We present the MR properties of a novel bio-responsive phosphorus probe doped with iron for dual proton and phosphorus magnetic resonance imaging (1H/31P-MRI), which provide simultaneously complementary information. The probes consist of non-toxic biodegradable calcium phytate (CaIP6) nanoparticles doped with different amounts of cleavable paramagnetic Fe3+ ions. Phosphorus atoms in the phytate structure delivered an efficient 31P-MR signal, with iron ions altering MR contrast for both 1H and 31P-MR. The coordinated paramagnetic Fe3+ ions also broadened the 31P-MR signal spectral line due to the short T2 relaxation time, making it undetectable at higher concentrations. However, when Fe3+ was decomplexed from the probe, relaxation times were prolonged. As a result, 1H-MR contrast changes as well as the 31P-MR signal increase. These 1H and 31P-MR dual signals triggered by iron decomplexation may have been attributable to biochemical changes in the environment with strong iron chelators, such as bacterial siderophore (deferoxamine). Analysing MR signal alternations as a proof-of-principle on a phantom at a 4.7 T magnetic field, we found that iron presence influenced 1H and 31P signals and signal recovery via iron chelation using deferoxamine.
Research Article
Iron-doped Calcium Phytate Nanoparticles as a Bio-responsive Contrast Agent in 1H/31P Magnetic Resonance Imaging
https://doi.org/10.21203/rs.3.rs-669005/v1
This work is licensed under a CC BY 4.0 License
Journal Publication
published 08 Feb, 2022
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Iron-doped
calcium phytate nanoparticles
bio-responsive contrast agent
Magnetic resonance (MR)
1H/31P
magnetic resonance imaging
Magnetic resonance (MR), which comprises non-invasive spectroscopy (MRS) and imaging (MRI), is a widely used method in a number of medical settings and applications. Noted for its high resolution, excellent soft tissue contrast and non-ionising radiation, MR has distinct advantages over other in vivo imaging techniques. While the use of contrast agents and tracers makes the method more sensitive and specific, biocompatibility-related issues can arise1. A number of contrast agents have been proposed, notably non-toxic probes that are responsive to various biochemical stimuli, tissue-specific targeting and theranostics2,3,4. One of the most effective methods is non-hydrogen X-nuclei MR imaging, which provides complementary information to anatomical 1H-MR imaging and visualisation of physiological processes5,6,7, even on a cellular level8. Phosphorus is one of the more promising X-nuclei and the second most commonly used nucleus in biological MR studies. Interest in phosphorus MR stems from its ability to provide unique information on the composition of cell membranes, bioenergetics, oxidative phosphorylation, intracellular pH and magnesium levels9,10,11,12. Most common phosphorus-containing compounds measured by in vivo 31P-MR, like adenosine triphosphate (ATP) and phosphocreatine (PCr), can generate a chemical shift of 0 ± 30 ppm13. Phosphorus contains the stable natural monoisotope 31P, which possesses a non-zero magnetic moment (spin = ½) and a gyromagnetic ratio of 17.2 MHz T− 1 (about 60 % lower than that of ¹H). Completely abundant in nature, phosphorous is detectable even at the low magnetic fields typically used in clinical practice. Positioning various paramagnetic (Mn2+, Fe3+ or Gd3+) or superparamagnetic (SPION) ions in the vicinity of the phosphorus nucleus can significantly affect relaxation times and, as a result, change the contrast properties of both 1H14,15 and 31P MR images. In cases of extremely short T2 relaxation times, the MR signal may disappear completely. In this article, we present a novel magnetic resonance contrast agent based on calcium phytate (CaIP6) nanoparticles doped with paramagnetic iron irons (Fe3+) (for the chemical properties, see16). Compared with other ions used in MR probes, such as Gd3+, the biogenic Fe3+ ion boasts highly regulated naturally occurring systems and ensures safe transport and storage throughout the organism17, thus reducing any long-term toxic effects.
