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Novel Association Between FOXO3 rs2232365 Polymorphism and Late-Onset Preeclampsia: A Case-Control Candidate Genetic Study
Ying Chen
Jilin University First Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5833-2380
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Pregnancy and Childbirth.
Background
Both genetic susceptibility and dysregulated lipid metabolism are important susceptibilities to preeclampsia. In the study, we devote to investigate the associations of FOXO3 and TLR7 genetic polymorphisms with preeclampsia in a Chinese population.
Methods
This case-control study involved 335 Han Chinese pregnant women, including 177 pregnant women with preeclampsia and 158 healthy controls. The preeclampsia group was further sub-grouped into early-onset preeclampsia (EOPE, n=70）and late-onset preeclampsia (LOPE, n=107）. Three single nucleotide polymorphisms SNPs , including FOXO3 rs2232365, rs3761548, and TLR7 rs3853839 were genotyped by multiplex PCR for targeted next-generation sequencing. The χ2 test and multiple interaction effect analyses were performed to determine the association of three SNPs with serum lipid levels and thyroid function in women with preeclampsia.
Results
Our study showed that SNP rs2232365 C allele frequencies were significantly associated with the occurrence of LOPE (P=0.022, odds ratio = 1.72 (0.95 CI: 1.23-2.41)). The genotype (CC vs TT+CT) distribution of rs2232365 revealed a significant association with LOPE (P=0.004, odds ratio = 3.497 (0.95 CI: 1.498-8.164)). No significant difference was found in the genotype and allele frequencies of rs3761548 and rs3853839 between controls and cases (P>0.05). Moreover, the genotype CC of rs2232365 was significantly correlated with increased TG/HDL levels in the LOPE group (all p=0.014).
Conclusions
The polymorphisms of rs2232365 are associated with the risk of LOPE and may modulate TG/HDL levels in pregnant women with LOPE.
Keywords
Preeclampsia, Single nucleotide polymorphism, rs2232365, BMI, lipid metabolism
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CURRENT STATUS: Published
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Posted 22 Nov, 2020
Editor assigned
On 22 Nov, 2020
Submission checks complete
On 22 Nov, 2020
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On 22 Nov, 2020
This version was not preprinted
Version (no preprint)
Posted 11 Nov, 2020
Editorial decision: Major revision
On 11 Nov, 2020
Editor assigned
On 01 Nov, 2020
Submission checks complete
On 01 Nov, 2020
Editor invited
On 01 Nov, 2020
This version was not preprinted
Version 1
Posted 17 Sep, 2020
Published
On 14 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 25 Oct, 2020
Review #2 received
Received 22 Oct, 2020
Reviewer #2 agreed
On 17 Oct, 2020
Reviewer #1 agreed
On 10 Oct, 2020
Review #1 received
Received 10 Oct, 2020
Reviewers invited
Invitations sent on 25 Sep, 2020
Editor assigned
On 08 Sep, 2020
Submission checks complete
On 07 Sep, 2020
Editor invited
On 07 Sep, 2020
First submitted
On 04 Sep, 2020
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Subject Areas
Maternal & Fetal Medicine
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This preprint is under consideration at BMC Pregnancy and Childbirth. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Novel Association Between FOXO3 rs2232365 Polymorphism and Late-Onset Preeclampsia: A Case-Control Candidate Genetic Study
Ying Chen
Jilin University First Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5833-2380
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version (no preprint)
Posted 22 Nov, 2020
Editor assigned
On 22 Nov, 2020
Submission checks complete
On 22 Nov, 2020
Editor invited
On 22 Nov, 2020
This version was not preprinted
Version (no preprint)
Posted 11 Nov, 2020
Editorial decision: Major revision
On 11 Nov, 2020
Editor assigned
On 01 Nov, 2020
Submission checks complete
On 01 Nov, 2020
Editor invited
On 01 Nov, 2020
This version was not preprinted
Version 1
Posted 17 Sep, 2020
Published
On 14 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 25 Oct, 2020
Review #2 received
Received 22 Oct, 2020
Reviewer #2 agreed
On 17 Oct, 2020
Reviewer #1 agreed
On 10 Oct, 2020
Review #1 received
Received 10 Oct, 2020
Reviewers invited
Invitations sent on 25 Sep, 2020
Editor assigned
On 08 Sep, 2020
Submission checks complete
On 07 Sep, 2020
Editor invited
On 07 Sep, 2020
First submitted
On 04 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Maternal & Fetal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Pregnancy and Childbirth.
Background
Both genetic susceptibility and dysregulated lipid metabolism are important susceptibilities to preeclampsia. In the study, we devote to investigate the associations of FOXO3 and TLR7 genetic polymorphisms with preeclampsia in a Chinese population.
Methods
This case-control study involved 335 Han Chinese pregnant women, including 177 pregnant women with preeclampsia and 158 healthy controls. The preeclampsia group was further sub-grouped into early-onset preeclampsia (EOPE, n=70）and late-onset preeclampsia (LOPE, n=107）. Three single nucleotide polymorphisms SNPs , including FOXO3 rs2232365, rs3761548, and TLR7 rs3853839 were genotyped by multiplex PCR for targeted next-generation sequencing. The χ2 test and multiple interaction effect analyses were performed to determine the association of three SNPs with serum lipid levels and thyroid function in women with preeclampsia.
Results
Our study showed that SNP rs2232365 C allele frequencies were significantly associated with the occurrence of LOPE (P=0.022, odds ratio = 1.72 (0.95 CI: 1.23-2.41)). The genotype (CC vs TT+CT) distribution of rs2232365 revealed a significant association with LOPE (P=0.004, odds ratio = 3.497 (0.95 CI: 1.498-8.164)). No significant difference was found in the genotype and allele frequencies of rs3761548 and rs3853839 between controls and cases (P>0.05). Moreover, the genotype CC of rs2232365 was significantly correlated with increased TG/HDL levels in the LOPE group (all p=0.014).
Conclusions
The polymorphisms of rs2232365 are associated with the risk of LOPE and may modulate TG/HDL levels in pregnant women with LOPE.
Figure 1
Figure 2