd2 mutants. Further, we showed that BSD2 interacts physically with the large submit of Rubisco (LS) in Bimolecular Fluorescence Complementation assay.
Conclusions: Our combined results suggest that BSD2 plays an essential role in regulating the division and differentiation of the dimorphic BS and M chloroplasts, and that it acts at a post-transcriptional level to regulate LS stability or assembly of Rubisco.
Keywords
Maize, C4 photosynthesis, Kranz anatomy, BSD2, chloroplast division, Rubisco
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CURRENT STATUS: Published
Version 5
Posted 30 Dec, 2019
Published
On 09 Jan, 2020
No community comments so far
Editorial decision: Accept
On 26 Dec, 2019
Editor assigned
On 25 Dec, 2019
Submission checks complete
On 24 Dec, 2019
Editor invited
On 24 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 18 Dec, 2019
Editor assigned
On 17 Dec, 2019
Submission checks complete
On 16 Dec, 2019
Editor invited
On 16 Dec, 2019
No comments provided
Submission checks complete
On 11 Dec, 2019
Editorial decision: Minor revision
On 11 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 06 Dec, 2019
Editor assigned
On 03 Dec, 2019
Submission checks complete
On 02 Dec, 2019
Editor invited
On 02 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 21 Nov, 2019
Review #1 received
Received 15 Nov, 2019
Reviewer #1 agreed
On 01 Nov, 2019
Reviewers invited
Invitations sent on 02 Oct, 2019
Submission checks complete
On 01 Oct, 2019
Editor assigned
On 27 Sep, 2019
Editor invited
On 26 Sep, 2019
First submitted
On 25 Sep, 2019
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Cytological evidence of BSD2 functioning in both chloroplast division and dimorphic chloroplast formation in maize leaves
Hong Wu
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 5
Posted 30 Dec, 2019
Published
On 09 Jan, 2020
No community comments so far
Editorial decision: Accept
On 26 Dec, 2019
Editor assigned
On 25 Dec, 2019
Submission checks complete
On 24 Dec, 2019
Editor invited
On 24 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 18 Dec, 2019
Editor assigned
On 17 Dec, 2019
Submission checks complete
On 16 Dec, 2019
Editor invited
On 16 Dec, 2019
No comments provided
Submission checks complete
On 11 Dec, 2019
Editorial decision: Minor revision
On 11 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 06 Dec, 2019
Editor assigned
On 03 Dec, 2019
Submission checks complete
On 02 Dec, 2019
Editor invited
On 02 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 21 Nov, 2019
Review #1 received
Received 15 Nov, 2019
Reviewer #1 agreed
On 01 Nov, 2019
Reviewers invited
Invitations sent on 02 Oct, 2019
Submission checks complete
On 01 Oct, 2019
Editor assigned
On 27 Sep, 2019
Editor invited
On 26 Sep, 2019
First submitted
On 25 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Background: Maize bsd2 ( bundle sheath defective2 ) is a classical C 4 mutant with defective C 4 photosynthesis, accompanied with reduced accumulation of Rubisco (ribulose bisphosphate carboxylase oxygenase) and aberrant mature chloroplast morphology in the bundle sheath (BS) cells. However, as a hypothetical chloroplast chaperone, the effects of BSD2 on C 4 chloroplast development have not been fully examined yet, which precludes a full appreciation of BSD2 function in C 4 photosynthesis. The aims of our study are to find out the role of BSD2 in regulating chloroplasts development in maize leaves, and to add new insights into our understanding of C 4 biology. Results: We found that at the chloroplast maturation stage, the thylakoid membranes of chloroplasts in the BS and mesophyll (M) cellsbecame significantly looser, and the granaof chloroplasts in the M cells became thinner stacking in the bsd2 mutant when compared with the wildtype plant. Moreover, at the early chloroplast development stage, the number of dividing chloroplasts and the chloroplast division rate are both reduced in the bsd2 mutant, compared with wild type. Quantitative reverse transcriptase-PCR analysis revealed that the expression of both thylakoid formation-related genesand chloroplast division-related genes is significantly reduced in the bsd2 mutants. Further, we showed that BSD2 interacts physically with the large submit of Rubisco (LS) in Bimolecular Fluorescence Complementation assay.
Conclusions: Our combined results suggest that BSD2 plays an essential role in regulating the division and differentiation of the dimorphic BS and M chloroplasts, and that it acts at a post-transcriptional level to regulate LS stability or assembly of Rubisco.
Figure 1
Figure 2
Figure 3
Figure 4