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Research Article
Christiane Abreu Paiva
Embrapa Maize and Sorghum: Embrapa Milho e Sorgo
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1874-2489
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This work is licensed under a CC BY 4.0 License. Read Full License
Usage of Bacillus and Azospirillum as new eco-friendly microbial consortium inoculants is a strategy to increase plant growth and crop yield by improving nutrient availability in agricultural systems. In this study, we designed a multispecies inoculum containing B. thuringiensis (strain B116), B. subtillis (strain B2084) and Azospirillum brasilense (strains A1626 and A2142) to investigate their individual or co-inoculated ability to solubilize and mineralize phosphate, produce indole acetic acid (IAA) and their effect on maize growth promotion in hydroponics and in a non-sterile soil. All strains showed significant IAA production, P mineralization (sodium phytate) and Ca-P, Fe-P (tricalcium phosphate and iron phosphate, respectively) solubilization. In hydroponics, co-inoculation with A1626 x A2142, B2084 x A2142, B2084 x A1626 resulted in higher root total length, total surface area, and surface area of roots with diameter between 0 and 1 mm than other treatments with single inoculant, except B2084. In a greenhouse experiment, maize inoculated with the two Azospirillum strains exhibited enhanced shoot dry weight, shoot P and K content, root dry weight, root N and K content and acid and alkaline phosphatase activities than the other treatments. There was a significant correlation between soil P and P shoot, alkaline phosphatase and P shoot and between acid phosphatase and root dry weight. It may be concluded that co-inoculations are most effective than single inoculants strains, mainly between two selected Azospirillum strains. Thus, they could have synergistic interactions during maize growth, and be useful in the formulation of inoculants to improve the cropping systems sustainable.
Keywords
Zea mays L, plant growth-promoting bacteria (PGPB), combined microbial inoculants, dual inoculation
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CURRENT STATUS: Under Review
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Posted 09 Jul, 2021
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Received 21 Jul, 2021
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This preprint is under consideration at Archives of Microbiology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Christiane Abreu Paiva
Embrapa Maize and Sorghum: Embrapa Milho e Sorgo
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1874-2489
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 09 Jul, 2021
No community comments so far
Reviews received
Received 21 Jul, 2021
Reviewers invited
Invitations sent on 06 Jul, 2021
Editor assigned
On 05 Jul, 2021
First submitted
On 30 Jun, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Usage of Bacillus and Azospirillum as new eco-friendly microbial consortium inoculants is a strategy to increase plant growth and crop yield by improving nutrient availability in agricultural systems. In this study, we designed a multispecies inoculum containing B. thuringiensis (strain B116), B. subtillis (strain B2084) and Azospirillum brasilense (strains A1626 and A2142) to investigate their individual or co-inoculated ability to solubilize and mineralize phosphate, produce indole acetic acid (IAA) and their effect on maize growth promotion in hydroponics and in a non-sterile soil. All strains showed significant IAA production, P mineralization (sodium phytate) and Ca-P, Fe-P (tricalcium phosphate and iron phosphate, respectively) solubilization. In hydroponics, co-inoculation with A1626 x A2142, B2084 x A2142, B2084 x A1626 resulted in higher root total length, total surface area, and surface area of roots with diameter between 0 and 1 mm than other treatments with single inoculant, except B2084. In a greenhouse experiment, maize inoculated with the two Azospirillum strains exhibited enhanced shoot dry weight, shoot P and K content, root dry weight, root N and K content and acid and alkaline phosphatase activities than the other treatments. There was a significant correlation between soil P and P shoot, alkaline phosphatase and P shoot and between acid phosphatase and root dry weight. It may be concluded that co-inoculations are most effective than single inoculants strains, mainly between two selected Azospirillum strains. Thus, they could have synergistic interactions during maize growth, and be useful in the formulation of inoculants to improve the cropping systems sustainable.
Figure 1
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