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Research article
Emilie Vangrinsven
Universite de Liege
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8663-9275
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This work is licensed under a CC BY 4.0 License. Read Full License
Background – Extrinsic and intrinsic factors have been shown to influence nasal microbiota (NM) in humans. Very few studies investigated the association between nasal microbiota and factors like facial/body conformation, age, and environment in dogs. Objectives are to investigate variations in NM in healthy dogs with different facial and body conformations and to assess the influence of age and living environment. A total of 46 dogs of different age, living environment and from 3 different breed groups were recruited: 22 meso-/dolichocephalic medium to large breed dogs, 12 brachycephalic dogs and 12 terrier breeds. The nasal bacterial microbiota was assessed through sequencing of 16S rRNA gene (V1-V3 regions) amplicons.
Results – We showed major differences in the NM composition together with increased richness and α-diversity in brachycephalic dogs, compared to meso-/dolichocephalic dogs and dogs from terrier breeds. We failed to detect any effect of age or environment.
Conclusion – Healthy brachycephalic breeds and their unique facial conformation is associated with a distinct NM profile. Description of the NM in healthy dogs serves as a foundation for future researches assessing the changes associated with disease and the modulation of NM communities as a potential treatment.
Keywords
brachycephalic breeds, influence, facial/body, influence nasal microbiota
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CURRENT STATUS: Under Review
Version 1
Posted 08 Sep, 2020
No community comments so far
Editorial decision: Major revision
On 04 Apr, 2021
Review #2 received
Received 29 Mar, 2021
Reviewer #2 agreed
On 15 Mar, 2021
Review #1 received
Received 10 Nov, 2020
Reviewer #1 agreed
On 14 Sep, 2020
Editor assigned
On 04 Sep, 2020
Reviewers invited
Invitations sent on 04 Sep, 2020
Submission checks complete
On 03 Sep, 2020
Editor invited
On 03 Sep, 2020
First submitted
On 30 Aug, 2020
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Subject Areas
Small Animal Medicine
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A new preprint design is coming!
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This preprint is under consideration at BMC Veterinary Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Emilie Vangrinsven
Universite de Liege
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8663-9275
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 08 Sep, 2020
No community comments so far
Editorial decision: Major revision
On 04 Apr, 2021
Review #2 received
Received 29 Mar, 2021
Reviewer #2 agreed
On 15 Mar, 2021
Review #1 received
Received 10 Nov, 2020
Reviewer #1 agreed
On 14 Sep, 2020
Editor assigned
On 04 Sep, 2020
Reviewers invited
Invitations sent on 04 Sep, 2020
Submission checks complete
On 03 Sep, 2020
Editor invited
On 03 Sep, 2020
First submitted
On 30 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Small Animal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background – Extrinsic and intrinsic factors have been shown to influence nasal microbiota (NM) in humans. Very few studies investigated the association between nasal microbiota and factors like facial/body conformation, age, and environment in dogs. Objectives are to investigate variations in NM in healthy dogs with different facial and body conformations and to assess the influence of age and living environment. A total of 46 dogs of different age, living environment and from 3 different breed groups were recruited: 22 meso-/dolichocephalic medium to large breed dogs, 12 brachycephalic dogs and 12 terrier breeds. The nasal bacterial microbiota was assessed through sequencing of 16S rRNA gene (V1-V3 regions) amplicons.
Results – We showed major differences in the NM composition together with increased richness and α-diversity in brachycephalic dogs, compared to meso-/dolichocephalic dogs and dogs from terrier breeds. We failed to detect any effect of age or environment.
Conclusion – Healthy brachycephalic breeds and their unique facial conformation is associated with a distinct NM profile. Description of the NM in healthy dogs serves as a foundation for future researches assessing the changes associated with disease and the modulation of NM communities as a potential treatment.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
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