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Research
Susheng Miao
Harbin Medical University Third Hospital: Tumor Hospital of Harbin Medical University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
In the past few years, immunotherapy has changed the way we treat solid tumors. People pay more and more attention to the immune microenvironment of laryngeal squamous cell carcinoma (LSCC). In this study, our immunotherapy research took advantage of the clinical database and focused our in-depth analysis on the tumor microenvironment (TME).
Methods
This study evaluated the relationship between the clinical outcome and the local tissue and overall immune status in 412 patients with primary LSCC. We constructed and validated a risk model that could predict prognosis, assess immune status, identify high-risk patients, and develop personalized treatment plans through bioinformatics. In addition, through immunohistochemical analysis, we verified the differential expression of CTSL and KDM5D genes with the largest weight coefficients in the model in LSCC tissues and their influence on the prognosis and tumor-infiltrating lymphocytes (TILs).
Results
We found that interstitial tumor-infiltrating lymphocytes (iTILs), tumor parenchymal infiltrating lymphocyte volume (TILv), tumor infiltrates lymphocytes of frontier invasion (TILf), and the platelet-to lymphocyte ratio (PLR) were independent factors affecting the prognosis of patients with LSCC. A novel risk model can guide clinicians to accurately predict prognosis, identify high-risk patients, and formulate personalized treatment plans. The differential expression of genes such as CTSL and KDM5D can significantly affect the TILs of LSCC and the prognosis of patients.
Conclusion
Local and systemic inflammatory markers in patients with laryngeal squamous cell carcinoma are reliable prognostic factors. The risk model and CTSL, KDM5D gene have important potential research value.
Keywords
Laryngeal squamous cell carcinoma (LSCC), Intratumoral infiltrating lymphocytes (iTILs), infiltrating lymphocyte volume (TILv), Tumor infiltrates lymphocytes of frontier invasion (TILf), overall survival (OS), tumor microenvironment (TME), The Cancer Genome Atlas (TCGA), Tumor mutation burden (TMB), immunotherapy
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This is a preprint. It has not completed peer review.
Research
Susheng Miao
Harbin Medical University Third Hospital: Tumor Hospital of Harbin Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 07 Jul, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cancer Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
In the past few years, immunotherapy has changed the way we treat solid tumors. People pay more and more attention to the immune microenvironment of laryngeal squamous cell carcinoma (LSCC). In this study, our immunotherapy research took advantage of the clinical database and focused our in-depth analysis on the tumor microenvironment (TME).
Methods
This study evaluated the relationship between the clinical outcome and the local tissue and overall immune status in 412 patients with primary LSCC. We constructed and validated a risk model that could predict prognosis, assess immune status, identify high-risk patients, and develop personalized treatment plans through bioinformatics. In addition, through immunohistochemical analysis, we verified the differential expression of CTSL and KDM5D genes with the largest weight coefficients in the model in LSCC tissues and their influence on the prognosis and tumor-infiltrating lymphocytes (TILs).
Results
We found that interstitial tumor-infiltrating lymphocytes (iTILs), tumor parenchymal infiltrating lymphocyte volume (TILv), tumor infiltrates lymphocytes of frontier invasion (TILf), and the platelet-to lymphocyte ratio (PLR) were independent factors affecting the prognosis of patients with LSCC. A novel risk model can guide clinicians to accurately predict prognosis, identify high-risk patients, and formulate personalized treatment plans. The differential expression of genes such as CTSL and KDM5D can significantly affect the TILs of LSCC and the prognosis of patients.
Conclusion
Local and systemic inflammatory markers in patients with laryngeal squamous cell carcinoma are reliable prognostic factors. The risk model and CTSL, KDM5D gene have important potential research value.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
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