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Research Article
Fengjuan Zhang
Hebei University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7131-2592
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Aims
Bacillus, a gram-positive bacterium, has multiple beneficial traits which help the plants in nutrients acquisition, either directly or indirectly. However, the mechanisms that mediate the positive or negative impact of Bacillus on exotic or native species are poorly understood. Our objective was to determine whether the quantitative and/ or qualitative differences in the Bacillus community present on the exotic Ambrosia artemisiifolia and the native Setaria viridis provide a competitive advantage to the invader over native species.
Methods
A. artemisiifolia monoculture, mixture of A. artemisiifolia and S. viridis and S. viridis monoculture were designed in the field experiment. Bacillus diversity in their rhizospheres was analyzed using 16S rRNA and their effects on the competitive growth of A. artemisiifolia and S. viridis were tested in greenhouse experiment.
Results
The Shannon index, species richness, and evenness index of Bacillus diversity in the rhizosphere soil of A. artemisiifolia in the monoculture treatment were lower than in the mixture treatment. The relative abundance of Bacillus megaterium in the rhizosphere soil of A. artemisiifolia was higher than that in the rhizosphere soil of S. viridis. Whether Bacillus in the rhizosphere soil of A. artemisiifolia or B. megaterium inoculation enhanced the relative competitiveness of A. artemisiifolia and inhibited that of S. viridis by altering their carbon, nitrogen, and phosphorus concentrations.
Conclusions
A. artemisiifolia invasion influenced Bacillus communities, especially B. megaterium. The higher abundance of B. megaterium in A. artemisiifolia rhizosphere creates higher levels of the available nutrient than that in native S. viridis.
Keywords
Bacillus, rhizosphere soil, Ambrosia artemisiifolia, competitive growth nutrient
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- Slide10.png
Fig. S1 Neighbor-joining tree showing phylogeny of representative Bacillus DNA sequences isolated from root and reference sequences from GeneBank. Fungal sequences from this study are identified by their GenBank sequences beginning with MK463590. Closely aligned sequences from GenBank are identified by the species name or accession number. Scutellospora alterata is used as the out-group (left panel). Relative abundance of each AMF type detected from root sampled from different treatments (right panel). Treatments: A (A.artemisiifolia monoculture), A(M) (A. artemisiifolia in the mixture of A. artemisiifolia and S. viridis), S (S. viridis monoculture), S(M) (S. viridis in the mixture of A. artemisiifolia and S. viridis).
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This preprint is under consideration at Plant and Soil. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Fengjuan Zhang
Hebei University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7131-2592
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 24 Jun, 2021
No community comments so far
Reviews received
Received 25 Jun, 2021
Reviewers invited
Invitations sent on 21 Jun, 2021
Editor invited
On 20 Jun, 2021
Editor assigned
On 20 Jun, 2021
First submitted
On 20 Jun, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Aims
Bacillus, a gram-positive bacterium, has multiple beneficial traits which help the plants in nutrients acquisition, either directly or indirectly. However, the mechanisms that mediate the positive or negative impact of Bacillus on exotic or native species are poorly understood. Our objective was to determine whether the quantitative and/ or qualitative differences in the Bacillus community present on the exotic Ambrosia artemisiifolia and the native Setaria viridis provide a competitive advantage to the invader over native species.
Methods
A. artemisiifolia monoculture, mixture of A. artemisiifolia and S. viridis and S. viridis monoculture were designed in the field experiment. Bacillus diversity in their rhizospheres was analyzed using 16S rRNA and their effects on the competitive growth of A. artemisiifolia and S. viridis were tested in greenhouse experiment.
Results
The Shannon index, species richness, and evenness index of Bacillus diversity in the rhizosphere soil of A. artemisiifolia in the monoculture treatment were lower than in the mixture treatment. The relative abundance of Bacillus megaterium in the rhizosphere soil of A. artemisiifolia was higher than that in the rhizosphere soil of S. viridis. Whether Bacillus in the rhizosphere soil of A. artemisiifolia or B. megaterium inoculation enhanced the relative competitiveness of A. artemisiifolia and inhibited that of S. viridis by altering their carbon, nitrogen, and phosphorus concentrations.
Conclusions
A. artemisiifolia invasion influenced Bacillus communities, especially B. megaterium. The higher abundance of B. megaterium in A. artemisiifolia rhizosphere creates higher levels of the available nutrient than that in native S. viridis.
Figure 1
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Figure 3
Figure 4
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