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Original article
Wenming Wang
Sichuan Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3450-8422
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases of rice. An increasing number of microRNAs (miRNAs) have been reported to fine-tune rice immunity against M. oryzae and coordinate with growth and development.
Results
Here, we showed that rice microRNA159a (Osa-miR159a) played a positive role in rice resistance to M. oryzae. The expression of Osa-miR159a was suppressed in a susceptible accession at 12, 24, and 48 hours post-inoculation (hpi); it was upregulated in a resistant accession of M. oryzae at 24 hpi. The transgenic rice lines overexpressing Osa-miR159a were highly resistant to M. oryzae. In contrast, the transgenic lines expressing a short tandem target mimic (STTM) to block Osa-miR159 showed enhanced susceptibility. Knockout mutations of the target genes of Osa-miR159, including OsGAMYB, OsGAMYBL, and OsZF, led to resistance to M. oryzae. Alteration of the expression of Osa-miR159a impacted yield traits including pollen and grain development.
Conclusions
Our results indicated that Osa-miR159a positively regulated rice immunity against M. oryzae by downregulating its target genes. Proper expression of Osa-miR159a was critical for coordinating rice blast resistance with grain development.
Keywords
microRNA, Osa-miR159a, short tandem target mimic, Magnaporthe oryzae, rice blast disease
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CURRENT STATUS: Published
View the published article at Rice.
No community comments so far
Editorial decision: Minor revision
On 21 Jan, 2021
Review #2 received
Received 06 Jan, 2021
Reviewer #2 agreed
On 26 Dec, 2020
Reviewer #1 agreed
On 26 Dec, 2020
Review #1 received
Received 26 Dec, 2020
Reviewers invited
Invitations sent on 24 Dec, 2020
Editor assigned
On 09 Dec, 2020
Submission checks complete
On 09 Dec, 2020
Editor invited
On 09 Dec, 2020
Version 1
Posted 01 Sep, 2020
No comments provided
Editorial decision: Major revision
On 20 Oct, 2020
Review #2 received
Received 15 Oct, 2020
Reviewer #2 agreed
On 27 Sep, 2020
Review #1 received
Received 11 Sep, 2020
Reviewers invited
Invitations sent on 06 Sep, 2020
Reviewer #1 agreed
On 06 Sep, 2020
First submitted
On 27 Aug, 2020
Editor assigned
On 27 Aug, 2020
Submission checks complete
On 26 Aug, 2020
Editor invited
On 26 Aug, 2020
Metrics
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A new preprint design is coming!
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See the published version of this preprint at Rice.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Original article
Wenming Wang
Sichuan Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3450-8422
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Rice.
No community comments so far
Editorial decision: Minor revision
On 21 Jan, 2021
Review #2 received
Received 06 Jan, 2021
Reviewer #2 agreed
On 26 Dec, 2020
Reviewer #1 agreed
On 26 Dec, 2020
Review #1 received
Received 26 Dec, 2020
Reviewers invited
Invitations sent on 24 Dec, 2020
Editor assigned
On 09 Dec, 2020
Submission checks complete
On 09 Dec, 2020
Editor invited
On 09 Dec, 2020
Version 1
Posted 01 Sep, 2020
No comments provided
Editorial decision: Major revision
On 20 Oct, 2020
Review #2 received
Received 15 Oct, 2020
Reviewer #2 agreed
On 27 Sep, 2020
Review #1 received
Received 11 Sep, 2020
Reviewers invited
Invitations sent on 06 Sep, 2020
Reviewer #1 agreed
On 06 Sep, 2020
First submitted
On 27 Aug, 2020
Editor assigned
On 27 Aug, 2020
Submission checks complete
On 26 Aug, 2020
Editor invited
On 26 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Rice.
Background
Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases of rice. An increasing number of microRNAs (miRNAs) have been reported to fine-tune rice immunity against M. oryzae and coordinate with growth and development.
Results
Here, we showed that rice microRNA159a (Osa-miR159a) played a positive role in rice resistance to M. oryzae. The expression of Osa-miR159a was suppressed in a susceptible accession at 12, 24, and 48 hours post-inoculation (hpi); it was upregulated in a resistant accession of M. oryzae at 24 hpi. The transgenic rice lines overexpressing Osa-miR159a were highly resistant to M. oryzae. In contrast, the transgenic lines expressing a short tandem target mimic (STTM) to block Osa-miR159 showed enhanced susceptibility. Knockout mutations of the target genes of Osa-miR159, including OsGAMYB, OsGAMYBL, and OsZF, led to resistance to M. oryzae. Alteration of the expression of Osa-miR159a impacted yield traits including pollen and grain development.
Conclusions
Our results indicated that Osa-miR159a positively regulated rice immunity against M. oryzae by downregulating its target genes. Proper expression of Osa-miR159a was critical for coordinating rice blast resistance with grain development.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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