The non-toxic nature of the probe is crucial for its application in medicine, including therapy18 and diagnosis. Some phosphorus-containing organic materials are considered highly biocompatible19 and thus suitable for medical use. One of the more promising of the phosphorus-based compounds for medical settings is phytic acid (IP6)20. Also known as myo-inositol-1,2,3,4,5,6-hexakisphosphate, IP6 is a fully phosphorylateed derivative of inositol that occurs naturally in plants, especially in seeds and grains. The profuse presence of phytate in biological systems means it is highly biocompatible21, as confirmed by the use of technetium-99m (99mTc-phytate) in in vivo diagnostics22. Comprising six negatively charged phosphate groups, IP6 is adept at capturing cations. It also inhibits iron-mediated generation of the highly toxic hydroxyl radical23, chelating polyvalent metal cations and preventing its uptake in the digestive tract24. The resulting ablation of the system significantly reduces pathogen’s ability to colonise hosts25. This reduction in iron uptake is compensated for by siderophores26,27, which are compounds capable of capturing iron and distributing it to microbial cells28 and a number of pathogens during infection. Siderophores are relatively low molecular weight substances that act as chelating agents for iron ions produced by pathogenic, as well as non-pathogenic bacteria and fungi generated during iron deficiency. While iron-chelating siderophores produced by bacteria display high affinity for Fe3+ ions, nanosized siderophore-containing substances can also be used to accelerate the complexation of iron, as in a previous physiological stimulation model16, the iron-binding bacterial siderophore deferoxamine (DFOA), an FDA-approved small molecule drug used in iron overload treatment29 originating from the bacteria Streptomyces pilosus. Siderophores are also useful in diagnosing bacterial diseases30,31,32, including infections of the gastrointestinal tract and those associated with local-use implants. The iron-chelating ability of siderophores was also recently investigated in relation to malignant cancerous cells33 and in antibiotic-siderophore conjugates34.
Calcium phytate nanoparticles doped with low concentrations of Fe3+ ions have been shown to be non-toxic, a precondition for in vivo application. Due to the presence of paramagnetic Fe3+ ions, the nanoparticles act primarily as a T2 contrast agent in 1H-MR. Short relaxation times provide a hypointense contrast on T2-weighted 1H-MR images while simultaneously broadening the 31P peak leading the phosphorus MR signal to a noise level, making it impossible to detect at higher iron concentrations. On the other hand, the phosphorus MR signal recovers when Fe3+ concentration in the phytate complex is lower.
In this proof-of-principle phantom study, we investigated the effect of different iron concentrations on the proton and phosphorus MR signals of calcium phytate at a 4.7 T magnetic field. We also evaluated the MR sensitivity and MR properties of the probe, tested its biocompatibility and visualize recovery of the MR signal in a chelation model using the iron-binding bacterial siderophore DFOA.
1H/31P magnetic resonance coil homogeneity
A field of view providing sufficient image quality was determined for further implementation of the coil. The working area of the radiofrequency (RF) coil is presented with signal attenuation (∆5 dB) to indicate areas of high sensitivity measurement. Inhomogeneity in the image, which reflects the positioning of the RF conductors, is characteristic of solenoid coils. The coil enables high signal-to-noise (SNR; Eqs. 1 and 2) measurement, which is crucial for 31P MR at low magnetic fields. The working area and sensitivity of the RF coil are visualised in Fig. 1.
1H magnetic resonance of phantoms
1H-MRI of phantoms containing high amounts of iron appeared darker on T2-weighted images compared to images with lower iron content (Fig. 2a). The lowest SNR = 1.2 issued from the probe doped with the highest Fe3+ concentration and, as expected, the highest SNR = 86.2 issued from the probe containing no iron. Reflecting the effect of iron chelation, the probe with added deferoxamine (DFOA; 2.73 mmol L− 1 Fe3++DFOA: SNR = 34.7) provided a 36 %-higher 1H-MR signal compared to its pre-chelation form (2.73 mmol L− 1 Fe3+: SNR = 25.4). The sensitivity curve reflected the signal intensity (SI) of MR dependence on iron concentration (Fig. 3a). The 1H image contrast was affected even at low iron concentrations (starting with 0.68 mmol L− 1 Fe3+), confirming the effectiveness of the probe as a T2 contrast agent. Imaging results were supported by relaxometry findings; both T1 and T2 relaxation times decreased with increasing iron concentration, resulting in T2 = 22.2 ms for 2.73 mmol L− 1 Fe3+ and T2 = 33.9 ms for the iron-free probe. Results are summarised in Fig. 2, Table 1 and Table 2.
