Inuence of Crossbreeding of Ghezel Ram With Grey Shirazi Ewe on Growth Performance, Feed Eciency and Carcass Traits of Fattening Lambs

In this investigation, performance, feed eciency and carcass traits of 48 weaned crossbred Grey Shirazi × Ghezel and purbred Grey Shirazi fattening lambs (mean live weight, 28.5 ± 0.5 kg and age, 115 ± 5 days) were evaluated. Lambs were divided into 4 groups contain the GM group (12 purebred male lambs of Grey Shirazi ewe × Grey Shirazi ram), the GF group (12 purebred female lambs of Grey Shirazi ewe × Grey Shirazi ram), the CM group (12 crossbred male lambs of Grey Shirazi ewe × Ghezel ram) and the CF group (12 crossbred female lambs of Grey Shirazi ewe × Ghezel ram). The dry matter intakes (DMI), average daily gain (ADG) and carcass traits in the lambs were determined. The DMI was greater in CF lambs compared to GF lambs (P < 0.001). Feed conversion ratio (FCR) was higher in male lambs comparing to female lambs (P = 0.001). The nal body weight, ADG, live weight at the slaughtering and hot carcass weight (HCW) were lower in GF lambs compared to another lambs (P < 0.001, P = 0.003). Cold carcass weight (CCW) in CM lambs was higher than in other groups. The Longissimus dorsi (LD) area was higher in crossbreed male lambs (P = 0.001). Crossbreeding improved grow performance (nal body weight and ADG) in male and female lambs. Carcass trait was improved by crossbreeding especially in male lambs. Sex effect on growth performance and carcass traits was noticeable and male lambs had superior growth.


Introduction
The main purpose of sheep breeding is increasing production mostly growth performance and improving reproductive traits (Seyedshari and Hamzehzadeh 2016) and this aim can be improved by genetic, nutrition, health condition and management. Improving of genetic traits of sheep could be done by different methods such as selection, presenting fresh sires, inbreeding, crossbreeding, formation of new breed and blend of these procedures (Notter et al. 2014; Blasco et al. 2019).
Crossbreeding has been experienced for several decades in sheep to create breeds with the best traits (Mahmoud Marai et al. 2009;Caro et al. 2018). This method used to improve animal breeds with high maturity, low feed e ciency and low meat production (Caro-Petrović et al. 2015). Abdullah et al. (2010) showed that crossing Awassi with exotic breeds improved growth performance and productivity.
Similarly, Kutluca Korkmaz and Emsen (2016) reported improvement in growth from crossbreeding of the fat-tailed Morkaraman and Romanov breeds.
Additionally, genotype can be effective on carcass traits of sheep. Crossbreeding by decreasing the total body fat can improve carcass traits of fat tailed breeds (Khaldari and Ghiasi 2018). Results of another study displayed that crossing between Chaal (fat tailed) and Zel (tailed sheep) breeds increased quality of meat and reduced amount of muscular fat compared to pure breeds (Kashan et al. 2005).
The Grey Shirazi sheep is native breed of Fars province of Iran and it is moderate fat-tail in conformation and could be classi ed as a medium breed and has an average body weight of 45-55 kg for ewe and 70-80 kg for ram. This breed has 3 colors, black, brown and gray. Grey lambs are famous for fur production and are slaughtered in age of 3-10 days. However, nowadays these sheep are kept for producing meat (Karimi and Rahmani 2017). This breed is originally a range sheep and well adapted for utilizing low quality feed under adverse environmental conditions in Iran and have a not high growth rate (Makarechian et al. 1978).
Origin of the Ghezel sheep in Iran is Azarbayjan region. This area has arid and cold mountain weather.
This sheep is fat tailed breed and could be classi ed as a heavy breed and has an average body weight of 55-65 kg for ewes and 85-90 kg for rams. This sheep is growing for meat and wool production and the wool color is red and has pieces of dark and bright red. This breed is a farm sheep and has been raised under better environmental condition and has an approximately high growth rate (Nasiriyan et al. 2009;Hosseinzadeh et al. 2016).
Ghezel sheep is classi ed as a heavy breed and have better growth rate compared to Grey Shirazi breed and it was hypothesized that crossbreeding the Gray ewes and Ghezel rams may improve the growth performance of the crossbred offspring. Since the effect of crossbreeding the Grey Shirazi and the Ghezel breeds on male and female lamb's performance has not been investigated yet, this work was done to assess the impact of crossbreeding Grey Shirazi ewe and Ghezel ram breeds on growth performance and carcass traits.

