This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Linqing Zhao
Capital Institute of Pediatrics
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5622-1959
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background Human rhinovirus (HRV) infections were confirmed in severe acute lower respiratory infections (ALRI) . Methods To evaluate the diversity and circulation pattern of HRVs species, specimens were collected from pediatric patients with ALRI during Dec 2016 to Feb 2019 and screened by RVP FAST Assay. Specimens positive for HRVs/ Enteroviruses (EVs) were then tested for HRVs and EVs by PCR. Then the capsid protein gene of HRVs was amplified and sequenced for species identification, and clinical data of HRV infections were analyzed. The chi-square (χ 2 ) test and rank sum test was used for statistical analysis using SPSS Statistics 22.0 version. In order to test and evaluate the relationship between patients positive for different HRV species and the outcome of Intensive Care Unit (ICU) (ICU group) or Department of Respiratory admission (Respiratory group), a logistic model was constructed using multiple logistic regression analysis. Results Among 1835 specimens tested, 363 (19.8%) were positive for EVs/HRVs, and 314 (86.5%, 314/363) were positive for HRVs, including 177 (56.4%, 177/314) HRV A, 29 (9.2%, 29/314) HRV B, and 108 (34.4%, 108/314) HRV C. Using HRV C as the control species, HRV A was a dangerous factor for severe clinical outcome (OR=1.983, 95% CI=1.091-3.605) ( p =0.025). Patients positive for HRVs from ICU group were significantly younger than those from Respiratory group (0.39 years: 1.80 years, p =0.000) and age was confirmed as a protective factor for severe ALRI. In August and September, HRV A and B are the dominant species, then HRV C in October and December. Among 96 types confirmed in the study, the predominant types usually showed in no consecutive years. Conclusions HRVs were important viral pathogens for ALRI in children. HRV A and C are more popular. Compared to HRV C, HRV A were associated more powerful with severe ALRI. The risk of severe ALRI by HRVs infections is decreased with the increasing of age.
Keywords
Pediatric patients, acute lower respiratory infection, human rhinovirus, species, diversity
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 01 Nov, 2019
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Infectious Diseases
Learn more about our company.
This is a preprint. It has not completed peer review.
Research article
Linqing Zhao
Capital Institute of Pediatrics
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5622-1959
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 01 Nov, 2019
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Infectious Diseases
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background Human rhinovirus (HRV) infections were confirmed in severe acute lower respiratory infections (ALRI) . Methods To evaluate the diversity and circulation pattern of HRVs species, specimens were collected from pediatric patients with ALRI during Dec 2016 to Feb 2019 and screened by RVP FAST Assay. Specimens positive for HRVs/ Enteroviruses (EVs) were then tested for HRVs and EVs by PCR. Then the capsid protein gene of HRVs was amplified and sequenced for species identification, and clinical data of HRV infections were analyzed. The chi-square (χ 2 ) test and rank sum test was used for statistical analysis using SPSS Statistics 22.0 version. In order to test and evaluate the relationship between patients positive for different HRV species and the outcome of Intensive Care Unit (ICU) (ICU group) or Department of Respiratory admission (Respiratory group), a logistic model was constructed using multiple logistic regression analysis. Results Among 1835 specimens tested, 363 (19.8%) were positive for EVs/HRVs, and 314 (86.5%, 314/363) were positive for HRVs, including 177 (56.4%, 177/314) HRV A, 29 (9.2%, 29/314) HRV B, and 108 (34.4%, 108/314) HRV C. Using HRV C as the control species, HRV A was a dangerous factor for severe clinical outcome (OR=1.983, 95% CI=1.091-3.605) ( p =0.025). Patients positive for HRVs from ICU group were significantly younger than those from Respiratory group (0.39 years: 1.80 years, p =0.000) and age was confirmed as a protective factor for severe ALRI. In August and September, HRV A and B are the dominant species, then HRV C in October and December. Among 96 types confirmed in the study, the predominant types usually showed in no consecutive years. Conclusions HRVs were important viral pathogens for ALRI in children. HRV A and C are more popular. Compared to HRV C, HRV A were associated more powerful with severe ALRI. The risk of severe ALRI by HRVs infections is decreased with the increasing of age.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Loading...