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Research
Applicability of Commercial Clinical Chemistry Test Kits For Horse Serum
Yoseph Cherinet Megerssa
Addis Ababa University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1297-5329
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Validation of a test method to determine its fitness for a particular use is critical for confirming that the test can generate accurate and precise data. Although several commercial biochemical test kits exist and validated for humans there is no specific and validated commercial clinical chemistry test kits designed for horse
Objective: The aim of this study was to validate commercial clinical chemistry test kits designed for human serum for use in horses to accurately measure total cholesterol urea and total protein in serum
Methods: This study used 25 apparently healthy adult horses. Blood samples were collected and pooled serum was prepared. Analytical validation comprises replication and recovery experiments. Total observable error (TEo%), Sigma metrics and quality goal index (QGI) was used to support the analytical validation studies.
Result: Intra- and inter-assay variability was 2.05% and 2.08%, 2.26% and 1.89%, 2.4% and 1.63%, for total cholesterol urea and total protein for respectively; recovery was 99.46%, 97.32% and 100.1% for total cholesterol urea and total protein for respectively. TEo% for the specified analytes were within the allowable error indicated ASVCP guidelines. All the three anaytes satisfied the recommended requirement (>3 sigma values). The QGI for urea, as it had below 6σ was 0.95 indicating imprecision and inaccuracy.
Conclusion: The results of the research endorse the suitability of the studied commercial clinical chemistry test kits and illustrated the acceptance criteria for serum collected from horse.
Keywords
Clinical Chemistry, Horse Serum, Validation
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This is a preprint. It has not completed peer review.
Research
Applicability of Commercial Clinical Chemistry Test Kits For Horse Serum
Yoseph Cherinet Megerssa
Addis Ababa University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1297-5329
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 18 Sep, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Cell Biology & Physiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Validation of a test method to determine its fitness for a particular use is critical for confirming that the test can generate accurate and precise data. Although several commercial biochemical test kits exist and validated for humans there is no specific and validated commercial clinical chemistry test kits designed for horse
Objective: The aim of this study was to validate commercial clinical chemistry test kits designed for human serum for use in horses to accurately measure total cholesterol urea and total protein in serum
Methods: This study used 25 apparently healthy adult horses. Blood samples were collected and pooled serum was prepared. Analytical validation comprises replication and recovery experiments. Total observable error (TEo%), Sigma metrics and quality goal index (QGI) was used to support the analytical validation studies.
Result: Intra- and inter-assay variability was 2.05% and 2.08%, 2.26% and 1.89%, 2.4% and 1.63%, for total cholesterol urea and total protein for respectively; recovery was 99.46%, 97.32% and 100.1% for total cholesterol urea and total protein for respectively. TEo% for the specified analytes were within the allowable error indicated ASVCP guidelines. All the three anaytes satisfied the recommended requirement (>3 sigma values). The QGI for urea, as it had below 6σ was 0.95 indicating imprecision and inaccuracy.
Conclusion: The results of the research endorse the suitability of the studied commercial clinical chemistry test kits and illustrated the acceptance criteria for serum collected from horse.