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Original article
Yanjun Dong
Shanghai Normal University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9492-3313
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The chloroplast is a vital photosynthetic organelle for plant growth and development. However, the genetic factors involved in chloroplast development and its relationship with environment factors are largely unknown. Threonyl-tRNA synthetase (ThrRS), one of aminoacyl-tRNA synthetases (AARSs), plays a crucial role in protein synthesis. To date, there are few studies for AARS function on chloroplast development and plant growth, much less ThrRS in rice.
Result
In this paper, we characterized a thermo-sensitive virescent mutant tsv2, which showed albino phenotype and could not survive after the 4-leaf stage when grown at 20 °C, but recovered the normal phenotype when the temperature rose. Map-based cloning and complementation tests showed that TSV2 encoded a chloroplast-located ThrRS protein in rice and the Lys-to-Arg mutation in the anticodon-binding domain affected chloroplast development under cold stress. Furthermore, the loss-of-function of the core domain in TSV2 led to seedling death regardless of temperatures. In addition, TSV2 had a tissue-specific expression, and its disruption resulted in an evidently down-regulation of certain genes associated with chlorophyll biosynthesis, photosynthesis and chloroplast development at cold stress.
Conclusion
The TSV2 encodes a rice threonyl-tRNA synthetase, located in chloroplasts, which is essential for cold-responsive regulation for chloroplast development and plant growth and closely related to the assembly of chloroplast ribosomes and functions at the first step of chloroplast differentiation.
Keywords
Albino phenotype, Chloroplast development, Cold stress, Rice, Threonyl-tRNA synthetase(ThrRS)
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Original article
Yanjun Dong
Shanghai Normal University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9492-3313
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
View the published article at G3 Genes|Genomes|Genetics.
Version 1
Posted 28 Aug, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at G3 Genes|Genomes|Genetics.
Background
The chloroplast is a vital photosynthetic organelle for plant growth and development. However, the genetic factors involved in chloroplast development and its relationship with environment factors are largely unknown. Threonyl-tRNA synthetase (ThrRS), one of aminoacyl-tRNA synthetases (AARSs), plays a crucial role in protein synthesis. To date, there are few studies for AARS function on chloroplast development and plant growth, much less ThrRS in rice.
Result
In this paper, we characterized a thermo-sensitive virescent mutant tsv2, which showed albino phenotype and could not survive after the 4-leaf stage when grown at 20 °C, but recovered the normal phenotype when the temperature rose. Map-based cloning and complementation tests showed that TSV2 encoded a chloroplast-located ThrRS protein in rice and the Lys-to-Arg mutation in the anticodon-binding domain affected chloroplast development under cold stress. Furthermore, the loss-of-function of the core domain in TSV2 led to seedling death regardless of temperatures. In addition, TSV2 had a tissue-specific expression, and its disruption resulted in an evidently down-regulation of certain genes associated with chlorophyll biosynthesis, photosynthesis and chloroplast development at cold stress.
Conclusion
The TSV2 encodes a rice threonyl-tRNA synthetase, located in chloroplasts, which is essential for cold-responsive regulation for chloroplast development and plant growth and closely related to the assembly of chloroplast ribosomes and functions at the first step of chloroplast differentiation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
This is a list of supplementary files associated with this preprint. Click to download.
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