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Research article
Single-cell Profiling Reveal Types of Interstitial Cells in Human and Rat Bladder
Longkun Li
Army Medical University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Backgrounds:
The mechanism underlying bladder urination pathophysiologies has not been understood yet because the types of interstitial cells present in the bladder are still obscure.
Results:
Here, we classified the cell types in the bladder without bias using 10x genomics single-cell RNA-seq and identified 35,510 and 19,946 cells from human and rat bladder tissues, respectively. In addition to the major cell clusters including urothelial cells, smooth muscle cells, endothelial cells, neurons, and immune cells, three types of interstitial cells were identified in this study. Fibroblasts and myofibroblasts were shown to occupy large proportions of interstitial cells, located mainly between the epithelial strata and muscle strata. A new type of interstitial cell, Mki67+, which was found both in human and rat bladder tissues, may participate in bladder disease development.
Conclusions:
Our investigation thus lays the ground work for identifying the cell types in bladder tissues and provides potential clues to understand abnormal bladder functions.
Keywords
Single-cell RNA-seq, Bladder, Interstitial cells
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This is a preprint. It has not completed peer review.
Research article
Single-cell Profiling Reveal Types of Interstitial Cells in Human and Rat Bladder
Longkun Li
Army Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 22 Sep, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Backgrounds:
The mechanism underlying bladder urination pathophysiologies has not been understood yet because the types of interstitial cells present in the bladder are still obscure.
Results:
Here, we classified the cell types in the bladder without bias using 10x genomics single-cell RNA-seq and identified 35,510 and 19,946 cells from human and rat bladder tissues, respectively. In addition to the major cell clusters including urothelial cells, smooth muscle cells, endothelial cells, neurons, and immune cells, three types of interstitial cells were identified in this study. Fibroblasts and myofibroblasts were shown to occupy large proportions of interstitial cells, located mainly between the epithelial strata and muscle strata. A new type of interstitial cell, Mki67+, which was found both in human and rat bladder tissues, may participate in bladder disease development.
Conclusions:
Our investigation thus lays the ground work for identifying the cell types in bladder tissues and provides potential clues to understand abnormal bladder functions.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8