31P magnetic resonance of phantoms
The 31P-MRI signal with the highest SNR = 41.8 in the iron-free probe decreased as iron content in the probe increased (Fig. 2b) until the signal was no longer detectable at 5.43 mmol L− 1 Fe3+. Additionally, coordinated iron ions affected 31P-MRS signal broadening in correlation with increasing Fe3+ concentration; no signal was obtained from the highest concentrations ≥ 5.43 mmol L− 1 Fe3+ in accordance with 31P and 1H-MRI results. Iron chelation was visible in 31P-MRS of the 2.73 mmol L− 1 Fe3+ probe with added DFOA, showing an increased signal (SNR = 72.2) after iron ion decomplexation by the siderophore compared to the signal without DFOA (SNR = 13.0; Fig. 2c). Signal restoration was also observed in 31P-MRI, with the DFOA-added probe recording an increase in signal intensity (SNR = 3.0 vs 4.3). As shown in the sensitivity curve for the relative 31P-MRI signal intensity (Fig. 3b), the addition of DFOA increased signal detectability by 26 %.
The 31P T1 relaxation times experiment required a long acquisition time (up to 150 min) due to low phosphorus sensitivity, with the sedimentation of nanoparticles affecting measurement results. Nonetheless, 31P relaxometry confirmed the iron effect, where the relaxation time decreased with iron doping (T1 = 1278.7 to 277.9 ms; 0 to 0.68 mmol L− 1 Fe3+) and T1 = 266.7/229.1 ms for the 2.73 mmol L− 1 Fe3+ with or without DFOA. The 31P T2 relaxation time for the iron-doped probe was also shorter (2.73 mmol L− 1 Fe3+; T2 = 19.1 ms) than for the probe without iron (T2 = 121.3 ms). Results are summarised in Fig. 2, Table 1 and Table 2.
|
Fe [mmol L− 1] |
SNR from 1H MRI |
SNR from 31P MRI |
SNR from 31P MRS |
|||
|---|---|---|---|---|---|---|
|
0 |
86.2 |
41.8 |
45.9 |
|||
|
0.68 |
50.1 |
4.8 |
NM* |
|||
|
2.73 |
25.4 |
3.0 |
13.0 |
|||
|
5.43 |
25.9 |
NA* |
NA* |
|||
|
13.6 |
1.2 |
NA* |
NA* |
|||
|
2.73 + DFOA |
34.7 |
4.3 |
72.2 |
|||
|
Fe [mmol L− 1] |
31P T1 [ms] |
1H T1 [ms] |
31P T2 [ms] |
1H T2 [ms] |
|---|---|---|---|---|
|
0 |
1278.7 |
122.3 |
121.3 |
33.9 |
|
0.68 |
277.9 |
111.7 |
NM* |
26.4 |
|
2.73 |
266.7 |
101.3 |
19.1 |
22.2 |
|
5.43 |
NA* |
88.0 |
NM* |
19.9 |
|
13.6 |
NA* |
69.3 |
NM* |
18.7 |
| *NA – not applicable; NM – not measured | ||||
Cell viability assay
Based on alamarBlue Assay, the 2.04 mmol L− 1 Fe3+ probe did not significantly influence cell viability up to 0.44 mg mL− 1 ([2.04 mmol L− 1 Fe3+]:[cultivation medium] ratio 1:1). The level of absorbance at 570 nm reflected the level of cell proliferation. Compared to control cells, Caco-2 incubated with a 0.22 mg mL− 1 solution of the probe in growth medium resulted in a 103 % reduction of resazurin after 24 hours. Under the same conditions, HepG2 cells attained a 105.6 % reduction, demonstrating low toxicity at the given concentration. It should be noted that even low concentrations are suitable for MR analysis, decreasing any potential toxic influence of iron ions. Results are summarised in Fig. 4.
