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Article
Programming complex regulation mechanisms through simple molecular assembly
Alexis Vallee-Belisle
University of Montreal
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5009-7715
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This work is licensed under a CC BY 4.0 License. Read Full License
What are the advantages and disadvantages of building nanosystems using one or multiple components? More than 55% of all proteins found in living organisms are multimeric and likely exploit molecular assembly to create new functional entities. However, the specific contribution of molecular assembly to the creation of novel functions remains relatively unexplored at the thermodynamic, kinetic and molecular levels. Here, we use theory and a simple experimental model to determine the design rules for engineering efficient self-assembled, self-regulated nanosystems. Using these rules, we have rationally designed and implemented various regulation mechanisms (e.g., cooperative and anticooperative assembly, self-inhibition, molecular timer) into two model trimeric nanosystems including a complex artificial catalyst. These simple strategies based on molecular assembly have been extensively exploited by natural biosystems and are expected to play a crucial role in the development of future self-regulated nanotechnologies.
Keywords
complex regulation mechanisms, simple molecular assembly, nanosystems, nanotechnologies
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This is a preprint. It has not completed peer review.
Article
Programming complex regulation mechanisms through simple molecular assembly
Alexis Vallee-Belisle
University of Montreal
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5009-7715
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 17 Sep, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
What are the advantages and disadvantages of building nanosystems using one or multiple components? More than 55% of all proteins found in living organisms are multimeric and likely exploit molecular assembly to create new functional entities. However, the specific contribution of molecular assembly to the creation of novel functions remains relatively unexplored at the thermodynamic, kinetic and molecular levels. Here, we use theory and a simple experimental model to determine the design rules for engineering efficient self-assembled, self-regulated nanosystems. Using these rules, we have rationally designed and implemented various regulation mechanisms (e.g., cooperative and anticooperative assembly, self-inhibition, molecular timer) into two model trimeric nanosystems including a complex artificial catalyst. These simple strategies based on molecular assembly have been extensively exploited by natural biosystems and are expected to play a crucial role in the development of future self-regulated nanotechnologies.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the manuscript can be downloaded and accessed as a PDF.