See the published version of this preprint at BMC Genomics.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research article
Shaojun Dai
Shanghai Normai University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8063-6946
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Salinity has obvious effects on plant growth and crop productivity. The salinity-responsive mechanisms have been well-studied in differentiated organs (e.g., leaves, roots and stems), but not in unorganized cells such as callus. High-throughput quantitative proteomics approaches have been used to investigate callus development, somatic embryogenesis, organogenesis, and stress response in numbers of plant species. However, they have not been applied to callus from monocotyledonous halophyte alkaligrass (Puccinellia tenuifora).
Results: The alkaligrass callus growth, viability and membrane integrity were perturbed by 50 mM and 150 mM NaCl treatments. Callus cells accumulated the proline, soluble sugar and glycine betaine for the maintenance of osmotic homeostasis. Importantly, the activities of ROS scavenging enzymes (e.g., SOD, APX, POD, GPX, MDHAR and GR) and antioxidants (e.g., ASA, DHA and GSH) were induced by salinity. The abundance patterns of 55 salt-responsive proteins indicate that salt signal transduction, cytoskeleton, ROS scavenging, energy supply, gene expression, protein synthesis and processing, as well as other basic metabolic processes were altered in callus to cope with the stress.
Conclusions: The undifferentiated callus exhibited unique salinity-responsive mechanisms for ROS scavenging and energy supply. Activation of the POD pathway and AsA-GSH cycle was universal in callus and differentiated organs, but salinity-induced SOD pathway and salinity-reduced CAT pathway in callus were different from those in leaves and roots. To cope with salinity, callus mainly relied on glycolysis, but not the TCA cycle, for energy supply.
Keywords
Salt response, ROS scavenging, Energy supply, Osmotic homeostasis, Callus, Halophyte alkaligrass, Proteomics
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Genomics.
No community comments so far
Editorial decision: Accept
On 22 Nov, 2019
Editor assigned
On 21 Nov, 2019
Submission checks complete
On 20 Nov, 2019
Editor invited
On 20 Nov, 2019
Version 2
Posted 12 Nov, 2019
No comments provided
Editorial decision: Minor revision
On 18 Nov, 2019
Review #1 received
Received 15 Nov, 2019
Review #2 received
Received 13 Nov, 2019
Reviewer #2 agreed
On 06 Nov, 2019
Editor assigned
On 05 Nov, 2019
Reviewers invited
Invitations sent on 05 Nov, 2019
Reviewer #1 agreed
On 05 Nov, 2019
Submission checks complete
On 04 Nov, 2019
Editor invited
On 04 Nov, 2019
No comments provided
Review #2 received
Received 30 Oct, 2019
Editorial decision: Minor revision
On 30 Oct, 2019
Review #1 received
Received 28 Oct, 2019
Reviewer #2 agreed
On 20 Oct, 2019
Reviewers invited
Invitations sent on 09 Oct, 2019
Reviewer #1 agreed
On 09 Oct, 2019
Submission checks complete
On 03 Oct, 2019
Editor invited
On 03 Oct, 2019
Editor assigned
On 30 Sep, 2019
First submitted
On 27 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Genomics.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Shaojun Dai
Shanghai Normai University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8063-6946
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Genomics.
No community comments so far
Editorial decision: Accept
On 22 Nov, 2019
Editor assigned
On 21 Nov, 2019
Submission checks complete
On 20 Nov, 2019
Editor invited
On 20 Nov, 2019
Version 2
Posted 12 Nov, 2019
No comments provided
Editorial decision: Minor revision
On 18 Nov, 2019
Review #1 received
Received 15 Nov, 2019
Review #2 received
Received 13 Nov, 2019
Reviewer #2 agreed
On 06 Nov, 2019
Editor assigned
On 05 Nov, 2019
Reviewers invited
Invitations sent on 05 Nov, 2019
Reviewer #1 agreed
On 05 Nov, 2019
Submission checks complete
On 04 Nov, 2019
Editor invited
On 04 Nov, 2019
No comments provided
Review #2 received
Received 30 Oct, 2019
Editorial decision: Minor revision
On 30 Oct, 2019
Review #1 received
Received 28 Oct, 2019
Reviewer #2 agreed
On 20 Oct, 2019
Reviewers invited
Invitations sent on 09 Oct, 2019
Reviewer #1 agreed
On 09 Oct, 2019
Submission checks complete
On 03 Oct, 2019
Editor invited
On 03 Oct, 2019
Editor assigned
On 30 Sep, 2019
First submitted
On 27 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Genomics.
Background: Salinity has obvious effects on plant growth and crop productivity. The salinity-responsive mechanisms have been well-studied in differentiated organs (e.g., leaves, roots and stems), but not in unorganized cells such as callus. High-throughput quantitative proteomics approaches have been used to investigate callus development, somatic embryogenesis, organogenesis, and stress response in numbers of plant species. However, they have not been applied to callus from monocotyledonous halophyte alkaligrass (Puccinellia tenuifora).
Results: The alkaligrass callus growth, viability and membrane integrity were perturbed by 50 mM and 150 mM NaCl treatments. Callus cells accumulated the proline, soluble sugar and glycine betaine for the maintenance of osmotic homeostasis. Importantly, the activities of ROS scavenging enzymes (e.g., SOD, APX, POD, GPX, MDHAR and GR) and antioxidants (e.g., ASA, DHA and GSH) were induced by salinity. The abundance patterns of 55 salt-responsive proteins indicate that salt signal transduction, cytoskeleton, ROS scavenging, energy supply, gene expression, protein synthesis and processing, as well as other basic metabolic processes were altered in callus to cope with the stress.
Conclusions: The undifferentiated callus exhibited unique salinity-responsive mechanisms for ROS scavenging and energy supply. Activation of the POD pathway and AsA-GSH cycle was universal in callus and differentiated organs, but salinity-induced SOD pathway and salinity-reduced CAT pathway in callus were different from those in leaves and roots. To cope with salinity, callus mainly relied on glycolysis, but not the TCA cycle, for energy supply.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
Loading...