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Inoculation of Native Symbiotic Effective Sinorhizobium Spp. Enhanced Soybean [Glycine Max (L) Merr.] Grain Yield in Ethiopia
Diriba Temesgen Dagaga
Madda Walabu University, Department of Biology, P.O.Box 437, Bale Robe, Ethiopia.
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7034-7331
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Environmental Systems Research.
Background
Soybean [Glycine max (L) Merr.] is an annual leguminous crop serving as a source of food and feed, green manure, biodiesel and fiber It is nodulated by diverse slow growing and fast growing rhizobia belonging to the genus Bradyrhizobium and Sinorhizobium, respectively. In Ethiopia, it has been cultivated since 1950s with lower grain yield history. Yield improvement efforts have been more concentrated on agronomic studies, inoculation of exotic Bradyrhizobium japonicum including TAL379 and/or fertilizer application. The results have usually been unsatisfactory and inconsistent. This study was initiated to identify promising indigenous soybean rhizobial inoculant that can enhance yield of the crop in the country.
Methods
Native soybean rhizobia, designated GMR for Glycine max rhizobia, were trapped using soybean (cv. Ethio-Yugoslavia) from soils collected across agro-ecologies of Ethiopia. Indigenous soybean rhizobia were screened for in vitro tolerance against physico-chemical stresses, plant growth promoting (PGP) traits and symbiotic performances at greenhouse and field levels A reference B. japonicum (TAL379) was included in all experiments. A soybean plant growth promoting Achromobacter sp. was also included in field trials for co-inoculation. Quantitative data were assessed by analysis of variance (ANOVA) employing SAS computer software package version 9.3. Mean separations were undertaken using the Duncan’s Multiple Range Test at p≤0.05.
Result
GMR that produced acid and grew faster with larger colonies were identified as Sinorhizobium spp. and those which produced alkali and grew slowly with smaller colonies were identified as Bradyrhizobium spp though further genetic analysis should be performed for verification and identification of their genus and species, respectively. Two Sinorhizobium spp (GMR120C and GMR125B) profoundly nodulated different soybean cultivars under greenhouse conditions and significantly improved grain yield (p≤0.05; maximum 3.98 tons ha-1) compared to 2.41, 2.82 and 2.69 recorded as maximum grain yield (tons ha-1) for TAL379 inoculation, positive control and negative control, respectively in field trials. Higher yield was recorded when GMR125B was coinoculated with Achromobacter sp., but when GMR120C was inoculated singly. These GMR also showed efficient utilization of numerous substrates, some PGP traits and potential adaptation to various ecological stresses.
Conclusion
The two Sinorhizobium spp. (GMR120C and GMR125B) are promising soybean inoculants that can be used to enhance the productivity of the crop in the country.
Keywords
Ethiopia, native Sinorhizobium, soybean, stress tolerance, nodulation, grain yield
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Received 18 Oct, 2020
Reviewer #2 agreed
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Review #1 received
Received 03 Oct, 2020
Reviewer #1 agreed
On 21 Sep, 2020
Reviewers invited
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On 14 Sep, 2020
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This preprint is under consideration at Environmental Systems Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Inoculation of Native Symbiotic Effective Sinorhizobium Spp. Enhanced Soybean [Glycine Max (L) Merr.] Grain Yield in Ethiopia
Diriba Temesgen Dagaga
Madda Walabu University, Department of Biology, P.O.Box 437, Bale Robe, Ethiopia.
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7034-7331
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 1
Posted 17 Sep, 2020
Published
On 28 Nov, 2020
No community comments so far
Editorial decision: Major Revision
On 20 Oct, 2020
Review #2 received
Received 18 Oct, 2020
Reviewer #2 agreed
On 15 Oct, 2020
Review #1 received
Received 03 Oct, 2020
Reviewer #1 agreed
On 21 Sep, 2020
Reviewers invited
Invitations sent on 18 Sep, 2020
Editor assigned
On 15 Sep, 2020
Submission checks complete
On 14 Sep, 2020
Editor invited
On 14 Sep, 2020
First submitted
On 03 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Environmental Engineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Environmental Systems Research.
Background
Soybean [Glycine max (L) Merr.] is an annual leguminous crop serving as a source of food and feed, green manure, biodiesel and fiber It is nodulated by diverse slow growing and fast growing rhizobia belonging to the genus Bradyrhizobium and Sinorhizobium, respectively. In Ethiopia, it has been cultivated since 1950s with lower grain yield history. Yield improvement efforts have been more concentrated on agronomic studies, inoculation of exotic Bradyrhizobium japonicum including TAL379 and/or fertilizer application. The results have usually been unsatisfactory and inconsistent. This study was initiated to identify promising indigenous soybean rhizobial inoculant that can enhance yield of the crop in the country.
Methods
Native soybean rhizobia, designated GMR for Glycine max rhizobia, were trapped using soybean (cv. Ethio-Yugoslavia) from soils collected across agro-ecologies of Ethiopia. Indigenous soybean rhizobia were screened for in vitro tolerance against physico-chemical stresses, plant growth promoting (PGP) traits and symbiotic performances at greenhouse and field levels A reference B. japonicum (TAL379) was included in all experiments. A soybean plant growth promoting Achromobacter sp. was also included in field trials for co-inoculation. Quantitative data were assessed by analysis of variance (ANOVA) employing SAS computer software package version 9.3. Mean separations were undertaken using the Duncan’s Multiple Range Test at p≤0.05.
Result
GMR that produced acid and grew faster with larger colonies were identified as Sinorhizobium spp. and those which produced alkali and grew slowly with smaller colonies were identified as Bradyrhizobium spp though further genetic analysis should be performed for verification and identification of their genus and species, respectively. Two Sinorhizobium spp (GMR120C and GMR125B) profoundly nodulated different soybean cultivars under greenhouse conditions and significantly improved grain yield (p≤0.05; maximum 3.98 tons ha-1) compared to 2.41, 2.82 and 2.69 recorded as maximum grain yield (tons ha-1) for TAL379 inoculation, positive control and negative control, respectively in field trials. Higher yield was recorded when GMR125B was coinoculated with Achromobacter sp., but when GMR120C was inoculated singly. These GMR also showed efficient utilization of numerous substrates, some PGP traits and potential adaptation to various ecological stresses.
Conclusion
The two Sinorhizobium spp. (GMR120C and GMR125B) are promising soybean inoculants that can be used to enhance the productivity of the crop in the country.
Figure 1