Acquisition of a reproducible and quantifiable MR signal requires a homogeneous magnetic field throughout the sample. The custom coil presented in this article, which is designed for use in MR experiments, meets these homogeneity requirements, generating limited signal attenuation (∆5 dB) and high sensitivity for such a small volume of interest. Moreover, we assume the signal can be further amplified to improve the SNR using 1H RF part for nuclear Overhauser enhancement and 31P-1H decoupling or the quadrature 31P circular polarisation system. From our previous experience, we can say that the tested solenoid coil provided up to 40 times higher sensitivity than commercial surface coils at a diameter of 40 mm. We found that with increasing iron concentrations in the probe, both 1H and 31P relaxation times shortened. However, our 31P-MR results produced inconsistencies, with signals proving insufficient for quantification possibly due to lower calcium phytate nanoparticle colloidal stability. This can be accompanied by sedimentation during long measurements or low SNR and sensitivity at the 4.7 T magnetic field strength. Nonetheless, the adequate iron/phosphorus concentration achieved would make it suitable for use even at the low magnetic fields used in clinical practice. With regard to hydrogen relaxation times, short-time acquisitions served to decrease the influence of sedimentation. High concentrations of phytate may also have impacted on quantification, with relaxation times reducing even in the absence of iron. This may in turn have affected the phosphorus T1 relaxation time recorded for the 2.73 mmol L− 1 Fe3+ probe after chelation, which was slightly shorter after the addition of DFOA (data not shown). However, SNR after chelation increased in respect of both 1H/31P imaging and 31P spectroscopy.
We found that iron had a sufficient influence on 1H-MR contrast, rendering the probe suitable for use at low iron concentrations similar to those used for clinically approved contrast agents, e.g. Resovist with cFe = 0.45–0.7 mmol L− 1 35. As the iron molar fraction primary changed the T2 relaxation time of the probe (r2/r1 = 2.45), one can assume it acts as a T2 contrast agent. Signal detection decreased in correlation with increased iron concentration for both 1H and 31P MR. The probes therefore possess double contrast capability, influencing both 1H and 31P-MR modalities in a similar way. Our data indicate that samples with high iron content would be less practical, as high concentrations of ferric ions can saturate siderophores’ binding capacity or prolong iron decomplexation, which will result in extended experiment time and lower sensitivity. Therefore, we concluded that the sample with lower iron content, although sufficient for signal broadening, would benefit from further experiments and signal restoration. To test signal restoration under conditions mimicking bacterial infection, DFOA was added to the phytate probe containing 2.73 mmol L− 1 Fe3+. Even though the DFOA/Fe3+ complex signal did not reach the value of the iron-free probe during the experiment, signal restoration was visible in MR results. Using both modalities, the sample was adept at observing signal changes in dual 1H/31P contrasts. Additionally, complexed iron was distant enough from the phosphorus atoms, in agreement with previous results by Pechrova et al16. Accordingly, Fe3+ released from the nanoparticle and complexed with DFOA had a limited effect on the 31P-MR signal, despite slightly reducing relaxation times. The DFOA siderophore, a potent chelator of iron ions, proved sufficient at low iron concentrations. Phytate is also known to chelate iron36 by lowering its absorption in the gastrointestinal tract, even though gastric acid lowers pH in the proximal duodenum and enhances the solubility and uptake of ferric iron, and transferrin with ferritin bind circulating plasma iron37. This competition can be mitigated by changing phytate/Fe3+ concentrations depending on the injection site, given that siderophores are secreted under iron-stress conditions in gut microbiota. Furthermore, phosphorus imaging in the bacterial-overload can be used to track iron chalation rate in antibiotic-siderophore conjugates, which are more susceptible in different bacteria strains than those with the unlinked drugs34. The nanometer-sized range and amorphous nature of the particles accelerate iron complexation by siderophores. When targeting the probe, the background of the naturally occurring 31P-MR signal from the organism should also be considered.
