This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research article
manling wang
Key Laboratory of Agroecology in Subtropical Region, Institute of Sbutropical Agriculture, Chinese Academy of Science
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2136-1900
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Grain yield is a polygenic trait influenced by environmental and genetic interactions at all growth stages of the cereal plant. However, the molecular mechanisms responsible for coordinating the trade-off or cross-talk between these traits remain elusive.
Results
We characterized the hitherto unknown function of four STRESS_tolerance and GRAIN_LENGTH (OsSGL) Poaceae ortholog genes, all encoding DUF1645 domain-containing proteins, in simultaneous regulation of grain length, grain weight, and drought stress-tolerance in rice. In normal growth conditions, the four ortholog genes were mainly expressed in the developing roots and panicles of the corresponding species. Over-expressing or heterologous high-level expressing Poaceae OsSGL ortholog genes conferred remarkably increased grain length, weight, and seed setting percentage, as well as significantly improved drought-stress tolerance in transgenic rice. Microscopical analysis also showed that the transgene expression promoted cell division and development. RNA-seq and qRT-PCR analyses revealed 73.8% (18,711) overlapped DEGs in all transgenic plants. Moreover, GO and KEGG analyses of different comparisons revealed that the key DEGs participating in drought stress-response belonged to hormone (especially auxin and cytokinin) pathways, and signaling processes were apparently affected in the young panicles.
Conclusion
Together, these results suggest the four OsSGL orthologs perform a conserved function in regulating stress-tolerance and cell growth by acting via a hormone biosynthesis and signaling pathway. It may also induce a strategy for tailor-made crop yield improvement.
Keywords
graminaceous cereal crop, OsSGL, DUF1645, drought resistance, grain length, grain weight
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 24 Nov, 2020
No community comments so far
Editorial decision: Withdrawn by author
On 08 Jul, 2021
Editor assigned
On 10 Nov, 2020
Submission checks complete
On 10 Nov, 2020
Editor invited
On 10 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
manling wang
Key Laboratory of Agroecology in Subtropical Region, Institute of Sbutropical Agriculture, Chinese Academy of Science
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2136-1900
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 24 Nov, 2020
No community comments so far
Editorial decision: Withdrawn by author
On 08 Jul, 2021
Editor assigned
On 10 Nov, 2020
Submission checks complete
On 10 Nov, 2020
Editor invited
On 10 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Grain yield is a polygenic trait influenced by environmental and genetic interactions at all growth stages of the cereal plant. However, the molecular mechanisms responsible for coordinating the trade-off or cross-talk between these traits remain elusive.
Results
We characterized the hitherto unknown function of four STRESS_tolerance and GRAIN_LENGTH (OsSGL) Poaceae ortholog genes, all encoding DUF1645 domain-containing proteins, in simultaneous regulation of grain length, grain weight, and drought stress-tolerance in rice. In normal growth conditions, the four ortholog genes were mainly expressed in the developing roots and panicles of the corresponding species. Over-expressing or heterologous high-level expressing Poaceae OsSGL ortholog genes conferred remarkably increased grain length, weight, and seed setting percentage, as well as significantly improved drought-stress tolerance in transgenic rice. Microscopical analysis also showed that the transgene expression promoted cell division and development. RNA-seq and qRT-PCR analyses revealed 73.8% (18,711) overlapped DEGs in all transgenic plants. Moreover, GO and KEGG analyses of different comparisons revealed that the key DEGs participating in drought stress-response belonged to hormone (especially auxin and cytokinin) pathways, and signaling processes were apparently affected in the young panicles.
Conclusion
Together, these results suggest the four OsSGL orthologs perform a conserved function in regulating stress-tolerance and cell growth by acting via a hormone biosynthesis and signaling pathway. It may also induce a strategy for tailor-made crop yield improvement.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
Loading...