Materials And Methods
Crossbreeding, experimental groups and feeding This investigation was carried out according to The Care and Use of Agricultural Animals in Research and Teaching guidelines at the Fars Agricultural and Natural Resources research and Education Center, Shiraz, Iran.
A total of 180 Grey Shirazi ewes belonging to the Fars Agricultural and Natural Resources research and Education Center, Shiraz, Iran, were used for this study. Two separate place breeding was created. In rst place, breeding between Ghezel rams (n = 6, live weight of 88 ± 3 kg, age average 2.5 ± 0.5 years old) with Grey Shirazi ewes (n = 90, live weight of 45 ± 5 kg, age average 2.5 ± 0.5 years old) and in second place breeding between Grey Shirazi rams (n = 6, live weight of 75 ± 3 kg, age average 2.5 ± 0.5 years old) with Grey Shirazi ewes (n = 90 live weight of 45 ± 5 kg, age average 2.5 ± 0.5 years old) was done. The ratio of ram to ewe was 1:15 in each ock and allowed ability to fresh water and mineral blocks. The concentrate composition was 40% barely grain, 30% corn grain, 27.5% wheat bran, 0.5% calcium carbonate, 1% salt and 1% vitamin mineral premix. After mating, the rams separated and ewes in each ock were kept separately. After lambing, ewes and theirs lambs were numbered and lambs were kept with mothers until weaning. Average birth weight of lambs in purebred and crossbred ocks were 4.5 ± 0.3 kg and 5.0 ± 0.2 kg respectively. Afterwards the lambs in each group were kept in the similar conditions until selection time for present experiment. For the main study, 48 weaned lambs (live weight of 28.50 ± 0.5 kg and 115 ± 5 days of age) (24 male and 24 female lambs) were taken randomly from the each ock and were penned individually in separately cages. Lambs were divided into 4 groups (treatments) (12 lambs per each group). The GM group (12 purebred male lambs of Grey Shirazi ewe × Grey Shirazi ram), GF group (12 purebred female lambs of Grey Shirazi ewe × Grey Shirazi ram), CM group (12 crossbred male lambs of Grey Shirazi ewe × Ghezel ram) and CF group (12 crossbred female lambs of Grey Shirazi ewe × Ghezel ram). Animals were permitted 14 days adaptation. At the beginning of the adaptation diet, all the lambs were cured for external and internal parasites and immunized against enterotoxaemia. The animals were fed to meet requirements according to NRC (2007). The formulated diet contained 13.49% CP and 2.40 Mcal ME/kg DM for ADG of 250 g/d and the percent of forage to concentrate ratio was 47:53 % of DM. The lambs were nourished two times every day (08:15 and 17:15 h) ad libitum to ensure 5% orts and with allowed ability to fresh water and mineral-vitamin bricks. In the all days before morning feed meal, surplus feed of the earlier day was gathered and the orts were measured.

Growth performance
Lambs weight was measured each 15-d intervals during 90 d of the experiment. Body weight was measured for each separate lamb prior to morning feed meal. The ADG was determined for each lamb from day 1 to day 90. Feed conversion ratio (FCR) was calculated as the ratio between DMI and ADG.

Carcass traits
At the nish of the trial days, lambs were weighed after 16 h fasting. Lambs were slaughtered separately by cutting the arteries and the jugular vein in the neck region and were allowed to whole bleeding (Farid 1991). Then the lamb's skins were separated and weights of skin, head and feet were recorded. The internal organs such as rumen, kidneys, lungs, spleen, heart and liver were separated and weighed. The internal fat content was completely separated and weighed. Gut digestive contents were removed and empty body weight was determined by detracting these contents from body weight. The hot carcasses of each lambs were weighed and carcasses were kept at refrigerator (temperature of 4 °C for 24 h). Then weight of cold carcasses was measured. The cold carcasses halved cautiously (Farid 1991). Commercial Iranian cuts performed for halving. The cuts on the left side of each carcass were stored at -20 °C.
The commercial cuts were neck, brisket, thigh, hands, ank, loin, fat tail. For these sections subcutaneous fat in excess of 5 mm was removed, deboned and the weight of each section was recorded. Fat trim and meat tissue from each wholesale cut were combined, ground twice, thoroughly mixed, and samples of approximately 100 g were taken. The samples were packed and frozen at -20 °C for subsequent chemical analysis.
Carcasses were cut between rib 12 and 13 and width and depth of rib eye area (Longissimus dorsi; LD area) were measured on the left cut carcass (Esenbuga et al. 2009). The back-fat thickness was measured in 2 points above section of the LD. The LD area was measured by acetate paper and planimeter. The maximum depth and width of LD was measured by digital coulis. Tight, hand, neck, loin, brisket, ank, fat tail, total fat half-carcass without fat tail, total fat half-carcass with fat tail, leans and bones were separated and weighed (Farid 1991).