Our cytotoxicity assay results confirm that our novel probe exhibits low toxicity, an effect possibly triggered by the presence of iron. In addition, it has been demonstrated that the amount of iron can be adjusted, with probes containing low quantities of Fe3+ ions also providing sufficient contrast.
We present one of the first phosphorus-based responsive contrast agents for 1H and 31P magnetic resonance. In our study, MR relaxometry, imaging and spectroscopy all confirmed the probe to be of sufficient sensitivity at a magnetic field similar to that used in clinical practice. In both spectroscopic and imaging experiments, the probe proved responsive to iron chelation with deferroxamine, which mimicked the presence of bacterial siderophores. It can therefore be assumed that 31P MR signal changes could serve as a marker in reflecting the presence of pathogens. Exhibiting low cytotoxicity, this phosphorus-based contrast agent is also suitable for in vivo use.
MR experiments using calcium phytate phantoms were performed on the Bruker Biospec 47/20 4.7 T MR experimental scanner (Bruker Biospec, Ettlingen, Germany) and the 1.5 T Minispec 60 MHz relaxometer (Bruker Biospin, Ettlingen, Germany). MR properties of nanoparticles were determined using MRI, MRS and relaxometry. The chemical and NMR (Bruker Avance DPX 300 NMR) properties of the probe have been previously described (see16).
Calcium phytate nanoparticles
Nanoparticles are based on calcium phytate (CaIP6) with a phosphorus concentration (cP) of 1 mmol L−1 and a nanoparticle concentration (cn) of 0.89 mg mL−1 doped with different concentrations of paramagnetic Fe3+ ions (cFe = 0, 0.68, 2.04, 2.73, 5.43, 13.6 mmol L−1) coordinated to the phytate anion by partial replacement of Ca2+ ions, as previously described16. Calcium phytate nanoparticles were synthetised by mixing an aqueous solution of calcium nitrate (c = 1 M; adjusted by 1 M NaOH to a final pH of 8) with an equal volume of an aqueous solution of sodium phytate (c = 0.16 M, pH 8). Doping of calcium phytate nanoparticles with iron ions was achieved by mixing an aqueous solution of calcium nitrate (c = 1 M) with an aqueous solution of FeCl3 (c = 1 M) at a different ratio prior to adding the IP6 solution; otherwise, the preparation procedure was the same as mentioned above. Hydrodynamic diameters dN of neat CaIP6 particles was slightly above 100 nm with the highest value of 439 nm for 5.43 mM Fe3+ dopant16. Simulation of Fe3+ release was performed by incubating CaIP6/Fe3+ nanoparticles with DFOA ([DFOA]:[CaIP6 with 2.73 mM Fe3+] ratio 1:1; 2 hours incubation), a bacterial siderophore with high affinity for Fe3+ ions. For testing, a 2.73 mmol L−1 Fe3+ probe was used. A sufficient iron concentration was generated for phosphorus MR signal broadening (see 3.3.) while avoiding over-chelation. The integrity of calcium phytate nanoparticles after the complexation of iron was proven (number average diameter of calcium phytate nanoparticles measured before addition of DFOA and 2 hours after addition was 272 ± 41 nm and 288 ± 59 nm, respectively16).
Phantoms for MR imaging and spectroscopy were tested at all iron concentrations and prepared in 500-µl Eppendorf tubes (Eppendorf AG, Germany) and in NMR tubes for 1H relaxometry. Due to its tendency for sedimentation, calcium phytate was mixed repetitively using a vortex mixer before all measurements.
