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Nathalia Bibiana Teixeira
Universidade Estadual Paulista Julio de Mesquita Filho Instituto de Biociencias Campus de Botucatu
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6185-6086
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Individuals with insulin-dependent diabetes are a risk group for infections caused by Staphylococcus aureus. The objective of this study was to determine the prevalence and risk factors for nasal and oropharyngeal carriage of S. aureus and MRSA in insulin-dependent diabetic individuals from Botucatu, São Paulo, Brazil. Additionally, susceptibility profiling, detection of the mecA gene, SCCmec typing and molecular typing by PFGE and MLST were performed in nasal and oropharyngeal swabs of 312 subjects. The prevalence of S. aureus and MRSA was 30.4% and 4.8%, respectively. SCCmec type IV was the predominant type among isolates, although some carried SCCmec types I and II. MRSA and MSSA clones were detected among the isolates of this population. In addition, an important clonal lineage (ST398) was identified among resistant and susceptible isolates. Age and lung disease were negatively associated with S. aureus carriage, while lower-extremity ulcers were a risk factor for S. aureus carriage. For MRSA, only male gender was a risk factor. These data suggest widespread dissemination of MRSA in the insulin-dependent diabetic population studied, as well as the emergence of important lineages among these individuals.
Keywords
methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), diabetes mellitus, insulin, resistance, molecular typing
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CURRENT STATUS: Published
View the published article at Annals of Clinical Microbiology and Antimicrobials.
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Editorial decision: Accept
On 12 Nov, 2020
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On 12 Nov, 2020
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On 12 Nov, 2020
Editor invited
On 12 Nov, 2020
Version 1
Posted 02 Sep, 2020
No comments provided
Editorial decision: Major revision
On 01 Oct, 2020
Review #6 received
Received 30 Sep, 2020
Review #3 received
Received 20 Sep, 2020
Review #4 received
Received 19 Sep, 2020
Review #5 received
Received 19 Sep, 2020
Review #2 received
Received 15 Sep, 2020
Review #1 received
Received 14 Sep, 2020
Reviewer #6 agreed
On 13 Sep, 2020
Reviewer #5 agreed
On 12 Sep, 2020
Reviewer #4 agreed
On 11 Sep, 2020
Reviewer #2 agreed
On 10 Sep, 2020
Reviewer #3 agreed
On 10 Sep, 2020
Reviewers invited
Invitations sent on 09 Sep, 2020
Reviewer #1 agreed
On 09 Sep, 2020
Editor assigned
On 07 Sep, 2020
Editor invited
On 06 Sep, 2020
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On 31 Aug, 2020
First submitted
On 27 Aug, 2020
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Subject Areas
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See the published version of this preprint at Annals of Clinical Microbiology and Antimicrobials.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Nathalia Bibiana Teixeira
Universidade Estadual Paulista Julio de Mesquita Filho Instituto de Biociencias Campus de Botucatu
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6185-6086
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Annals of Clinical Microbiology and Antimicrobials.
No community comments so far
Editorial decision: Accept
On 12 Nov, 2020
Editor assigned
On 12 Nov, 2020
Submission checks complete
On 12 Nov, 2020
Editor invited
On 12 Nov, 2020
Version 1
Posted 02 Sep, 2020
No comments provided
Editorial decision: Major revision
On 01 Oct, 2020
Review #6 received
Received 30 Sep, 2020
Review #3 received
Received 20 Sep, 2020
Review #4 received
Received 19 Sep, 2020
Review #5 received
Received 19 Sep, 2020
Review #2 received
Received 15 Sep, 2020
Review #1 received
Received 14 Sep, 2020
Reviewer #6 agreed
On 13 Sep, 2020
Reviewer #5 agreed
On 12 Sep, 2020
Reviewer #4 agreed
On 11 Sep, 2020
Reviewer #2 agreed
On 10 Sep, 2020
Reviewer #3 agreed
On 10 Sep, 2020
Reviewers invited
Invitations sent on 09 Sep, 2020
Reviewer #1 agreed
On 09 Sep, 2020
Editor assigned
On 07 Sep, 2020
Editor invited
On 06 Sep, 2020
Submission checks complete
On 31 Aug, 2020
First submitted
On 27 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Annals of Clinical Microbiology and Antimicrobials.
Individuals with insulin-dependent diabetes are a risk group for infections caused by Staphylococcus aureus. The objective of this study was to determine the prevalence and risk factors for nasal and oropharyngeal carriage of S. aureus and MRSA in insulin-dependent diabetic individuals from Botucatu, São Paulo, Brazil. Additionally, susceptibility profiling, detection of the mecA gene, SCCmec typing and molecular typing by PFGE and MLST were performed in nasal and oropharyngeal swabs of 312 subjects. The prevalence of S. aureus and MRSA was 30.4% and 4.8%, respectively. SCCmec type IV was the predominant type among isolates, although some carried SCCmec types I and II. MRSA and MSSA clones were detected among the isolates of this population. In addition, an important clonal lineage (ST398) was identified among resistant and susceptible isolates. Age and lung disease were negatively associated with S. aureus carriage, while lower-extremity ulcers were a risk factor for S. aureus carriage. For MRSA, only male gender was a risk factor. These data suggest widespread dissemination of MRSA in the insulin-dependent diabetic population studied, as well as the emergence of important lineages among these individuals.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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