Laboratory analyses
The samples of consumed ration and orts were dried by oven at 60 °C for 48 h and were ground in size of 1 mm. Dry matter of samples was measured using oven drying at 105 °C for 16 h (AOAC 1990; method 930.15). The amount of Ash was measured using burning of samples at 550 °C for 12 h. Organic matter content was calculated as difference between sample weight and ash content (AOAC 1990; method 942.05). The NDFom was determined by method of Van Soest et al. (1991). ADFom content was determined according to AOAC (1990; method 973.18). Lignin was measured by solubilization of cellulose with sulfuric acid (Robertson and Van Soest 1981). Nitrogen content was measured by the Kjeldahl device (AOAC 1990; method 954.01).
For meat chemical analysis, moisture, ash, ether extract (EE) and crude protein (CP) were determined according to the AOAC (1990) methods.

Statistical analyses
Data were analyzed using the general linear model with a completely randomized design considering genotype, sex and genotype × sex interaction as variables. Data was analyzed by the SAS (2001) software and using the initial weight as a covariate. The means treatments were compared by Tukey's test with 5% probability level.
Where Y ijkl is the overall observation, μ the overall mean, G i the ith effect of genotype, S j, the jth effect of sex, GS ij the interaction of genotype and sex, 1(Cov)m the covariate effect and e ijkl the standard error term.

Growth performance and dry matter intake
The voluntary consumption of DM was signi cantly higher in CM and CF compared to GM and GF (P < 0.0001) ( Table 2) but difference in male and female lambs was not signi cant. The nal BW and ADG in CM and CF lambs were higher than those for GM and GF lambs (P = 0.0005 and P = 0.002 respectively) ( Table 2). The nal BW among crossbred male lambs was higher than crossbred female lambs (P = 0.011) but among purebred male and female lambs was not different. The ADG between CM and CF and between GM and GF was different and in males lambs was higher than female lambs (P = 0.003). The FCR between CM and GM lambs and between CF and GF lambs was not different; while, this parameter was lower in CM and GM lambs comparing to CF and GF lambs (P < 0.0001).

Carcass traits data of crossbreed and non-crossbreed lambs
The HCW in CM lambs was higher compared to GM lambs (P = 0.0007) but this weight was not different between CF and GF. The HCW in crossbred male lambs was greater compared to crossbred female lambs (P = 0.021). The amount of CCW in CM and CF lambs was greater than in GM and GF groups (P = 0.001).
The CCW in crossbred male lambs was greater compared to crossbred female lambs (P = 0.025). In GF and CF lambs, IFW was higher than in GM and CM lambs (P = 0.0001). Internal fat weight as percent of live body weight in female lambs (GF and CF) was higher than in male lambs (GM and CM) (P < 0.0001). Total of hard drops and components containing hide, head and feet in female lambs (GF and CF) were lower than in male lambs (GM and CM) (P < 0.0001) but noteworthy difference between GM and CM lambs and between GF and CF lambs was not observed. Weight of head in GM lambs was higher than CM lambs. Total of soft drops (P = 0.0002) and components containing kidneys (P = 0.0001) and liver (P < 0.0001) in female lambs (GF and CF) were lower than in male lambs (GM and CM). Weight of spleen and heart in GM lambs were lower than CM lambs (P = 0.003 and P < 0.0001 respectively). Thigh, hand and loin weights in GM was lower than in CM (P = 0.003, P = 0.004 and P = 0.005 respectively) but difference between GF and CF was not signi cant for this parameters. Weights of brisket and ank and thigh, hand, loin and brisket weights as a percent of cold half-carcass were not different among experimental lambs. Neck and Neck weight as a percentage of cold half-carcass weight in GM lambs were lower than in CM lambs but these parameters had no signi cant difference in GF and CF lambs. Neck and Neck weight as a percentage of cold half-carcass weight in crossbred male lambs were higher than their females but these parameters in purebred male and female lambs were not different.
Lean weight only in purebred and crossbred male lambs was different (P = 0.0006) but in female lambs was not diverse. Lean weight and lean weight as a percentage of cold half-carcass weight in crossbred male lambs were greater than female lambs (P = 0.0004 and P = 0.004 respectively). Bone weight in GM and GF lambs was lower than CM and CF lambs (P = 0.0003). The LD area (cm 2 ) was higher in crossbreed male lambs comparing to the purebred male lambs (P = 0.025) but was not signi cantly different in purebred and crossbred female lambs. This area in CF lambs was lower than CM lambs (P = 0.0002). Weights of brisket, ank, fat tail and fat thickness and weights of thigh, hand, loin, brisket, fat tail, total fat half-carcass with fat tail and bone as a percentage of cold half-carcass weight were not diverse among experimental lambs. Meat chemical composition measured of the purebred and crossbred lambs was presented at Table 5. The DM, CP, Ash and CF of meat in purebred and crossbred lambs were not different. Protein content of meat in CM lambs was higher than CF lambs (P = 0.013) but meat Ash content in CM lambs was lower than CF lambs (P = 0.001). Dry matter and Ash content of meat in GF lambs were greater than CF lambs (P < 0.0001 and P = 0.001 respectively).