1H/31P magnetic resonance radiofrequency coil
The MR signal was acquired using a custom-made dual 1H/31P RF 4-turn one-channel solenoid coil (diameter = 8 mm, height = 8 mm). The volume of interest in the coil cavity was 400 µl, with the coil structure designed for 500-µl tubes. The coil was run at 200 and 81 MHz frequencies (1H and 31P, respectively), with retuning performed on a rotating capacitive trimmer (2–64 pF). A matching capacitive trimmer (4–16 pF) was incorporated to suppress waves reflected in the cable, which can cause signal attenuation. Coil homogeneity was measured on a water phantom (500-µl tube filled with H2O) by 1H-MRI, assuming homogeneity of the phosphorus signal was comparable. For homogeneity assessment, we used a gradient echo with a low flip-angle (FLASH) sequence (repetition time/echo time TR/TE = 111.7/3.7 ms, 10° flip angle, 256×256 matrix, field of view FOV = 40×40 mm, slice thickness = 2 mm, scan time ST = 28 s).
Magnetic resonance relaxometry
Hydrogen T1 and T2 relaxation times were evaluated using phantoms with different iron concentrations (V = 240 µl; cP/cFe = 1/0–13.6 mmol L−1) measured at a stable 37 °C temperature. All data points were measured as the average of three repetitions. An inversion recovery sequence (TR = 0.01–10 000 ms, TE = 0.05 ms, recycle delay = 2 s, 1 scan, 8 points for fitting) was used to measure T1 relaxation times. T2 relaxation times were measured using the Carr-Purcell-Meiboom-Gill sequence (TR/TE = 10 000/0.05 ms, recycle delay = 2 s, 8 scans, 20 000 points for fitting). Low scan numbers and data points, which resulted in short acquisition times, were applied to eliminate the influence of sedimentation. 1H T1 and T2 relaxation times were fitted automatically using Minispec software.
Phosphorus T1 relaxation times (V = 500 µl; cP/cFe = 1/0–13.6 mmol L−1) were measured based on 10 spectroscopic single-pulse sequences with varying repetition times (TR = 200–3000 ms, ST = 10–150 min). 31P T2 relaxation times (V = 500 µl; probe without iron and with 2.73 mmol L−1 Fe3+) were measured based on 10 spectroscopic Carr-Purcell-Meiboom-Gill (CPMG) sequences with varying echo times (TR = 5000 ms, TE = 2–1200 ms, ST ~ 90 min).
Magnetic resonance imaging and spectroscopy
Hydrogen MR imaging (FLASH sequence; TR/TE = 100/6 ms) was first applied for phantom positioning. All phantoms (V = 500 µl; cP = 1 mmol L−1) were measured at different Fe3+ concentrations (cFe = 0–13.6 mmol L−1) as part of one measurement using a custom-made holder and surface 1H/31P coil (area of interest 40×40 mm). To visualise the influence of iron on the 1H MR signal, T2-weighted 1H-MRI (Rapid Acquisition with Relaxation Enhancement RARE sequence; TR/TE = 2000/24 ms; scan time ST = 6 min 24 s, spatial resolution 0.25×0.25×1.5 mm) was used.
Phosphorus MR imaging of phantoms (V = 500 µl; cP/cFe = 1/0–13.6 mmol L−1) was carried out using MR spectroscopic imaging (MRSI chemical shift imaging CSI sequence; TR/TE = 500/15 ms, ST = 60 min, spatial resolution 2.5×2.5×5.8 mm). A non-localised single-pulse sequence was used to obtain 31P spectra of the probe chelated with DFOA (before/after chelation; TR = 500 ms, ST = 16 h 40 min).
Magnetic resonance data quantification
MR imaging and spectroscopy data were quantified by calculating the SNR in order to determine the influence of iron on the MR signal. 1H and 31P-MRI signal intensities of phantoms were acquired by manual segmentation using ImageJ software (National Institutes of Health, Bethesda, USA). The SNR was calculated as a ratio of the signal intensity and standard deviation of the surrounding noise (Equation 1):
(1)
where S is the signal intensity in the region of interest (ROI), σ is the standard deviation of background noise, with constant 0.655 reflecting the Rician distribution of background noise in the magnitude MR image38.
