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Transcriptome Analysis Reveals Key Genes Involved in the Regulation of Nicotine Biosynthesis at Early Time Points after Topping in Tobacco (Nicotiana tabacum L.)
Guiling Sun
Henan University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7580-8301
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Background： Nicotiana tabacum is an important economic crop. Topping, a common agricultural practice employed with flue-cured tobacco, is designed to increase leaf nicotine contents. Many genes are found to be differentially expressed in response to topping, particularly genes involved in nicotine biosynthesis, but comprehensive analyses of early transcriptional responses induced by topping are not yet available. To develop a detailed understanding of the mechanisms regulating nicotine biosynthesis after topping, we have sequenced the transcriptomes of N. tabacum roots at seven time points following topping.
Results： Differential expression analysis revealed that 4,830 genes respond to topping across all time points. Amongst these, nine gene families involved in nicotine biosynthesis and two gene families involved in nicotine transport showed significant changes during the immediate 24 hour period following topping. No obvious preference to the parental species was detected in the differentially expressed genes (DEGs). Significant changes in transcript levels of nine genes involved in nicotine biosynthesis and phytohormone signal transduction were validated by qRT-PCR assays. 549 genes encoding transcription factors (TFs), found to exhibit significant changes in gene expression after topping, formed 15 clusters based on similarities of their transcript level time-course profiles. 336 DEGs involved in phytohormone signal transduction, including genes functionally related to the phytohormones jasmonic acid, abscisic acid, auxin, ethylene, and gibberellin, were identified at the earliest time point after topping.
Conclusions： Our research provides the first detailed analysis of the early transcriptional responses to topping in N. tabacum , and identifies excellent candidates for further detailed studies concerning the regulation of nicotine biosynthesis in tobacco roots.
Keywords
Tobacco; topping; transcriptome analysis; differentially expressed genes; nicotine biosynthesis and regulation
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Transcriptome Analysis Reveals Key Genes Involved in the Regulation of Nicotine Biosynthesis at Early Time Points after Topping in Tobacco (Nicotiana tabacum L.)
Guiling Sun
Henan University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7580-8301
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 31 Dec, 2019
Published
On 20 Jan, 2020
No community comments so far
Editor assigned
On 27 Dec, 2019
Submission checks complete
On 26 Dec, 2019
Editor invited
On 26 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 20 Dec, 2019
Review #1 received
Received 13 Dec, 2019
Reviewer #1 agreed
On 28 Oct, 2019
Reviewers invited
Invitations sent on 21 Oct, 2019
Submission checks complete
On 10 Oct, 2019
Editor assigned
On 07 Oct, 2019
Editor invited
On 06 Oct, 2019
First submitted
On 02 Oct, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Background： Nicotiana tabacum is an important economic crop. Topping, a common agricultural practice employed with flue-cured tobacco, is designed to increase leaf nicotine contents. Many genes are found to be differentially expressed in response to topping, particularly genes involved in nicotine biosynthesis, but comprehensive analyses of early transcriptional responses induced by topping are not yet available. To develop a detailed understanding of the mechanisms regulating nicotine biosynthesis after topping, we have sequenced the transcriptomes of N. tabacum roots at seven time points following topping.
Results： Differential expression analysis revealed that 4,830 genes respond to topping across all time points. Amongst these, nine gene families involved in nicotine biosynthesis and two gene families involved in nicotine transport showed significant changes during the immediate 24 hour period following topping. No obvious preference to the parental species was detected in the differentially expressed genes (DEGs). Significant changes in transcript levels of nine genes involved in nicotine biosynthesis and phytohormone signal transduction were validated by qRT-PCR assays. 549 genes encoding transcription factors (TFs), found to exhibit significant changes in gene expression after topping, formed 15 clusters based on similarities of their transcript level time-course profiles. 336 DEGs involved in phytohormone signal transduction, including genes functionally related to the phytohormones jasmonic acid, abscisic acid, auxin, ethylene, and gibberellin, were identified at the earliest time point after topping.
Conclusions： Our research provides the first detailed analysis of the early transcriptional responses to topping in N. tabacum , and identifies excellent candidates for further detailed studies concerning the regulation of nicotine biosynthesis in tobacco roots.
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Figure 2
Figure 3
Figure 4
Figure 5
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