Discussion
Growth performance and dry matter intake Crossbreeding had noticeable impact on feed intake for both male and female because of effect of the hybrid vigor that may occur in the F1 generation. Crossbreeding Lori-Bakhtiari and Romanov (Khaldari and Ghiasi 2018) and Awassi and Charollais (Abdullah et al. (2010) improved feed intake of the lambs, but Hassan et al. (1996) and Talebi and Gholamhosani (2017) indicated that DMI was not affected by crossbreeding. The interaction effect between genotype and sex for feed intake was signi cant and it Page 7/21 shows that sex can be affected by genotype effect. Therefore by crossbreeding, DMI in crossbred female lambs (CF) increased compared to purebred male and female lambs (GM and GF). The ADG in experimental male lambs was in the range of 203.19-242.42 g/d in our study which was in line to lambs (211-240 g/d) (Eilami 2008). The advantage of crossbreeding compared to pure breeding for animal growth and feed conversion ratio has been reported in many studies (Abdullah et al. 2010; Kutluca Korkmaz and Emsen 2016). In our study crossbreeding had positive in uence on nal BW and ADG in male and female lambs and crossbred male and female lambs had 10.3% and 9.65% higher nal weight and 20.9% and 40% more ADG compared to purebred male and female lambs. Results of another research indicated about 25% and 23% increase for ADG in crossbred lambs compared to the purebred lambs (Fathala et al. 2014;Dvalishvili et al. 2015). In contrast to our results, Khaldari and Ghiasi (2018) described that purebred lambs of Lori-Bakhtiari had more nal BW and ADG comparing to the crossbreed lambs Lori-Bakhtiari with Romanov breed. It appears that the preference of crossbred fattening lambs compared to purebred probably depends on the in uence of heterosis (hybrid vigor), breed complementarity and the puberty weight