For 31P MR spectra, SNR quantification and subsequent calculation of T1/T2 relaxation times, a signal integral value was used. Phosphorus spectroscopic data were processed using Matlab software (Matlab R2007b, The MathWorks Inc., USA) script. Topspin software (Bruker, ParaVision 4) was used for 31P signal integral and noise calculation. The evaluation consisted of the following steps: the first was to mark the 31P peak to determine the integral of evaluation areas (∑Isignal); the second was to select the noise region of the spectrum that had shifted sufficiently (∆7 ppm) from the peak; and the third was to calculate the noise integral value (∑Inoise). The SNR was calculated from the resulting values of the signal integral divided by the noise integral (Equation 2):
(2)
Finally, for 31P T1 and T2relaxation times measurement, integrals from the evaluation area were fitted on exponential curves to the repetition time/echo time, respectively. ImageJ software was used for curve fitting followed by calculation of relaxation times. Signal intensity dependence on iron concentration and chelation was plotted to determine the effect of iron and DFOA on signal intensity changes for both 1H and 31P MRI.
Cytotoxicity assay
Probe cytotoxicity was tested on the human hepatoma cell line (HepG2) (a standard in vitro testing model) and the human colorectal adenocarcinoma cell line (CaCo-2) (replicating the gastrointestinal tract environment in which iron complexation can occur) to establish viability of the cells for use in in vivo experiments. The assay was tested using a 2.04 mmol L−1 Fe3+ probe – at a sufficient iron concentration for MR studies while ensuring a low iron impact on cell viability – at three concentrations of 0.22–0.89 mg mL−1.
Cells were cultured in a standard humidified atmosphere containing 5 % CO2 and 37 °C. CaCo-2 cells were grown in full high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, USA) supplemented with 4.5 g L−1 D-glucose, 10 % foetal bovine serum, 0.5 % penicillin/streptomycin and 4 mmol L−1 L-glutamine. For HepG2 cells, a full Roswell Park Memorial Institute medium (RPMI, Thermo Fisher Scientific, USA 10 % fetal bovine serum, 1 % penicillin/streptomycin, 2 mmol L−1 L-glutamine) was prepared. alamarBlue Cell Viability Reagent (Thermo Fisher Scientific, USA) is a resazurin-based solution that functions as a cell health indicator and is used to reduce the power of living cells and quantitatively measure viability. Cells were seeded in 96-well plates at a density of 1x104 cells/well, counted using the Vi-CELL™XR Cell Viability Analyzer (Beckman Coulter, USA) and left to incubate for 24 hours (HepG2) and 48 hours (Caco-2). The phytate calcium probe was filtrated using a 0.2-μm filter, diluted in the fresh growth medium and added to the wells (100 μl/well). The effect of the contrast agent on cell viability was assessed based on 24-hour incubation following 3-hour (HepG2 cells) and 6-hour (CaCo-2 cells) incubations with 10 % alamarBlue. Resazurin, the active component of the reagent, was reduced to resorufin only in viable cells; absorbance in the test wells was detected at 570/600 nm using the Elisa Microplate Reader RT-6900. The percentage of alamarBlue reduction was then calculated as a percentage of the control cell reduction. The assay was conducted in triplicate.
Data availability
All relevant data are included in the manuscript. Used datasets are available from the corresponding author on reasonable request.
Acknowledgements
This study was supported by the Charles University Grant Agency, GA UK grant number 358119; Charles University, First Faculty of Medicine; the Ministry of Health of the Czech Republic (grant number NU20-08-00095); and the Institute for Clinical and Experimental Medicine IKEM, IN00023001.
Author contributions
N.Z. designed the experiment, collected and analysed the data, was responsible for the data interpretation and wrote the manuscript, M.V. collected and analysed the data, designed new hardware, R.L. contributed in the data interpretation and writing of the manuscript, D.J. designed and supervised the project, was responsible for the data interpretation and integrity of this work. All authors reviewed the manuscript and approved the final version.
Competing interests
The authors declare no competing interests.
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No competing interests reported.