Carcass Traits Data Of Crossbreed And Non-crossbreed Lambs
With regarding to the any effect of crossbreeding on total hard and soft drops (Table 3), improvement in HCW and CCW in crossbred compared to purebred lambs can be directly by carcass weight increment.  (Farid 1991). In our study, crossbred and purebred lambs had any different for IFW, IFW as a percentage of live body weight, fat tail weight, fat tail weight as a percentage of cold half-carcass, but crossbreeding decreased fat tail compared to Ghezel male lambs in study by Izedifard and Dadpasand (2009). Similar to our work, Izedifard and Dadpasand (2009) reported that IFW, IFW as a percentage of live body weight, fat tail weight, fat tail weight as a percentage of cold half-carcass weight in crossbred and purebred lambs of Ghezel and Mehraban breed were not different. In contrast to our result, Khaldari and Ghiasi (2018) concluded that purebred lambs of Lori-Bakhtiari had heavier fat tail and total fat associated to the crossbreed lambs Lori-Bakhtiari with Romanov breed. Additionally, it has been shown in some research works that crossbreeding decreased total body fat content compared to the pure breeding ( Total hard drops, hide and feet were not affected by crossbreeding because the most effect of crossbreeding was on carcass gain. Similarly, in other works reported that weight of hard drops were not different in purebred and crossbred lambs (Kashan et al. 2005;Ünal et al. 2006). However, it has been reported in another work that weights of feet and hide in crossbred lambs were lesser than those of purebred lambs (Khaldari and Ghiasi 2018). Sex of lamb's effect on weight of total hard drops, head, hide and feet and heavier weight of these organs in male lambs can be due to more growth of males. In consist other studies had shown higher weight of hard drop in male lambs related to female lambs Weight of total soft drops not affected by crossbreeding except for heart and spleen weight which was greater in crossbred male lambs in comparing to purebred male lambs. Our results were like to the some other studies (Kashan et al. 2005;Tsegay et al. 2013;Khaldari and Ghiasi 2018). It has been reported in some research works that heart weight is affected by the lamb's genotype and in crossbred lambs is higher than in pure lambs (Kashan et  Crossbreeding increased thigh, hand, loin and neck weight (kg) in male lambs but had no effect on female lambs. Similarly Seyedshari and Hamzehzadeh (2016) described that crossbred lambs of Chaal and Varamini had higher hand weight compared to Varamini pure lambs. However, weights of brisket and ank were not affected by crossbreeding. Similar to our result, other studies also reported lack of in uence of crossbreeding on these carcass characteristics (Ünal et al. 2006;Abdullah et al. 2010;Khaldari and Ghiasi 2018). In contrast, Kashan et al. (2005) showed that crossbred lambs had higher weight of brisket and shoulder compared to the pure lambs.
Effect of sex on loin and neck weight was signi cant in crossbred lambs so that these weights in male lambs were higher than in female lambs. Sex associated differences in these weights may be due to On the other hand, weights of thigh, brisket and ank were not affected by sex. In contrast to our result, it has been reported in some studies that sex effect on these carcass characteristic was important and in male lambs was greater than in female lambs (Malik et al. 1996; Seyedshari and Hamzehzadeh 2016). Crossbred male lambs had more lean weight (16.13%) than purebred lambs and crossbred male and female lambs have approximately 11.23% and 19% more bone than purebred lambs in due to hybrid vigor. Similar to our result, Seyedshari and Hamzehzadeh (2016) and Khaldari and Ghiasi (2018) showed that crossbred lambs had greater lean meat compared to the purebred lambs but bone weight was not affected by crossbreeding. However, Kashan et al. (2005) illustrated that lean meat in purebred Chaal and Zandi lambs was similar to crossbred Zel × Chaal and Zel × Zandi lambs but bone weight was lower in purebred Chaal and Zandi compared to crossbred Zel × Chaal lambs. The fat thickness was not different among the experimental lambs. Similarly, Vargas Junior et al. Crossbreeding had no effect on DM, CP, Ash and EE of male and female lamb's meat. Effect of crossbreeding on meat chemical composition among studies is different. In another work had shown that DM, CP and Ash of meat in purebred and crossbred lambs were not different but purebred lambs had the lowest intramuscular fat percentage compared to crossbred lambs (Abdullah et al. 2010). Kashan et al. (2005) described that the percentage of CP in the meat of crossbred lambs was greater than purebred lambs but EE% was lower and DM and Ash percentage were not different. Sex dependent effects on meat chemical composition were observed in lambs, with the exception of fat content, in disagreement with Rodríguez et al. (2011). Testosterone hormone, besides providing an anabolic in uence acts on plasma glucose concentrations in males but does not modify the phosphorylation of AMP-activated protein kinase in muscle, which effects the concentration of intramuscular fat in males to a minor amount. A parallel result was detected in this study because the percentage of meat fat was similar between the male and female sexes (Clarke et al. 2012;Araújo et al. 2017).

Conclusions
Crossbreeding Grey Shirazi ewes with Ghezel rams improved dry matter intake and growth performance in male and female lambs. Some of Carcass trait was improved by crossbreeding especially in male lambs. Sex effect on growth performance and carcass traits was noticeable and male lambs had superior growth. However it is suggested that in future studies effects of breeding between Grey Shirazi ram and Ghezel ewe would be tested.

Declarations Ethical Approval
This study was approved by the Livestock Research Team at Investigation Organization of Animal Science, Iran and was conducted according to the guidelines of care and using animals in research work.

Consent to Participate
All authors agree to participate in the current work.  Tables   Table 1 Ingredients and chemical composition of the experimental diet 1